| Project/Area Number |
23791911
|
|---|
| Research Category |
Grant-in-Aid for Young Scientists (B)
|
| Allocation Type | Multi-year Fund |
|---|
| Research Field |
Otorhinolaryngology
|
|---|
| Research Institution | Kagoshima University |
|---|
Principal Investigator |
|
|---|
| Project Period (FY) |
2011 – 2012
|
| Project Status |
Completed (Fiscal Year 2012)
|
| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2011: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
|
|---|
| Keywords | 粘膜ワクチン / Th1/Th2バランス / Phosphprylcholine / アレルギー / Phosphorylcholine / dendritic cells / Ag-presenting cells / ovalbumin / bovine serum albumin / NALT / PAFR / NTHi / PC(Phosphorylcholine) / OVA(Ovalbmin) / IL-4 / IFN-γ / Th1/Th2 |
|---|
| Research Abstract |
Background: Phosphorylcholine (PC), which is a structural component of wide variety of bacteria. However immunomodulatory properties of PC in the upper respiratory tract have not yet been characterized. Objective: We sought to elucidate the effects of PC on the regulation of allergic response in the upper respiratory tract. Methods: Allergic rhinitis model established by ovalbumin (OVA) sensitization was pretreated by intranasal or peritoneal administrationof PC-bovine serum albumin (PC-BSA). Allergic responses were compared with non-pretreated mice. IL-12p40 production by DCs stimulated with PC was measured to evaluate the effect of PC on the TH2 responses mediated by DCs. Results: PC-pretreated mice showed mild allergic nasal symptoms compared to the mice without PC-pretreatment. The levels of total IgE and OVA-specific IgE were significantly lower inPC-pretreated mice. The IL-4 production by splenic CD4+T cells was down-regulated by PC-pretreatment. Further, PC induced IL-12p40 production by DCs. Conclusion: These findings indicate that PC inhibits the allergic response in the upper respiratory tract mediated by the induction of IL-12p40 production by DCs. Thus, PC might be useful as an immunotherapeutic agent for allergic diseases.
|
