Dependence of corneal stem/progenitor cells on ocular surface innervation.

• Project/Area Number: 23792015
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Ophthalmology
• Research Institution: St. Marianna University School of Medicine
• Principal Investigator: UENO Hiroki 聖マリアンナ医科大学, 医学部, 助教 (30529897)
• Project Period (FY): 2011 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2012: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000); Fiscal Year 2011: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: 角膜 / 三叉神経 / 角膜 stem/progenitor 細胞 / 角膜三叉神経 / 角膜stem/progenitor細胞 / stem/progenitor 細胞 / 神経麻痺性角膜炎

Research Abstract

Trigeminal stereotactic electrolysis (TSE) was able to induce a disease state that reflected clinical neurotrophic keratitis. Moreover, corneolimbal stem/progenitor cells are significantly reduced after depletion of sensory nerves. The datasuggest a critical role of innervation in maintaining stem cells and/or the stem cell niche.

Research Products

• [Journal Article] Nerves and Neovessels Inhibit Each Other in the Cornea. (2013)
  • Author(s): Ferrari G, Ueno H, Dana R
  • Journal Title: Invest Ophthalmol Vis Sci. Volume: 54 Issue: 1 Pages: 813-820
  • DOI: 10.1167/iovs.11-8379
  • Peer Reviewed
• [Journal Article] Trigeminal stereotactic electrolysis induces dry eye in mice. (2013)
  • Author(s): Ferrari G,Ueno H, Dana R.
  • Journal Title: Acta Ophthalmologica. Volume: 91 Issue: 2 Pages: 162-163
  • DOI: 10.1111/j.1755-3768.2012.02529.x
  • Peer Reviewed
• [Journal Article] Donor-derived, tolerogenic dendritic cells suppress immune rejection in the indirect allosensitization-dominant setting of corneal transplantation (2012)
  • Author(s): Takaaki Hattori, Daniel R Saban, Emami-Naeini P, Sunil K. Chauhan, Toshinari Funaki, Hiroki Ueno, Reza Dana
  • Journal Title: J Leukoc Biol. Volume: 91 Issue: 4 Pages: 621-627
  • DOI: 10.1189/jlb.1011500
  • Peer Reviewed
• [Journal Article] Imaging of a severe case of acute hydrops in a patient with keratoconus using anterior segment optical coherence tomography. (2012)
  • Author(s): Ueno H, Matuzawa A, Kumagai Y, Takagi H, Ueno S
  • Journal Title: Case Rep Ophthalmol. Volume: 3 Issue: 3 Pages: 304-310
  • DOI: 10.1159/000337483
  • Peer Reviewed
• [Journal Article] Dependence of corneal stem/progenitor cells on ocular surface innervation (2012)
  • Author(s): Hiroki Ueno, Ferrari Giulio Ferrari, Takaaki Hattori, Daniel R Saban, Katikireddy KR, Sunil K. Chauhan, Reza Dana.
  • Journal Title: Invest Ophthalmol Vis Sci. Volume: 53 Issue: 2 Pages: 867-872
  • DOI: 10.1167/iovs.11-8438
  • Peer Reviewed
• [Journal Article] Donor-derived, tolerogenic dendritic cells suppress immune rejection in the indrect allosensitization-dominant setting of corneal transplantation (2012)
  • Author(s): Hattori T, Ueno H, Dana R.
  • Journal Title: J Leukoc Biol. Volume: 91 (4) Pages: 621-627
  • Peer Reviewed
• [Journal Article] Imaging of a severe case of acute hydrops in a patient with keratoconus (2012)
  • Author(s): Ueno H, Kumagai Y, Ueno S.
  • Journal Title: Case Reports in Ophthalmology Volume: in press
  • Peer Reviewed
• [Journal Article] Dependence of stem cells on ocular innervation. (2012)
  • Author(s): Ueno H, Ferrari G, Hattori T, Dana R.
  • Journal Title: Invest Ophthalmol Vis Sci. Volume: 53 (2) Pages: 867-872
  • Peer Reviewed
• [Journal Article] Characterization of Langerin- expressing dendritic cell subsets in the normal cornea (2011)
  • Author(s): Takaaki Hattori, Suni K. Chauhan, Hyunsoo Lee, Hiroki Ueno, Reza Dana, Kaplan DH, Daniel R Saban.
  • Journal Title: Invest Ophthalmol Vis Sci. Volume: 52 Issue: 7 Pages: 4598-4604
  • DOI: 10.1167/iovs.10-6741
  • Peer Reviewed
• [Journal Article] A Novel Mouse Model for Neurotrophic Keratopathy: Trigeminal Nerve Stereotactic Electrolysis through the Brain. (2011)
  • Author(s): Giulio Ferrari, Sunil K. Chauhan, Hiroki Ueno, Nambi Nallasamy, Stefano Gandolfi, Lawrence Borges, Reza Dana.
  • Journal Title: Invest Ophthalmol Vis Sci Volume: 52 Issue: 5 Pages: 2532-2539
  • DOI: 10.1167/iovs.10-5688
  • Peer Reviewed
• [Journal Article] Characterization of Langerin-expressing dendritic cell subsets in the normal cornea. (2011)
  • Author(s): Hattori T, Ueno H, Saban DR.
  • Journal Title: Invest Ophthalmol Vis Sci. Volume: 52 (7) Pages: 4598-4604
  • Peer Reviewed
• [Journal Article] Induction of Corneal Epithelium-Like Cells From Cynomolgus Monkey Embryonic Stem Cells and Their Experimental Transplantation to Damaged Cornea (2010)
  • Author(s): Yuta Kumagai, Manae S. Kurokawa, Hiroki Ueno, Maki Kayama, Kazuo Tsubota, Norio Nakatsuji, Shinji Nito, Satoki Ueno, Noboru Suzuki
  • Journal Title: Cornea Volume: 29 Issue: 4 Pages: 432-438
  • DOI: 10.1097/ico.0b013e3181b9ffcc
  • Peer Reviewed
• [Journal Article] Transfection with pax6 gene of mouse embryonic stem cells and subsequent cell cloning induced retinal neuron progenitors, including retinal ganglion cell-like cells, in vitro (2010)
  • Author(s): Maki Kayama, Manae S. Kurokawa, Yuji Ueda, Hiroki Ueno, Yuta Kumagai, Shunmei Chiba, Erika Takada, Satoki Ueno, Mamoru Tadokoro and Noboru Suzuki.
  • Journal Title: Ophthalmic Res. Volume: 43(2) Issue: 2 Pages: 79-91
  • DOI: 10.1159/000247592
  • Peer Reviewed
• [Presentation] 角膜stem/progenitorcellと角膜神経との相互作用について (2013)
  • Author(s): 上野宏樹
  • Organizer: 角膜カンファランス2013シンポジウム
  • Place of Presentation: 白浜
  • Year and Date: 2013-02-15
• [Presentation] 3者併用療法に希釈ポピドンヨード液を治療に用いたアカントアメーバ角膜炎の3症例 (2012)
  • Author(s): 上野宏樹、松澤亜紀子、熊谷悠太、高木均、上野聰樹
  • Organizer: 第66回日本臨床眼科学会
  • Place of Presentation: 京都
  • Year and Date: 2012-10-27
• [Presentation] EvaluationofCornealProgenitorCellsinMicewithTypeIIDiabetesMellitus (2012)
  • Author(s): UenoH,HattoriT,KumagaiY,TakadaE,SuzukiN,UenoS.
  • Organizer: TheAssociationforResearchinvisionandOphthalmology
  • Place of Presentation: FortLauderdale,USA
  • Year and Date: 2012-05-08
• [Presentation] 重症円錐角膜急性水腫後前眼部OCTにて長期経過を観察した1例 (2012)
  • Author(s): 上野宏樹、松澤亜紀子、熊谷悠太、高木均、上野聰樹.
  • Organizer: 角膜カンファランス2012
  • Place of Presentation: 東京
  • Year and Date: 2012-02-23
• [Presentation] 角膜stem/progenitorcellと角膜神経との相互作用について (2012)
  • Author(s): 上野宏樹.
  • Organizer: 第16回眼科分子生物学会
  • Place of Presentation: 山鹿
  • Year and Date: 2012-02-11
• [Presentation] 高眼圧により視神経変化を伴ったIgG4関連ミクリッツ病の1例 (2011)
  • Author(s): 上野宏樹、熊谷悠太、阿藤晃一、相原正記、高木均、上野聰樹.
  • Organizer: 第65回日本臨床眼科学
  • Place of Presentation: 東京
  • Year and Date: 2011-10-07
• [Presentation] EvaluationofCornealProgenitorCellsinaMurineModelofNeurotrophicKeratopathy (2011)
  • Author(s): UenoH,FerrariG,HattoriT,UenoS,ChauhanS,DanaR.
  • Organizer: TheAssociationforResearchinvisionandOphthalmology
  • Place of Presentation: FortLauderdale,USA
  • Year and Date: 2011-05-04
• [Presentation] Evaluation of Corneal Progenitor Cells in a Murine Model of Neurotrophic keratopathy (2011)
  • Author(s): Ueno H et al.
  • Organizer: The Association for Research in Vision and Ophthalmology
  • Place of Presentation: Fort Lauderdale
• [Presentation] NerveRegenerationAssociateWithStemCellEnvironment (2010)
  • Author(s): UenoH,FerrariG,HattoriT,FunakiT,SabanD,ChauhanS,DanaR
  • Organizer: TheAssociationforResearchinvisionandOphthalmology
  • Place of Presentation: FortLauderdale,USA
  • Year and Date: 2010-05-03
• [Presentation] NerveRegenerationDependsonStemCellEnvironment (2010)
  • Author(s): UenoH,FerrariG,HattoriT,FunakiT,SabanD,ChauhanS,DanaR.
  • Organizer: TheWorldCorneaCongressVII(WCCVII)
  • Place of Presentation: Boston,USA
  • Year and Date: 2010-04-08
• [Presentation] Evaluation of Corneal Progenitor Cells in Mice with Type II Diabetes Mellitus
  • Author(s): H. Ueno
  • Organizer: The Association for Research in Vision and Ophthalmology
  • Place of Presentation: United States: Fort Lauderdale
• [Presentation] 角膜神経障害での幹細胞の影響
  • Author(s): 上野宏樹
  • Organizer: 角膜カンファランス2013
  • Place of Presentation: 和歌山
  • Invited