Development of a Hybrid Three-Dimensional Culture Method in Regenerative Medicine

Research Project

Project/Area Number	23791917
Research Category	Grant-in-Aid for Young Scientists (B)
Allocation Type	Multi-year Fund
Research Field	Otorhinolaryngology
Research Institution	Fukushima Medical University
Principal Investigator	SUZUKI TERUHISA 福島県立医科大学, 医学部, 研究員 (80508812)
Co-Investigator(Renkei-kenkyūsha)	SUZUKI Masahiro 福島県立医科大学, 医学部, 助手 (90513268)
Project Period (FY)	2011 – 2013
Project Status	Completed (Fiscal Year 2013)
Budget Amount *help	¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000) Fiscal Year 2013: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000) Fiscal Year 2012: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000) Fiscal Year 2011: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Keywords	移植 / 再生医療 / 移植・再生医療
Research Abstract	Adipose Derived Stem Cells (ASC) and fibroblasts each promote the regeneration of the tracheal epithelial layer. In this study, we created a 3D Architecture of cell layered arrangement (3D-A) in order to simultaneously make use of the strong points of ASC and fibroblasts. Upon grafting of 3D-A into the tracheal injury model of a rat, we were able to confirm the regeneration of a functional tracheal epithelial layer to near its normal morphology in a shorter time than with grafting of ASC or fibroblasts alone. The development of this hybrid 3D culture method seems to be capable of contributing greatly to trachea regenerative medicine.