Comprehensive investigation of tension-sensitive factors that related to changes in cytoskeleton and cell adhesion in trabecular meshwork cells
| Project/Area Number |
23791994
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Ophthalmology
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| Research Institution | Kumamoto University |
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Principal Investigator |
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| Project Period (FY) |
2011 – 2013
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2013: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2011: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Keywords | 緑内障 / 線維柱帯 / 酸化ストレス / 圧感受性 / 細胞骨格 / 房水 / 線維柱体 / シュレム管内皮 |
|---|
| Research Abstract |
We made a device to add hydrostatic pressure on cultured cells, resulting in changes in cell morphology even in the control condition, and the data were not analyzable. After treatment of trabecular meshwork cells with Y-27632, 5 genes that were related to antioxidant functions were up-regulated. Of those, the up-regulation of catalase was confirmed by realtime RT-PCR. Pretreatment with Y-27632 increased the signal of WST-8 assay and decreased the signal of DCFDA test reagent under stimulation with hydrogen peroxide. Collectively, it was indicated that ROCK inhibitor induced intracellular expression of antioxidants in the trabecular meshwork cells, and thereby suppressed oxidative stress-induced cell death.
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Report
(4 results)
Research Products
(52 results)
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[Journal Article] The effect of Rho-associated kinase inhibition on the ocular penetration of timolol maleate2013
Author(s)
Arnold JJ, Hansen MS, Gorman GS, Inoue T, Rao V, Spellen S, Hunsinger RN, Chapleau CA, Pozzo-Miller L, Stamer WD, Challa P
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Journal Title
Investigative Ophthalmology and Visual Science
Volume: 54
Issue: 2
Pages: 1118-1126
DOI
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Peer Reviewed
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[Presentation] The effect of monocyte chemoattractant protein-1/CC chemokine ligand 2 on the aqueous humor outflow facility2012
Author(s)
Tsuboi N, Inoue T, Fujimoto T, Inoue-Mochita M, Kasaoka N, Kawai M, Shobayashi K, Yoshida A, Tanihara H
Organizer
The Annual Meeting of the Association for Research in Vision and Ophthalmology
Place of Presentation
Convention Center, Fort Lauderdale, Florida, USA
Related Report
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[Presentation] The effect of monocyte chemoattractant protein-1/CC chemokine ligand 2 on the aqueous humor outflow facility2012
Author(s)
Tsuboi N, Inoue T, Fujimoto T, Inoue-Mochita M, Kasaoka N, Kawai M, Shobayashi K, Yoshida A, Tanihara H
Organizer
European Glaucoma Society 2012
Place of Presentation
Tivoli Hotel & Congress Center, Copenhagen, Denmark
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