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Observation of limbal stem cell niche using in vivo confocal microscope

Research Project

Project/Area Number 23791962
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Ophthalmology
Research InstitutionOsaka University

Principal Investigator

OIE Yoshinori  大阪大学, 医学(系)研究科(研究院), 助教 (20599881)

Project Period (FY) 2011 – 2013
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2013: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2012: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2011: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Keywords眼発生・再生医学 / 角膜上皮幹細胞 / 再生医学 / 幹細胞 / 移植・再生医療
Research Abstract

Longest diameter and dimension of limbal basal cells were evaluated using in vivo confocal miscroscope (Heidelberg Retina Tomograph 2 Rostock Corneal Module) for patients with Harada disease. Sixteen eyes of nine patients were included. Average age was 51, 13 eyes with men, 3 eyes with women. Longest diameter and dimension of limbal basal cells in patients with Harada disease were 16.1um and 135um2, respectively. It was suggested that limbal basal cells in Harada disease were larger than in normal eyes because of destruction of melanocyte in limbal niche.

Report

(4 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Research-status Report
  • 2011 Research-status Report
  • Research Products

    (41 results)

All 2014 2013 2012 2011

All Journal Article (7 results) (of which Peer Reviewed: 2 results) Presentation (26 results) (of which Invited: 2 results) Book (6 results) Patent(Industrial Property Rights) (2 results)

  • [Journal Article] Rigid gas permeable contact lens-assisted cataract surgery in patients with severe keratoconus2014

    • Author(s)
      Oie Y, Kamei M, Matsumura N, Fujimoto H, Soma T, Koh S, Tsujikawa M, Maeda Nishida K
    • Journal Title

      J Cataract Refract Surg

      Volume: 40 Pages: 345-8

    • Related Report
      2013 Final Research Report
  • [Journal Article] Development of a cell sheet transportation technique for regenerative medicine2014

    • Author(s)
      Oie Y, Nozaki T, Takayanagi H, Hara S, Hayashi R, Takeda S, Mori K, Moriya N, Soma T, Tsujikawa M, Saito K, Nishida K
    • Journal Title

      Tissue Eng Part C Methods

      Volume: 20 Pages: 373-82

    • Related Report
      2013 Final Research Report
  • [Journal Article] ヒト臨床試験のための培養口腔粘膜上皮細胞シートの輸送技術開発2014

    • Author(s)
      大家義則
    • Journal Title

      再生医療

      Volume: 13 Pages: 58-63

    • Related Report
      2013 Final Research Report
  • [Journal Article] Rigid gas-permeable contact lens-assisted cataract surgery in patients with severe keratoconus.2014

    • Author(s)
      Oie Y, Kamei M, Matsumura N, Fujimoto H, Soma T, Koh S, Tsujikawa M, Maeda N, Nishida K.
    • Journal Title

      J Cataract Refract Surg.

      Volume: 40 Pages: 345-8

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Regenerative medicine for the cornea2013

    • Author(s)
      Oie Y, Nishida K
    • Journal Title

      Biomed Res Int

      Volume: 2013 Pages: 428247-428247

    • Related Report
      2013 Final Research Report
  • [Journal Article] Effect of instillation of eyedrops for dry eye on optical quality2013

    • Author(s)
      Koh S, Maeda N, Ikeda C, Takai Y, Fujimoto H, Oie Y, Soma T, Tsujikawa M, Nishida K
    • Journal Title

      Invest Ophthalmol Vis Sci

      Volume: 54 Pages: 4927-33

    • Related Report
      2013 Annual Research Report 2013 Final Research Report
  • [Journal Article] Regenerative medicine for the cornea2013

    • Author(s)
      Oie Y, Nishida K.
    • Journal Title

      Biomed Res Int.

      Volume: 未定 Pages: 1-8

    • DOI

      10.1155/2013/428247

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Presentation] タイ王国における培養口腔粘膜上皮細胞シートを用いた角膜上皮再生治療および遠隔診療の実施2014

    • Author(s)
      大家義則、小林由紀、本田愛、藤本久貴、中尾武史、橋田徳康、林竜平、相馬剛至、辻川元一、西田幸二
    • Organizer
      第13回再生医療学会総会
    • Place of Presentation
      京都府(口演)
    • Year and Date
      2014-03-05
    • Related Report
      2013 Final Research Report
  • [Presentation] タイ王国での角膜上皮再生医療と遠隔診療の実施2014

    • Author(s)
      大家義則、小林由紀、本田愛、藤本久貴、中尾武史、橋田徳康、林竜平、相馬剛至、辻川元一、西田幸二
    • Organizer
      角膜カンファランス2014
    • Place of Presentation
      沖縄(ポスター)
    • Year and Date
      2014-01-30
    • Related Report
      2013 Final Research Report
  • [Presentation] Ocular surface reconstruction using oral mucosal epithelial cell sheet2014

    • Author(s)
      Yoshinori Oie
    • Organizer
      Qatar-Japan Symposium on Tissue Regenerative therapy
    • Place of Presentation
      Doha, カタール
    • Year and Date
      2014-01-27
    • Related Report
      2013 Final Research Report
  • [Presentation] Development of tissue and cell sheet transportation technique for regenerative medicine.2014

    • Author(s)
      Yoshinori Oie, Satoru Andojo, Hiroshi Takayanagi, Susumu Hara, Ryuhei Hayashi, Takayuki Nozaki, Shizu Takeda, Takeshi Soma, Motokazu Tsujikawa, Kohji Nishida.
    • Organizer
      Gordon Research Conference Cornea.
    • Place of Presentation
      Ventura(アメリカ合衆国)
    • Related Report
      2013 Annual Research Report
  • [Presentation] Ocular surface reconstruction using oral mucosal epithelial cell sheet.2014

    • Author(s)
      Yoshinori Oie.
    • Organizer
      Qatar-Japan Symposium on Tissue Regenerative therapy
    • Place of Presentation
      ドーハ(カタール)
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] タイ王国での角膜上皮再生医療と遠隔診療の実施2014

    • Author(s)
      大家義則、小林由紀、本田愛、藤本久貴、中尾武史、橋田徳康、林竜平、相馬剛至、辻川元一、西田幸二
    • Organizer
      角膜カンファランス2014
    • Place of Presentation
      沖縄
    • Related Report
      2013 Annual Research Report
  • [Presentation] タイ王国における培養口腔粘膜上皮細胞シートを用いた角膜上皮再生治療および遠隔診療の実施2014

    • Author(s)
      大家義則、小林由紀、本田愛、藤本久貴、中尾武史、橋田徳康、林竜平、相馬剛至、辻川元一、西田幸二
    • Organizer
      第13回再生医療学会総会
    • Place of Presentation
      京都
    • Related Report
      2013 Annual Research Report
  • [Presentation] Ocular surface reconstruction using human oral mucosal epithelial cell sheets2013

    • Author(s)
      Yoshinori Oie
    • Organizer
      The 19^<th> annual meeting of Kyoto Cornea Club 2013
    • Place of Presentation
      京都府(口演)
    • Year and Date
      2013-11-30
    • Related Report
      2013 Final Research Report
  • [Presentation]2013

    • Author(s)
      Yoshinori Oie, Takeshi Soma, Ryuhei Hayashi, Motokazu Tsujikawa, Kohji Nishida
    • Organizer
      Termis-AP Meeting 2013
    • Place of Presentation
      Wuzhen, 中国(口演)
    • Year and Date
      2013-10-25
    • Related Report
      2013 Final Research Report
  • [Presentation] 自家培養口腔粘膜上皮細胞シート移植の多施設共同臨床試験2013

    • Author(s)
      大家義則
    • Organizer
      第117回日本眼科学会
    • Place of Presentation
      東京都(モーニングセミナー)
    • Year and Date
      2013-04-06
    • Related Report
      2013 Final Research Report
  • [Presentation] 臨床試験のための口腔粘膜組織および培養口腔粘膜上皮細胞シートの空輸技術開発2013

    • Author(s)
      大家義則、安堵城悟、金野なつみ、高柳泰、野崎貴之、武田志津、林竜平、相馬剛至、辻川元一、西田幸二
    • Organizer
      第117回日本眼科学会
    • Place of Presentation
      東京都(ポスター)
    • Year and Date
      2013-04-05
    • Related Report
      2013 Final Research Report
  • [Presentation] 臨床試験を目指した口腔粘膜組織および培養口腔粘膜上皮細胞シートの空輸技術開発2013

    • Author(s)
      大家義則、安堵城悟、金野なつみ、高柳泰、野崎貴之、武田志津、林竜平、相馬剛至、辻川元一、西田幸二
    • Organizer
      第12回再生医療学会総会
    • Place of Presentation
      神奈川県(口演)
    • Year and Date
      2013-03-22
    • Related Report
      2013 Final Research Report
  • [Presentation] ヒト臨床試験のための培養口腔粘膜上皮細胞シートの輸送技術開発2013

    • Author(s)
      大家義則、野崎貴之、金野なつみ、高柳泰、原進、武田志津、林竜平、相馬剛至、辻川元一、西田幸二
    • Organizer
      第12回日本再生医療学会総会
    • Place of Presentation
      神奈川県(YIA受賞者講演)
    • Year and Date
      2013-03-21
    • Related Report
      2013 Final Research Report
  • [Presentation] Regenerative medicine for cornea2013

    • Author(s)
      Yoshinori Oie
    • Organizer
      APAO2013
    • Place of Presentation
      Hyderabad, インド(口演)
    • Year and Date
      2013-01-18
    • Related Report
      2013 Final Research Report
  • [Presentation] Development of tissue and cell sheet transportation technique for regenerative medicine. Air transport system of oral mucosal tissue and cell sheets for clinical trial2013

    • Author(s)
      Yoshinori Oie, Satoru Andojo, Natsumi Konno, Hiroshi Takayanagi, Takayuki Nozaki, Shizu Takeda, Takeshi Soma, Motokazu Tsujikawa, Kohji Nishida
    • Organizer
      ARVO (the association for research in vision and ophthalmology) annual meeting
    • Place of Presentation
      Seattle, アメリカ合衆国(口演)
    • Related Report
      2013 Final Research Report
  • [Presentation] Observation of meibomian gland in patients with limbal stem-cell deficiency using non-contact meibography2013

    • Author(s)
      Yoshinori Oie, Shizuka Koh, Hisataka Fujimoto, Takeshi Soma, Motokazu Tsujikawa, Naoyuki Maeda, Kohji Nishida
    • Organizer
      American Academy of Ophthalmology
    • Place of Presentation
      New Orleans(アメリカ合衆国)
    • Related Report
      2013 Annual Research Report
  • [Presentation] 臨床試験を目指した口腔粘膜組織および培養上皮細胞シートの空輸技術開発2013

    • Author(s)
      大家義則、安堵城悟、金野なつみ、高柳泰、野崎貴之、武田志津、林竜平、相馬剛至、辻川元一、西田幸二
    • Organizer
      第12回日本再生医療学会総会
    • Place of Presentation
      パシフィコ横浜
    • Related Report
      2012 Research-status Report
  • [Presentation] Cell transport system for clinical trial using cultured human oral mucosal epithelial cell sheets2012

    • Author(s)
      Yoshinori Oie、Ryuhei Hayashi、Hiroshi Takayanagi、Susumu Hara、Takayuki Nozaki、Shizu Takeda、Takeshi Soma、Motokazu Tsujikawa、Kohji Nishida
    • Organizer
      ISSCR annual meeting
    • Place of Presentation
      神奈川県(ポスター)
    • Year and Date
      2012-06-15
    • Related Report
      2013 Final Research Report
  • [Presentation] ヒト臨床試験のための培養口腔粘膜上皮細胞シートの輸送技術開発2012

    • Author(s)
      大家義則、林竜平、高柳泰、原進、野崎貴之、武田志津、相馬剛至、辻川元一、西田幸二
    • Organizer
      第11回日本再生医療学会総会
    • Place of Presentation
      神奈川県(一般口演)
    • Year and Date
      2012-06-12
    • Related Report
      2013 Final Research Report
  • [Presentation] 培養口腔粘膜上皮細胞シートの輸送システム開発2012

    • Author(s)
      大家義則、林竜平、高柳泰、原進、野崎貴之、武田志津、相馬剛至、辻川元一、西田幸二
    • Organizer
      角膜カンファランス2012
    • Place of Presentation
      東京都(一般)
    • Year and Date
      2012-02-23
    • Related Report
      2013 Final Research Report
  • [Presentation] ヒト臨床試験のための培養口腔粘膜上皮細胞シートの輸送技術開発2012

    • Author(s)
      大家義則、林竜平、高柳泰、原進、野崎貴之、武田志津、相馬剛至、辻川元一、西田幸二
    • Organizer
      第11回日本再生医療学会総会
    • Place of Presentation
      パシフィコ横浜
    • Related Report
      2012 Research-status Report
  • [Presentation] Cell transport system for clinical trial using cultured human oral mucosal epithelial cell sheets2012

    • Author(s)
      Yoshinori Oie、Ryuhei Hayashi、Hiroshi Takayanagi、Susumu Hara、Takayuki Nozaki、Shizu Takeda、Takeshi Soma、Motokazu Tsujikawa、Kohji Nishida
    • Organizer
      ISSCR annual meeting
    • Place of Presentation
      パシフィコ横浜
    • Related Report
      2012 Research-status Report
  • [Presentation] Cell transport technique by airplane for clinical trial using tissue-engineered human oral mucosal epithelial cell sheets2012

    • Author(s)
      Yoshinori Oie
    • Organizer
      The 1st Forum of Harvard-Osaka-Xiamen universities
    • Place of Presentation
      Xiamen University
    • Related Report
      2012 Research-status Report
    • Invited
  • [Presentation] 培養口腔粘膜上皮細胞シートの輸送システム開発2012

    • Author(s)
      大家義則、林竜平、高柳泰、原進、野崎貴之、武田志津、相馬剛至、辻川元一、西田幸二
    • Organizer
      角膜カンファランス2012
    • Place of Presentation
      東京都
    • Related Report
      2011 Research-status Report
  • [Presentation] Stem cells and regenerative medicine for cornea2011

    • Author(s)
      Yoshinori Oie
    • Organizer
      The Cornea and Tissue Engineering A JSPS-sponsored research symposium at Cardiff University(招待講演)
    • Place of Presentation
      英国カーディフ市
    • Related Report
      2011 Research-status Report
  • [Presentation] Ocular surface reconstruction with tissue-engineered cell sheets using oral mucosal epithelium2011

    • Author(s)
      Yoshinori Oie
    • Organizer
      APACRS(招待講演)
    • Place of Presentation
      韓国ソウル市
    • Related Report
      2011 Research-status Report
  • [Book] "角結膜瘢痕の鑑別", オキュラーサーフェス疾患(眼科臨床エキスパート)2013

    • Author(s)
      大家義則
    • Publisher
      医学書院
    • Related Report
      2013 Final Research Report
  • [Book] 角膜上皮の再生医療 眼科手術2013

    • Author(s)
      大家義則
    • Related Report
      2013 Final Research Report
  • [Book] "培養上皮細胞シート移植", 角膜・結膜・屈折矯正(眼手術学4)2013

    • Author(s)
      大家義則
    • Publisher
      文光堂
    • Related Report
      2013 Final Research Report
  • [Book] "角膜(総説)", 上皮・感覚器(再生医療叢書4)2013

    • Author(s)
      大家義則、西田幸二
    • Publisher
      朝倉書店
    • Related Report
      2013 Final Research Report
  • [Book] "角膜(角膜上皮の再生)", 上皮・感覚器(再生医療叢書4)2013

    • Author(s)
      大家義則、西田幸二
    • Publisher
      朝倉書店
    • Related Report
      2013 Final Research Report
  • [Book] 再生医療叢書(上皮・感覚器)2013

    • Author(s)
      大家義則、西田幸二
    • Total Pages
      250
    • Publisher
      朝倉書店
    • Related Report
      2012 Research-status Report
  • [Patent(Industrial Property Rights)] 包装容器およびそれを用いた試料観察方法2011

    • Inventor(s)
      野崎貴之、西田幸二、森圭祐、大家義則、林竜平
    • Industrial Property Rights Holder
      野崎貴之、西田幸二、森圭祐、大家義則、林竜平
    • Industrial Property Number
      2011-179492
    • Filing Date
      2011
    • Related Report
      2011 Research-status Report
  • [Patent(Industrial Property Rights)] 細胞輸送容器2011

    • Inventor(s)
      野崎貴之、西田幸二、森圭祐、大家義則、林竜平
    • Industrial Property Rights Holder
      野崎貴之、西田幸二、森圭祐、大家義則、林竜平
    • Industrial Property Number
      2011-179491
    • Filing Date
      2011
    • Related Report
      2011 Research-status Report

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Published: 2011-08-05   Modified: 2019-07-29  

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