The role of mu-crystallin in laser-induced choroidal neovascularization

• Project/Area Number: 23791973
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Ophthalmology
• Research Institution: Shinshu University
• Principal Investigator: IMAI Hiroki 信州大学, 医学部, 助教 (50568409)
• Project Period (FY): 2011 – 2013
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2013: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2011: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
• Keywords: ミュークリスタリン / 脈絡膜新生血管 / 加齢黄斑変性 / レーザー誘発脈絡膜新生血管

Research Abstract

Choroidal neovascularization model was induced by laser photocoagulation(125 micrometer spot size, 130mW intensity, 50ms duration; 80nm; Oculight SLx; Iris Instruments, Mountain View, CA) performed on one eye of male C57BL6, mu-crystallin knockout, and wild-type mice (8 to 12 weeks of ages)choroid-sclera flatmount was stained by anti-isolectin antibody. The volume and area of neovascularization were measured by laser confocal microscopy (TCS SP2; Leica). The mean volume or area of choroidal neovascularization was not significantly different from mu-crystallin knockout mice and wild-type mice.