gene therapy using siena technology
| Project/Area Number |
23792014
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Ophthalmology
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| Research Institution | Nippon Medical School |
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Principal Investigator |
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| Project Period (FY) |
2011 – 2013
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2012: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2011: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Keywords | 網膜 / 遺伝子治療 / AAVベクター / siRNA / VEGF / 脈絡膜新生血管 / 血管新生 / 眼科 / 新生血管 / 抑制 / 遺伝子 / ウィルスベクター |
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| Research Abstract |
To assess the feasibility of a gene therapeutic approach to treating choroidal neovascularization (CNV), we generated an adeno-associated virus type 8 vector (AAV8) encoding a small interfering RNA (siRNA) targeting vascular endothelial growth factor (VEGF), and determined its ability to inhibit angiogenesis. We made AAV8/SmVEGF-2. We injected AAV8/SmVEGF-2 or control vector (AAV8/GFP) into the subretinal space in C57BL/6 mice. The eyes were removed for flat mount analysis of CNV surface area. Subretinal delivery of AAV8/SmVEGF-2 significantly diminished CNV at the laser lesions, as compared to AAV8/GFP Using ELISA, we found that VEGF levels were reduced by approximately half in AAV8/SmVEGF-2 treated eyes. These results suggest that siRNA-VEGF can be expressed across the retina and that long-term suppression of CNV is possible through the use of stable AAV-mediated siRNA-VEGF expression.
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Report
(4 results)
Research Products
(45 results)
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[Journal Article] Rapid and Transient Upregulation of CCL11 (Eotaxin-1) in Mouse Ovary During Terminal Stages of Follicular Development2012
Author(s)
Kuwabara Y, Katayama A, Igarashi T, Tomiyama R, Piao H, Kaneko R, Abe T, Mine K, Akira S, Orimo H, Takeshita T
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Journal Title
Am J Reprod Immunol
Volume: 67(5)
Pages: 358-368
Related Report
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[Journal Article] A new mutation in RP1L1 gene in occult macular dystrophy patient associated with a depolarizing-pattern of focal macular ERG2012
Author(s)
Kabuto T, Takahashi H, Goto-Fukuura Y, Igarashi T, Akahori M, Kameya S, Iwata T, Mizota A, Yamaki K, Miyake Y, Takahashi H
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Journal Title
Molecular Vision
Volume: 18
Pages: 1031-1039
Related Report
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[Journal Article] Rapid and Transient Upregulation of CCL11 (Eotaxin-1) in Mouse Ovary During Terminal Stages of Follicular Development.2012
Author(s)
Kuwabara Y, Katayama A, Igarashi T, Tomiyama R, Piao H, Kaneko R, Abe T, Mine K, Akira S, Orimo H, Takeshita T.
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Journal Title
Am J Reprod Immunol.
Volume: 67
Pages: 358-68
Related Report
Peer Reviewed
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[Journal Article] A new mutation in RP1L1 gene in occult macular dystrophy patient associated with a depolarizing-pattern of focal macular ERG.2012
Author(s)
Kabuto T, Takahashi H, Goto-Fukuura Y, Igarashi T, Akahori M, Kameya S, Iwata T, Mizota A, Yamaki K, Miyake Y, Takahashi H.
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Journal Title
Molecular Vision.
Volume: 18
Pages: 1031-9
Related Report
Peer Reviewed
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