Development of new therapy for corneal endothelial dysfunction by modulating epithelial-mesenchymal transition
| Project/Area Number |
23791998
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Ophthalmology
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| Research Institution | Doshisha University |
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Principal Investigator |
OKUMURA Naoki 同志社大学, 生命医科学部, 助教 (10581499)
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| Project Period (FY) |
2011 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2011: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
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| Keywords | 角膜移植 / 角膜内皮細胞 / Rhoキナーゼ阻害剤 / アポトーシス / 角膜内皮 / 上皮間葉移行 |
|---|
| Research Abstract |
We established an optimum condition for the cultivation of HCECs. When exposed to culture conditions, both primate and human CECs showed two distinct phenotypes; contact-inhibited polygonal monolayer and fibroblastic phenotypes. The use of SB431542, a selective inhibitor of the transforming growth factor-beta (TGF-β) receptor, counteracted the fibroblastic phenotypes to the normal contact-inhibited monolayer, and these polygonal cells maintained endothelial physiological functions.
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Report
(3 results)
Research Products
(84 results)
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[Presentation] ROCK Inhibition Regulates the Cell Adhesion of Corneal Endothelial Cells2011
Author(s)
Kyoko Kumagai, Noriko Koizumi, Naoki Okumura, Kenta Yamazaki, Morio Ueno, Yuji Sakamoto, Nagahisa Yoshimura1, Junji Hamuro, Shigeru Kinoshita
Organizer
Annual Meeting of the Association for Research in Vision and Ophthalmology
Place of Presentation
Fort Lauderdale, Florida, USA
Related Report
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[Presentation] Cell-Injection Therapy using Cultivated Corneal Endothelial Cells Combined with a ROCK Inhibitor in a Primate Model2011
Author(s)
Noriko Koizumi, Naoki Okumura, Kenta Yamasaki, Morio Ueno, Yuji Sakamoto, Hiroaki Takahashi, Ryuzo Torii, Junji Hamuro, Shigeru Kinoshita
Organizer
Annual Meeting of the Association for Research in Vision and Ophthalmology
Place of Presentation
Fort Lauderdale,Florida, USA
Related Report
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