Cyclosporin A inhibits cell death of cornel endothelial cells by protecting mitochondrial membrane potential
| Project/Area Number |
23792005
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Ophthalmology
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| Research Institution | Juntendo University |
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Principal Investigator |
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| Project Period (FY) |
2011 – 2013
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2013: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2012: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2011: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
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| Keywords | 角膜内皮細胞 / 角膜移植 / 細胞死 / シクロスポリン / ミトコンドリア / 角膜移植術 / 膜電位 |
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| Research Abstract |
We corfirmed H2O2 induced a dose dependent increase in cell death of cultured human corneal endotehelial cells(HCECs). At concentration of 100nM, Cyclosporine A(CsA) attenuated the H2O2-induced cell death of HCECs by 15%.CsA significantly inhibited the permiability trasition pore(PTP) in H2O2 treaed HCECs. The mitochondrial membrane potential of H2O2 treated mouse corneal cup were protected by CsA at concetration of 100nM.
Cyclosporine A protected mitochondrial menmbrane potential from oxidative stress, abd attenuated cell death of corneal endothelial cells.
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Report
(4 results)
Research Products
(5 results)
