Neuro protective effect of siRNA at glaucoma model rats

• Project/Area Number: 23791992
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Ophthalmology
• Research Institution: Saga University
• Principal Investigator: ISHIKAWA Shinichiro 佐賀大学, 医学部, 助教 (00404129)
• Project Period (FY): 2011 – 2013
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000); Fiscal Year 2013: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2012: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2011: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
• Keywords: 緑内障 / 神経保護 / siRNA / 眼科 / 虚血再灌流 / アポトーシス

Research Abstract

SiRNA(small interfering RNA) is a small molecule double-strand RNA and suppress specific gene expression. In this study, we investigated the effect of intra-vitreous injection of caspase-3 siRNA or caspase-9 siRNA against for retinal ganglion cell damage by transient ischemic injury, one of the rat's glaucoma models. In caspase-3 or caspase-9 injected eyes, loss of retinal ganglion cell was statistically smaller than the control group. Therefor capase-3 siRNA and capase-9 siRNA are expected for novel anti-glaucoma siRNA drug, prevent from retinal ganglion cell loosing by glaucoma.

Research Products

• [Journal Article] Neuroprotective Effect of Small Interfering RNA Targeted to Caspase-3 on Rat Retinal Ganglion Cell Loss Induced by Ischemia and Reperfusion Injury (2012)
  • Author(s): Shinichiro Ishikawa, Akira Hirata, Jo Nakabayashi, Ryo Iwakiri, Satoshi Okinami
  • Journal Title: Current Eye Research Volume: 37 Issue: 10 Pages: 907-913
  • DOI: 10.3109/02713683.2012.688161
  • Peer Reviewed
• [Presentation] Neuroprotective effects of siRNA, targeted caspase3, on rat retinal damage induced by different time course of transient ischemic injury (2013)
  • Author(s): Ishikawa S, Hirata A, Nakabayashi J, Iwakiri R, Okinami S
  • Place of Presentation: シアトル米国
  • Year and Date: 2013-05-08
• [Presentation] caspase3に対するsiRNAの網膜視神経節細胞保護効果の虚血時間による検討 (2013)
  • Author(s): 石川慎一郎・平田憲・中林條・岩切亮・沖波聡
  • Organizer: 第117回日本眼科学会
  • Place of Presentation: 東京
  • Year and Date: 2013-04-04
• [Presentation] Neuroprotective Effects Of siRNA, Targeted Caspase 3 On Rat Retinal Damage Induced By Different time course of Transient Ischemic Injury. (2013)
  • Author(s): Shinichiro Ishikawa, Jo Nakabayashi, Akira Hirata, Ryo Iwakiri, Satoshi Okinami
  • Organizer: ARVO
  • Place of Presentation: 米国 シアトル
• [Presentation] Neuroprotective effects of siRNA, targeted caspase9, and atelocollagen complex on rat retinal damage induced by transient ischemic injury (2012)
  • Author(s): Ishikawa S, Hirata A, Nakabayashi J, Iwakiri R, Okinami S
  • Place of Presentation: フロリダ米国
  • Year and Date: 2012-05-10
• [Presentation] アテロコラーゲンとcaspase9に対するsiRNA複合体による神経保護効果 (2012)
  • Author(s): 石川慎一郎・平田憲・中林條・岩切亮・沖波聡
  • Organizer: 第116回日本眼科学会
  • Place of Presentation: 東京
  • Year and Date: 2012-04-06
• [Presentation] アテロコラーゲンとcaspase3 に対するsiRNA の混合物 による視神経保護効果の検討 (2011)
  • Author(s): 石川慎一郎、平田憲、中林條、岩切亮、沖波聡
  • Organizer: 日本眼科学会
  • Place of Presentation: 東京
• [Presentation] Neuroprotective Effects Of Sirna, Targeted Caspase9, And Atelocollagen Complex On Rat Retinal Damage Induced By Transient Ischemic Injury.
  • Author(s): Shinichiro Ishikawa, Akira Hirata, Ryo Iwakiri, Jo Nakabayashi, Satoshi Okinami
  • Organizer: ARVO
  • Place of Presentation: Florida, USA.