Studies on biological defense mechanism via the pattern recognition receptor for viral infection in upper airway epithelium.

• Project/Area Number: 23791916
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Otorhinolaryngology
• Research Institution: Sapporo Medical University
• Principal Investigator: OHUKUNI Tsuyoshi 札幌医科大学, 医学部, 助教 (40464490)
• Research Collaborator: HIMI Tetsuo 札幌医科大学, 医学部, 教授 (90181114)
• Project Period (FY): 2011 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2011: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
• Keywords: 鼻粘膜 / ウイルス / 受容体 / 上気道 / 生体防御 / 上皮 / 自然免疫 / タイト結合 / RSウイルス

Research Abstract

The airway epithelium is the first line of biological defense during respiratory viral infection. In the present study, we made analysis of innate immune system against early phase viral infection in experiments on human nasal epithelial cells (HNECs) infected with RS virus. In our experiments, these results indicated that 1) RIG-I may play the major role in the recognition of RS virus, 2) RS virus infection induces the secretion of type III interferon, 3) RS virus infection changes the localization of tight junction proteins, claudin4 and occludin.

Research Products

• [Journal Article] Regulation of tight junctions in upper airway epithelium (2013)
  • Author(s): Kojima T, Takano K, Himi T(全19名1, 3, 18番)
  • Journal Title: Biomed Res Int Volume: 2013 Pages: 947072-947072
  • DOI: 10.1155/2013/947072
  • Peer Reviewed
• [Journal Article] Hop water extract inhibits double-stranded RNA-induced thymic stromal lymphopoietin release from nasal epithelial cells (2012)
  • Author(s): Fuchimoto J, Kojima T, Kobayasi N, Ohkuni T, Ogasawara N, Masaki T, Obata K, Nomura K, Kondoh A, Shigyo T, Himi T, Sawada N.
  • Journal Title: Am J Rhinol Allergy Volume: 26(6) Pages: 433-438
  • Peer Reviewed
• [Journal Article] Altered expression of claudin-1, claudin-7, and tricellulin regardless of human papilloma virus infection in human tonsillar squamous cell carcinoma. (2011)
  • Author(s): Kondoh A, Takano K, Kojima T, Ohkuni T, Kamekura R, Ogasawara N, Go M, Sawada N, Himi T.
  • Journal Title: Acta Otolaryngol Volume: 131(8) Pages: 861-868
  • Peer Reviewed
• [Journal Article] Poly (I;C) reduces expression of JAM-A and induces secretion of IL-8 and TNF α via distinct NF-kB pathways in human nasal epithelial cells (2011)
  • Author(s): Ohkuni T, Kojima T, Ogasawara N, Masaki T, Fuchimoto J, Kamekura R, Koizumi J, Ichimiya S, Murata M, Tanaka S, Himi T, Sawada N
  • Journal Title: Toxicol Appl Pharmacol Volume: 250(1) Pages: 29-38
  • Peer Reviewed
• [Journal Article] Mucosal immune barrier and antigen -presenting system in human nasal epithelial cells (2011)
  • Author(s): Himi T, Takano K, Ogasawara N, Go M, Kurose M, Koizumi J, Kamekura R, Kondoh A, Ohkuni T, Masaki T, Kojima T, Sawada N, Tsutsumi H.
  • Journal Title: Adv Otorhinolaryngol. Volume: 72 Pages: 28-30
  • Peer Reviewed
• [Journal Article] A nuclear factor-kB signaling pathway via protein kinase C delta regulates replication of respiratory syncytial virus in polarized normal human nasal epithelial cells. (2011)
  • Author(s): Masaki T, Kojima T, Okabayashi T, Ogasawara N, Ohkuni T, Obata K, Takasawa A, Murata M, Tanaka S, Hirakawa S, Fuchimoto J, Ninomiya T, Fujii N, Tsutsumi H, Himi T, Sawada N
  • Journal Title: Mol Biol Cell Volume: 22(13) Pages: 2144-2156
  • Peer Reviewed
• [Journal Article] Epithelial barrier and antigen uptake in lymphoepithelium of human adenoids (2011)
  • Author(s): Ogasawara N, Kojima T, Go M, Takano K, Kamekura R, Ohkuni T, Koizumi J, Masaki T, Fuchimoto J, Obata K, Kurose M, Shintani T, Sawada N, Himi T.
  • Journal Title: Acta Otolaryngol Volume: 131(2) Pages: 116-123
  • Peer Reviewed
• [Presentation] 上気道粘膜上皮におけるウイルス関連受容体を介した生体防御機構の検討 (2012)
  • Author(s): 小幡和史,小島隆,正木智之,平川賢史,野村一顕,高澤啓,渕本潤,大國毅,横田伸一,澤田典均,氷見徹夫
  • Place of Presentation: 下関市
• [Presentation] Poly IC Stimulation Reduces Expression of JAM-A and Induces Proinflammatory Cytokines Secretion via Distinct NF-kB Pathways in Human Nasal Epithelial Cells. (2011)
  • Author(s): Ohkuni T, Himi T, Sawada N, Kojima T, Kamekura R, Ogasawara N, Masaki T.
  • Organizer: XIV IRS, XXX ISIAN 2011
  • Place of Presentation: 東京都
• [Presentation] A NF-kappa B Signaling Pathway via PKC-delta Regulates Replication of Respiratory Syncytial Virus in Polarized Normal Human Nasal Epithelial Cells (2011)
  • Author(s): Masaki T, Kojima T, Ogasawara N, Ohkuni T, Obata K, Fuchimoto J, Sawada N, Himi T.
  • Organizer: XIV IRS, XXX ISIAN 2011
  • Place of Presentation: 東京都
• [Presentation] Poly IC stimulation reduces expression of JAM-A and induces proinflammatory cytokines secretion via distinct NF-kB pathways in human nasal epithelial cells.
  • Author(s): 大國 毅
  • Organizer: ISIAN 2011
  • Place of Presentation: 東京