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Comprehensive investigation of tension-sensitive factors that related to changes in cytoskeleton and cell adhesion in trabecular meshwork cells

Research Project

Project/Area Number 23791994
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Ophthalmology
Research InstitutionKumamoto University

Principal Investigator

INOUE Toshihiro  熊本大学, 医学部附属病院, 講師 (00317025)

Project Period (FY) 2011 – 2013
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2013: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2011: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Keywords緑内障 / 線維柱帯 / 酸化ストレス / 圧感受性 / 細胞骨格 / 房水 / 線維柱体 / シュレム管内皮
Research Abstract

We made a device to add hydrostatic pressure on cultured cells, resulting in changes in cell morphology even in the control condition, and the data were not analyzable. After treatment of trabecular meshwork cells with Y-27632, 5 genes that were related to antioxidant functions were up-regulated. Of those, the up-regulation of catalase was confirmed by realtime RT-PCR. Pretreatment with Y-27632 increased the signal of WST-8 assay and decreased the signal of DCFDA test reagent under stimulation with hydrogen peroxide. Collectively, it was indicated that ROCK inhibitor induced intracellular expression of antioxidants in the trabecular meshwork cells, and thereby suppressed oxidative stress-induced cell death.

Report

(4 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Research-status Report
  • 2011 Research-status Report
  • Research Products

    (52 results)

All 2014 2013 2012 2011 Other

All Journal Article (27 results) (of which Peer Reviewed: 26 results) Presentation (23 results) Remarks (2 results)

  • [Journal Article] Epithelial mesenchymal transition-like phenomenon in trabecular meshwork cells2014

    • Author(s)
      Takahashi E, Inoue T, Fujimoto T, Kojima S, Tanihara H
    • Journal Title

      Experimental Eye Research

      Volume: 118 Pages: 72-79

    • DOI

      10.1016/j.exer.2013.11.014

    • Related Report
      2013 Annual Research Report 2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Monocyte chemotactic protein-1 level in the aqueous humour as a prognostic factor for the outcome of trabeculectomy2014

    • Author(s)
      Inoue T, Kawaji T, Tanihara H
    • Journal Title

      Clinical and Experimental Ophthalmology

      Volume: 印刷中

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Trabeculectomy for open-angle glaucoma in phakic eyes vs in pseudophakic eyes after phacoemulsification : a prospective clinical cohort study2014

    • Author(s)
      Takihara Y, Inatani M, Ogata-Iwao M, Kawai M, Inoue T, Iwao K, Tanihara H
    • Journal Title

      JAMA Ophthalmology

      Volume: 132 Issue: 1 Pages: 69-76

    • DOI

      10.1001/jamaophthalmol.2013.5605

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Monocyte chemotactic protein-1 level in the aqueous humor as a prognostic factor for the outcome of trabeculectomy2013

    • Author(s)
      Inoue T, Kawaji T, Tanihara H
    • Journal Title

      Clinical and Experimental Ophthalmology

      Volume: (in press) Issue: 4 Pages: 334-341

    • DOI

      10.1111/ceo.12204

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Oxidative stress response signaling pathways in trabecular meshwork cells and their effects on cell viability2013

    • Author(s)
      Awai-Kasaoka N, Inoue T, Kameda T, Fujimoto T, Inoue-Mochita M, Tanihara H
    • Journal Title

      Molecular Vision

      Volume: 19 Pages: 1332-1340

    • Related Report
      2013 Annual Research Report 2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Elevated levels of multiple biomarkers of Alzheimer's disease in the aqueous humor of eyes with open-angle glaucoma2013

    • Author(s)
      Inoue T, Kawaji T, Tanihara H
    • Journal Title

      Investigative Ophthalmology & Visual Science

      Volume: 54 Issue: 8 Pages: 5353-5358

    • DOI

      10.1167/iovs.13-12245

    • Related Report
      2013 Annual Research Report 2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] The effect of Rho-associated kinase inhibition on the ocular penetration of timolol maleate2013

    • Author(s)
      Arnold JJ, Hansen MS, Gorman GS, Inoue T, Rao V, Spellen S, Hunsinger RN, Chapleau CA, Pozzo-Miller L, Stamer WD, Challa P
    • Journal Title

      Investigative Ophthalmology and Visual Science

      Volume: 54 Issue: 2 Pages: 1118-1126

    • DOI

      10.1167/iovs.12-10583

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Rho-associated kinase inhibitors: a novel glaucoma therapy2013

    • Author(s)
      Inoue T, Tanihara H
    • Journal Title

      Progress in Retinal and Eye Research

      Volume: 37 Pages: 1-12

    • DOI

      10.1016/j.preteyeres.2013.05.002

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Phase 2 randomized clinical study of a Rho kinase inhibitor, K-115, in primary open-angle glaucoma and ocular hypertension2013

    • Author(s)
      Tanihara H, Inoue T, Yamamoto T, Kuwayama Y, Abe H, Araie M; K-115 Clinical Study Group
    • Journal Title

      American Journal of Ophthalmology

      Volume: 156 Issue: 4 Pages: 731-736

    • DOI

      10.1016/j.ajo.2013.05.016

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Risk factors for choroidal detachment after trabeculectomy with mitomycin C2013

    • Author(s)
      Haga A, Inatani M, Shobayashi K, Kojima S, Inoue T, Tanihara H
    • Journal Title

      Clinical Ophthalmology

      Volume: 7 Pages: 1417-1421

    • DOI

      10.2147/opth.s46375

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Study on prognostic factor in trabeculectomy with mitomycin C having history of previous glaucoma surgery2013

    • Author(s)
      Awai-Kasaoka N, Inoue T, Inatani M, Takihara Y, Ogata-Iwao M, Tanihara H
    • Journal Title

      Japanese Journal of Ophthalmology

      Volume: 57 Issue: 6 Pages: 514-519

    • DOI

      10.1007/s10384-013-0257-5

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] A growth-promoting signaling component cyclin D1 in neural stem cells has anti-astrogliogenic function to execute self-renewal.2013

    • Author(s)
      Bizen N
    • Journal Title

      Stem Cells

      Volume: Epub ahead of print] Issue: 6 Pages: 1602-15

    • DOI

      10.1002/stem.1613

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Japanese Phaco-Goniosynechialysis Multicenter Study Group. Long-term efficacy of goniosynechialysis combined with phacoemulsification for primary angle closure2013

    • Author(s)
      Kameda T, Inoue T, Inatani M, Tanihara H
    • Journal Title

      Graefe's Archive for Clinical and Experimental Ophthalmology

      Volume: 251 Issue: 3 Pages: 825-830

    • DOI

      10.1007/s00417-012-2091-8

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Risk factors for hyphema after trabeculectomy with mitomycin C2013

    • Author(s)
      Kojima S, Inatani M, Shobayashi K, Haga, A, Inoue T, Tanihara H
    • Journal Title

      Journal of Glaucoma

      Volume: (in press) Issue: 5 Pages: 307-311

    • DOI

      10.1097/ijg.0b013e3182741c85

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Filtration bleb revision guided by three-dimensional anterior segment optical coherence tomography2013

    • Author(s)
      Kojima S, Inoue T, Kawaji T, Tanihara H
    • Journal Title

      Journal of Glaucoma

      Volume: (in press) Issue: 5 Pages: 312-315

    • DOI

      10.1097/ijg.0b013e3182741ee6

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Phase 1 Clinical Trials of a Selective Rho Kinase Inhibitor, K-115.2013

    • Author(s)
      Tanihara H
    • Journal Title

      JAMA Ophthalmology

      Volume: 印刷中

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] A prospective comparison between trabeculectomy with mitomycin C and phacotrabeculectomy with mitomycin C.2013

    • Author(s)
      Ogata-Iwao M
    • Journal Title

      Acta Ophthalmologica

      Volume: 印刷中

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Frequency and risk factors for neovascular glaucoma after vitrectomy in eyes with proliferative diabetic retinopathy.2013

    • Author(s)
      Goto A
    • Journal Title

      Journal of Glaucoma

      Volume: 印刷中

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] The effect of Rho-associated protein kinase inhibitor on monkey Schlemm's canal endothelial cells2012

    • Author(s)
      Kameda T, Inoue T, Inatani M, 9人3番目
    • Journal Title

      Invest Ophthalmol Vis Sci

      Volume: 53 Issue: 6 Pages: 3092-3103

    • DOI

      10.1167/iovs.11-8018

    • Related Report
      2013 Final Research Report 2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Simultaneous increases in multiple proinflammatory cytokines in the aqueous humor of pseudophakic glaucomatous eyes2012

    • Author(s)
      Inoue T, Kawaji T, Inatani M, Kameda T, Yoshimura N, Tanihara H
    • Journal Title

      Journal of Cataract and Refractive Surgery

      Volume: 38 Issue: 8 Pages: 1389-1397

    • DOI

      10.1016/j.jcrs.2012.04.028

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] The effect of monocyte chemoattractant protein-1/CC chemokine ligand 2 on aqueous humor outflow facility2012

    • Author(s)
      Tsuboi N, Inoue T, Kawai M, Inoue-Mochita M, Fujimoto T, Awai-Kasaoka N, Yoshida A, Tanihara H
    • Journal Title

      Investigative Ophthalmology & Visual Science

      Volume: 53 Issue: 10 Pages: 6702-6707

    • DOI

      10.1167/iovs.12-10376

    • Related Report
      2013 Final Research Report 2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Involvement of RhoA/Rho-associated kinase signal transduction pathway in dexamethasone-induced alterations in aqueous outflow.2012

    • Author(s)
      Fujimoto T
    • Journal Title

      Investigative Ophthalmology and Visual Science

      Volume: 53 Issue: 11 Pages: 7097-7108

    • DOI

      10.1167/iovs.12-9989

    • Related Report
      2013 Final Research Report 2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Elevated levels of monocyte chemoattractant protein-1 in the aqueous humor after phacoemulsification.2012

    • Author(s)
      Kawai M
    • Journal Title

      Investigative Ophthalmology and Visual Science

      Volume: 53 Issue: 13 Pages: 7951-7960

    • DOI

      10.1167/iovs.12-10231

    • Related Report
      2013 Final Research Report 2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Precise identification of filtration openings on the scleral flap by three-dimensional anterior segment optical coherence tomography2012

    • Author(s)
      Inoue T, Matsumura R, Kuroda U, Nakashima KI, Kawaji T, Tanihara H
    • Journal Title

      Investigative Ophthalmology & Visual Science

      Volume: 53 Issue: 13 Pages: 8288-8294

    • DOI

      10.1167/iovs.12-10941

    • Related Report
      2013 Final Research Report 2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Prognostic risk factors for failure of trabeculectomy with mitomycin C after vitrectomy2012

    • Author(s)
      Inoue T, Inatani M, Takihara Y, Awai-Kasaoka N, Ogata-Iwao M, Tanihara H
    • Journal Title

      Japanese Journal of Ophthalmology

      Volume: 56 Issue: 5 Pages: 1389-1397

    • DOI

      10.1007/s10384-012-0171-2

    • NAID

      10031039818

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] The effect of Rho-associated protein kinase inhibitor on monkey Schlemm’s canal endothelial cells.2012

    • Author(s)
      Kameda T, Inoue T, Inatani M, Fujimoto T, Honjo M, Kasaoka N, Inoue-Mochita M, Yoshimura N, Tanihara H.
    • Journal Title

      Investigative Ophthalmology & Visual Science.

      Volume: 印刷中

    • Related Report
      2011 Research-status Report
    • Peer Reviewed
  • [Journal Article] 緑内障セミナー 134. 房水流出路と細胞骨格.2011

    • Author(s)
      井上俊洋
    • Journal Title

      あたらしい眼科

      Volume: 28 Pages: 1135-1136

    • Related Report
      2011 Research-status Report
  • [Presentation] Identification of TGF-β2-induced proinflammatory cytokines secreted from cultured trabecular meshwork cells2013

    • Author(s)
      Inoue-Mochita M, Inoue T, Fujimoto T, Awai-Kasaoka N, Tanihara H
    • Organizer
      The Annual Meeting of the Association for Research in Vision and Ophthalmology
    • Place of Presentation
      Washington State Convention Center, Seattle, Washington, USA
    • Related Report
      2013 Final Research Report
  • [Presentation] Visualization of actin filament using time-lapse fluorescent microscopy in trabecular meshwork cells2013

    • Author(s)
      Fujimoto T, Inoue T, Inoue-Mochita M, Kasaoka N, Shobayashi K, Tanihara H
    • Organizer
      World Glaucoma Congress 2013
    • Place of Presentation
      Convention Centre, Vancouver, Canada
    • Related Report
      2013 Final Research Report
  • [Presentation] 線維柱帯細胞においてROCK阻害薬が酸化ストレスに与える影響2013

    • Author(s)
      笠岡奈々子,井上俊洋,亀田隆範,藤本智和,井上みゆき,谷原秀信
    • Organizer
      第117回日本眼科学会総会
    • Place of Presentation
      東京国際フォーラム
    • Related Report
      2013 Final Research Report
  • [Presentation] Investigation of Multiple Proinflammatory Cytokines in the Aqueous Humor in Eyes with Secondary Glaucoma2013

    • Author(s)
      Inoue T, Tanihara H
    • Organizer
      The Annual Meeting of the Association for Research in Vision and Ophthalmology
    • Place of Presentation
      Seattle, Washington, USA
    • Related Report
      2013 Annual Research Report
  • [Presentation] Short-term prospective investigation of filtering bleb by three dimensional anterior-segment optical coherence tomography2013

    • Author(s)
      Kojima S, Inoue T, Nakashima K, Fukushima A, Tanihara H
    • Organizer
      The Annual Meeting of the Association for Research in Vision and Ophthalmology
    • Place of Presentation
      Seattle, Washington, USA
    • Related Report
      2013 Annual Research Report
  • [Presentation] Identification of TGF-β2-induced proinflammatory cytokines secreted from cultured trabecular meshwork cells2013

    • Author(s)
      Inoue-Mochita M, Inoue T, Fujimoto T, Awai-Kasaoka N, Tanihara H
    • Organizer
      The Annual Meeting of the Association for Research in Vision and Ophthalmology
    • Place of Presentation
      Seattle, Washington, USA
    • Related Report
      2013 Annual Research Report
  • [Presentation] Evaluation of filtering blebs with transconjunctival oozing by anterior segment optical coherence tomography2013

    • Author(s)
      Nakashima K, Inoue T, Fukushima A, Hirakawa S, Kawaji T, Tanihara H
    • Organizer
      The Annual Meeting of the Association for Research in Vision and Ophthalmology
    • Place of Presentation
      Seattle, Washington, USA
    • Related Report
      2013 Annual Research Report
  • [Presentation] Investigation of Multiple Proinflammatory Cytokines in the Aqueous Humor in Eyes with Secondary Glaucoma2013

    • Author(s)
      Inoue T, Tanihara H
    • Organizer
      World Glaucoma Congress 2013
    • Place of Presentation
      Vancouver, Canada
    • Related Report
      2013 Annual Research Report
  • [Presentation] Short-term prospective investigation of filtering bleb by three dimensional anterior-segment optical coherence tomography2013

    • Author(s)
      Kojima S, Inoue T, Nakashima K, Fukushima A, Tanihara H
    • Organizer
      World Glaucoma Congress 2013
    • Place of Presentation
      Vancouver, Canada
    • Related Report
      2013 Annual Research Report
  • [Presentation] Visualization of actin filament using time-lapse fluorescent microscopy in trabecular meshwork cells2013

    • Author(s)
      Fujimoto T, Inoue T, Inoue-Mochita M, Kasaoka N, Shobayashi K, Tanihara H
    • Organizer
      World Glaucoma Congress 2013
    • Place of Presentation
      Vancouver, Canada
    • Related Report
      2013 Annual Research Report
  • [Presentation] The effects of Rho/ROCK activation in dexamethasone-induced increase of aqueous-outflow resistance2012

    • Author(s)
      Fujimoto T, Inoue T, Inoue-Mochita M, Kameda T, Kasaoka N, Tanihara H
    • Organizer
      The Annual Meeting of the Association for Research in Vision and Ophthalmology
    • Place of Presentation
      Convention Center, Fort Lauderdale, Florida, USA
    • Related Report
      2013 Final Research Report
  • [Presentation] The effect of monocyte chemoattractant protein-1/CC chemokine ligand 2 on the aqueous humor outflow facility2012

    • Author(s)
      Tsuboi N, Inoue T, Fujimoto T, Inoue-Mochita M, Kasaoka N, Kawai M, Shobayashi K, Yoshida A, Tanihara H
    • Organizer
      The Annual Meeting of the Association for Research in Vision and Ophthalmology
    • Place of Presentation
      Convention Center, Fort Lauderdale, Florida, USA
    • Related Report
      2013 Final Research Report
  • [Presentation] Involvement of RhoA/Rho-associated kinase signal transduction pathway in the dexamethasone-induced alterations in aqueous outflow2012

    • Author(s)
      Fujimoto T, Inoue T, Inoue-Mochita M, Kameda T, Kasaoka N, Tanihara H
    • Organizer
      European Glaucoma Society 2012
    • Place of Presentation
      Tivoli Hotel & Congress Center, Copenhagen, Denmark
    • Related Report
      2013 Final Research Report
  • [Presentation] The effect of monocyte chemoattractant protein-1/CC chemokine ligand 2 on the aqueous humor outflow facility2012

    • Author(s)
      Tsuboi N, Inoue T, Fujimoto T, Inoue-Mochita M, Kasaoka N, Kawai M, Shobayashi K, Yoshida A, Tanihara H
    • Organizer
      European Glaucoma Society 2012
    • Place of Presentation
      Tivoli Hotel & Congress Center, Copenhagen, Denmark
    • Related Report
      2013 Final Research Report
  • [Presentation] Filtration bleb revision guided by three-dimensional anterior segmental optical coherence tomography.2012

    • Author(s)
      Kojima S
    • Organizer
      The 27th APAO Congress
    • Place of Presentation
      Pusan, Korea
    • Related Report
      2012 Research-status Report
  • [Presentation] Multiplex Immunoassay of Aqueous Humor in Cataract Patients: Impact of Phacoemulsification.2012

    • Author(s)
      Kawai M
    • Organizer
      The Annual Meeting of the Association for Research in Vision and Ophthalmology
    • Place of Presentation
      Fort Lauderdale, Florida, USA
    • Related Report
      2012 Research-status Report
  • [Presentation] Pinpoint Identification of Filtration Opening on the Scleral Flap Margins Created by Trabeculectomy.2012

    • Author(s)
      Tanihara H
    • Organizer
      The Annual Meeting of the Association for Research in Vision and Ophthalmology
    • Place of Presentation
      Fort Lauderdale, Florida, USA
    • Related Report
      2012 Research-status Report
  • [Presentation] The Effects of Rho/ROCK Activation in Dexamethasone-Induced Increase of Aqueous-Outflow Resistance.2012

    • Author(s)
      Fujimoto T
    • Organizer
      The Annual Meeting of the Association for Research in Vision and Ophthalmology
    • Place of Presentation
      Fort Lauderdale, Florida, USA
    • Related Report
      2012 Research-status Report
  • [Presentation] The Effect of Monocyte Chemoattractant Protein-1/CC Chemokine Ligand 2 on The Aqueous Humor Outflow Facility2012

    • Author(s)
      Tsuboi N
    • Organizer
      The Annual Meeting of the Association for Research in Vision and Ophthalmology
    • Place of Presentation
      Fort Lauderdale, Florida, USA
    • Related Report
      2012 Research-status Report
  • [Presentation] A Prospective Study of Phakic vs Pseudophakic Eyes After Phacoemulsification in Trabeculectomy for Open-Angle Glaucoma.2012

    • Author(s)
      Takihara Y
    • Organizer
      The Annual Meeting of the Association for Research in Vision and Ophthalmology
    • Place of Presentation
      Fort Lauderdale, Florida, USA
    • Related Report
      2012 Research-status Report
  • [Presentation] Filtration Bleb-associated Tear Film Sign Demonstrated by Three Dimensional Anterior Segment Optical Coherence Tomography (3D AS-OCT).2012

    • Author(s)
      Kojima S
    • Organizer
      The Annual Meeting of the Association for Research in Vision and Ophthalmology
    • Place of Presentation
      Fort Lauderdale, Florida, USA
    • Related Report
      2012 Research-status Report
  • [Presentation] The effects of Rho/ROCK activation in dexamethasone induced increase of aqueous outflow resistance2011

    • Author(s)
      Fujimoto T, Inoue T, Inoue-Mochita M, Kameda T, Kasaoka N, Tanihara H
    • Organizer
      World Glaucoma Congress 2011
    • Place of Presentation
      Palais des Congrès, Paris, France
    • Related Report
      2013 Final Research Report
  • [Presentation] The Effect of Aqueous Monocyte Chemotactic Protein-1 Concentration on the Short-term Failure of Trabeculectomy with Mitomycin C in Eyes with Open-angle Glaucoma.2011

    • Author(s)
      Inoue T, Inatani M, Kameda T, Yoshimura N, Tanihara H.
    • Organizer
      World Glaucoma Congress 2011
    • Place of Presentation
      Palais des Congres, Paris, France
    • Related Report
      2011 Research-status Report
  • [Remarks] 熊本大学大学院生命科学研究部眼科学分野 研究業績

    • URL

      http://www2.kuh.kumamoto-u.ac.jp/ganka/kyousitu/gyouseki.html

    • Related Report
      2013 Annual Research Report
  • [Remarks] 熊本大学大学院生命科学研究部眼科学分野 研究業績

    • URL

      http://www2.kuh.kumamoto-u.ac.jp/ganka/kyousitu/gyouseki.html

    • Related Report
      2012 Research-status Report

URL: 

Published: 2011-08-05   Modified: 2019-07-29  

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