The neuroprotection for photoreceptor apoptosis via selective antagonist, BBG

• Project/Area Number: 23791991
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Ophthalmology
• Research Institution: 国立病院機構九州医療センター (2012); National Hospital Organization, Kyushu Medical Center (Clinical Institute) (2011)
• Principal Investigator: HISATOMI Toshio 国立病院機構九州医療センター, 眼科, 科長 (50404033)
• Project Period (FY): 2011 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2012: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2011: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
• Keywords: 眼細胞生物学

Research Abstract

The retinal cells developed apoptosis via P2X7 receptor dependent pathways. Cell death developed via rapid Ca++ intake into the cells and followed by apoptotic molecular signals such as membrane permeabilization, caspase activation, cytochrome c and AIF translocation. P2X7 receptor knockout mice showed decreased cell death without P2X7 receptor. The cell death and following molecular signal transduction were reversed by selective antagonist, BBG.

Research Products

• [Journal Article] Dynamic increase in extracellular ATP accelerates photoreceptor cell apoptosis via ligation of P2RX7 in subretinal hemorrhage. (2013)
  • Author(s): Notomi S,Hisatomi T.et al.
  • Journal Title: PLoS One Volume: 8(1)
  • Peer Reviewed
• [Journal Article] The regulatory roles of apoptosis-inducing factor in the formation and regression processes of ocular neovascularization. (2012)
  • Author(s): 久冨智朗
  • Journal Title: Am J Pathol. Volume: 181(1) Pages: 53-61
  • Peer Reviewed
• [Journal Article] MutT homolog-1 attenuates oxidative DNA damage and delays photoreceptor cell death in inherited retinal degeneration. (2012)
  • Author(s): Notomi S,Hisatomi T.et al.
  • Journal Title: Am J Pathol. Volume: 181(4) Pages: 1378-86
  • Peer Reviewed
• [Journal Article] The regulatory roles of apoptosis inducing factor in the formation and regresion processes of ocular neovascularization. (2012)
  • Author(s): Hisatomi T,Notomi S,et al.
  • Journal Title: Am J Pathol Volume: 180
  • Peer Reviewed
• [Journal Article] Critical Involvement of Extracellular ATP Acting on P2RX7 Purinergic Receptors in Photoreceptor Cell Death. (2011)
  • Author(s): Notomi S,Hisatomi T,et al.
  • Journal Title: Am J Pathol Volume: 179 Pages: 2798-2809
  • NAID: 120005133085
  • Peer Reviewed
• [Presentation] 眼病理から考える高度近視の病態 (2012)
  • Author(s): 久冨 智朗
  • Organizer: 第１１６回日本眼科学会総会
  • Place of Presentation: 東京
• [Presentation] マウス網膜下出血モデルにおけるブリリアントブルーＧの効果 (2012)
  • Author(s): 納富 昭司、久冨智朗
  • Organizer: 第１１６回日本眼科学会総会
  • Place of Presentation: 東京
• [Presentation] Ｐｈｏｔｏｒｅｃｅｐｔｏｒ ａｐｏｐｔｏｓｉｓ ｉｎ ｒｅｔｉｎａｌ ｄｉｓｅａｓｅｓ (2012)
  • Author(s): 久冨 智朗
  • Organizer: 第１１６回日本眼科学会総会 シンポジウム
  • Place of Presentation: 東京
• [Presentation] ＢＢＧと神経保護作用 (2012)
  • Author(s): 納富 昭司、久冨智朗
  • Organizer: 第１回 ＨＭＳ Ｊａｐａｎ
  • Place of Presentation: 東京
• [Presentation] Macular Holes-Update on Techniques and Tamponades (2012)
  • Author(s): 久冨 智朗
  • Organizer: Ｔｈｅ ２７th APAO Ｃｏｎｇｒｅｓｓ
  • Place of Presentation: 釜山
• [Presentation] High biocompatibility of BBG depends on low cellular permeabillity into the live cells. (2012)
  • Author(s): 久冨 智朗
  • Organizer: World Ophthalmology Confence 2012(招待講演)
  • Place of Presentation: アブダビ
• [Presentation] Brilliant Blue G の細胞染色性と細胞膜透過性の検討 (2011)
  • Author(s): 久冨 智朗
  • Organizer: 日本眼科学会(招待講演)
  • Place of Presentation: 東京
• [Presentation] High biocompatibility of BBG depends on low cellular permeabillity into the live cells. (2011)
  • Author(s): 久冨 智朗
  • Organizer: ＡＲＶＯ
  • Place of Presentation: フロリダ
• [Presentation] Neuroprotective Effect Of Brilliant Blue G As A Selective P2x7 Receptor Antagonist In Retina (2011)
  • Author(s): 納富 昭司
  • Organizer: ARVO
  • Place of Presentation: フロリダ
• [Presentation]
  • Organizer: 第116回日本眼科学会総会
• [Presentation]
  • Organizer: 27th APAO Congress