Roles of soluble VEGF receptor on angiogenesis of eye in vivo

• Project/Area Number: 23792017
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Ophthalmology
• Research Institution: Kanazawa Medical University
• Principal Investigator: IKEDA Takayuki 金沢医科大学, 医学部, 講師 (00374942)
• Research Collaborator: HATTA Toshihisa 金沢医科大学, 医学部, 教授 (20238025)
• Project Period (FY): 2011 – 2013
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2013: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000); Fiscal Year 2012: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000); Fiscal Year 2011: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
• Keywords: 可溶型Flt-1 / HMVEC / RNA結合タンパク質 / レンチウイルス / MHVEC / 選択的3'端プロセシング / 血管形成

Research Abstract

Here, we showed the mechanisms for regulating alternative 3'-end processing of soluble Flt-1, a potential antiangiogenic factor. We found that hnRNP D down-regulated soluble Flt-1 expression by binding to the regulatory sequence on intron 13 of Flt-1 pre-mRNA. Furthermore, we carried out the lentiviral mediated transgenesis by in vivo manipulation of spermatogonical stem cell. It was suggested that the method was not effective because lentiviral particle couldn't infect to spermatogonical stem cell. Therefore, we are going to create a transgenic mouse conventionally and show the roles of soluble Flt-1 on angiogenesis of eye in vivo.

Research Products

• [Journal Article] Identification of a major enzyme for the synthesis and hydrolysis of cyclic ADP-ribose in amphibian cells and evolutional conservation of the enzyme from human to invertebrate (2012)
  • Author(s): Ikeda T, Takasawa S, Noguchi N, Nata K, Yamauchi A, Takahashi I, Yoshikawa T, Sugawara A, Yonekura H, Okamoto H.
  • Journal Title: Mol Cell Biochem Volume: 366 Issue: 1-2 Pages: 69-80
  • DOI: 10.1007/s11010-012-1284-0
  • Peer Reviewed
• [Journal Article] Hypoxia down-regulates sFlt-1 (sVEGFR-1) expression in human microvascular endothelial cells by a mechanism involving mRNA alternative processing (2011)
  • Author(s): Ikeda T, Sun L, Tsuruoka N, Ishigaki Y, Yoshitomi Y, Yoshitake Y, Yonekura H
  • Journal Title: Biochem. J Volume: 436(2) Issue: 2 Pages: 399-407
  • DOI: 10.1042/bj20101490
  • Peer Reviewed
• [Journal Article] Identification of the COL2A1 Mutation in Patients with Type I Stickler Syndrome Using RNA from Freshly Isolated Peripheral White Blood Cells (2011)
  • Author(s): Yaguchi H, Ikeda T, Osada H, Yoshitake Y, Sasaki H, Yonekura H
  • Journal Title: Genet. Test. Mol. Biomarkers Volume: 15(4) Issue: 4 Pages: 231-237
  • DOI: 10.1089/gtmb.2010.0138
  • Peer Reviewed
• [Journal Article] Induction of HITS, a newly identified family with sequence similarity 107 protein(FAM107B), in cancer cells by heat shock stimulation (2010)
  • Author(s): H. Nakajima, Y. Ishigaki, QS. Xia, T. Ikeda, Y. Yoshitake, H. Yonekura, T. Nojima, T. Tanaka, H. Umehara, N. Tomosugi, T. Takata, T. Shimasaki, N. Nakaya, I. sato, K. Kawakami, K. Koizumi, T. Minamoto, Y. Motoi
  • Journal Title: Int. J. Oncol Volume: 37 Issue: 3 Pages: 583-593
  • DOI: 10.3892/ijo_00000707
  • Peer Reviewed
• [Presentation] 神経—血管相互作用を介した血管ネットワーク形成におけるJunBの機能 (2013)
  • Author(s): 吉冨泰央、池田崇之、吉竹佳の、八田稔久、加藤伸郎、米倉秀人
  • Organizer: 金沢医科大学医学会第47回学術集会
  • Place of Presentation: 石川県内灘
  • Year and Date: 2013-07-06
• [Presentation] 転移能の異なるルイス肺がん細胞株が形成する腫瘍中に誘導される血管の構造の際の解明 (2013)
  • Author(s): 吉冨泰央、池田崇之、吉竹佳の、岡山 實、小栗佳代子、米倉秀人
  • Organizer: 第86回 日本生化学会
  • Place of Presentation: 神奈川県横浜市 パシフィコ横浜
• [Presentation] 神経ー血管相互作用を介した血管ネットワーク形成におけるJunBの機能 (2013)
  • Author(s): 吉冨泰央、池田崇之、吉竹佳の、八田稔久、加藤伸郎、米倉秀人
  • Organizer: 金沢医科大学医学会 第４９回学術集会
  • Place of Presentation: 石川県内灘町 金沢医科大学
• [Presentation] 毛細血管内皮細胞のマトリゲル上での管腔形成過程ではEpac2の発現が上昇する (2012)
  • Author(s): 吉竹佳の、池田崇之、吉冨泰央、田中一美、米倉秀人
  • Organizer: 第85回日本生化学会大会
  • Place of Presentation: 福岡
  • Year and Date: 2012-12-16
• [Presentation] Flt-1(VEGF受容体-1)mRNA選択的3'端プロセシング機構の解析 (2012)
  • Author(s): 池田崇之、吉冨泰央、吉竹佳の、米倉秀人
  • Organizer: 第85回日本生化学会大会
  • Place of Presentation: 福岡
  • Year and Date: 2012-12-15
• [Presentation] 低酸素状態はmRNA選択的3'端プロセシングを介して微小血管内皮細胞の可溶型VEGF受容体(可溶型Flt-1)産生を抑制する (2012)
  • Author(s): 池田崇之、吉冨泰央、石垣靖人、吉竹佳の、米倉秀人
  • Organizer: 日本生化学会北陸支部第30回記念大会
  • Place of Presentation: 金沢
  • Year and Date: 2012-05-26
• [Presentation] Increased expression of Epac2 during in vitro tube formation in human microvascular endothelial cells (2012)
  • Author(s): Yoshitake, Y., Ikeda, T., Yoshitomi, Y. and Yonekura, H.
  • Organizer: 37th FEBS Congress
  • Place of Presentation: Seville, Spain
• [Presentation] 血管内皮細胞における可溶型Flt-1(可溶型VEGF受容体-1)mRNA選択的3'端プロセシング機構の解析 (2012)
  • Author(s): 池田崇之、吉冨泰央、石垣靖人、吉竹佳の、米倉秀人
  • Organizer: 第14回日本RNA学会
  • Place of Presentation: 仙台
• [Presentation] Ultraviolet B-induced expression of amphiregulin and growth differentiation factor 15 in human lens epithelial cells (2012)
  • Author(s): Osada, H., Yoshitake, Y., Ikeda, T., Ishigaki, Y., Takata, T., Tomosugi, N., Kubo, E., Sasaki, H. and Yonekura, H.
  • Organizer: International Conference on The Lens
  • Place of Presentation: Hawaii
• [Presentation] Flt-1（VEGF受容体-1）mRNA選択的3’端プロセシング機構の解析 (2012)
  • Author(s): 池田崇之
  • Organizer: 第85回日本生化学会大会
  • Place of Presentation: マリンメッセ福岡（福岡市）
• [Presentation] 毛細血管内皮細胞のマトリゲル上での管腔形成過程ではEpac2の発現が上昇する (2012)
  • Author(s): 吉竹 佳乃
  • Organizer: 第85回日本生化学会大会
  • Place of Presentation: マリンメッセ福岡（福岡市）
• [Presentation] 血管内皮細胞における可溶型Flt-1（可溶型VEGF受容体-1）mRNA選択的3’端プロセシング機構の解析 (2012)
  • Author(s): 池田崇之
  • Organizer: 第14回日本RNA学会年会
  • Place of Presentation: 川内萩ホール（仙台市）
• [Presentation] Increased expression of Epac2 during in vitro tube formation in human microvascular endothelial cells. (2012)
  • Author(s): Yoshitake Y
  • Organizer: 37th FEBS Congress
  • Place of Presentation: Sevilla (Spain)
• [Presentation] 低酸素刺激による微小血管内皮細胞の可溶型VEGF受容体(可溶型Flt-1)産生の抑制 (2011)
  • Author(s): 池田崇之、吉冨泰央、石垣靖人、吉竹佳の、米倉秀人
  • Organizer: RNAフロンティアミーティング
  • Place of Presentation: 愛知県大府
  • Year and Date: 2011-08-31
• [Presentation] 低酸素刺激による微小血管内皮細胞の可溶型VEGF受容体(可溶型Flt-1)産生の抑制 (2011)
  • Author(s): 池田崇之、吉冨泰央、石垣靖人、吉竹佳の、米倉秀人
  • Organizer: 金沢医科大学医学会第47回学術集会
  • Place of Presentation: 石川県内灘
  • Year and Date: 2011-07-09
• [Presentation] 低酸素刺激による微小血管内皮細胞の可溶型VEGF受容体(可溶型Flt-1)産生の抑制 (2011)
  • Author(s): 池田崇之
  • Organizer: 日本生化学会北陸支部第29回大会
  • Place of Presentation: 金沢
  • Year and Date: 2011-05-28
• [Presentation] Soluble VEGFR-1 production is down-regulated under hypoxia in human microvascular endothelial cells by a mechanism involving alternative mRNA 3′-end processing (2011)
  • Author(s): Ikeda, T., Yoshitomi, Y., Ishigaki, Y., Yoshitake, Y. and Yonekura, H.
  • Organizer: The Molecular Biology Society of Japan The 11th Spring Symposium International Symposium on Tumor Biology in Kanazawa
  • Place of Presentation: 金沢
  • Year and Date: 2011-05-25
• [Presentation] 神経—血管相互作用により血管で活性化される遺伝子群の同定とその機能解析 (2011)
  • Author(s): 吉冨泰央、池田崇之、吉竹佳の、加藤伸郎、米倉秀人
  • Organizer: 第84回日本生化学会大会
  • Place of Presentation: 京都
• [Presentation] Hypoxia doun-regulates sFlt-1 (sVEGFR-1) expression in human microvascular endothelial cells by a mechanism involving mRNA alternative processing (2011)
  • Author(s): Ikeda, T., Yoshitomi, Y., Ishigaki, Y., Yoshitake, Y. and Yonekura, H.
  • Organizer: 36th FEBS Congress
  • Place of Presentation: Torino, Italy
• [Presentation] Ultraviolet B-induced expression of amphiregulin and growth differentiation factor 15 in human lens epithelial cells (2011)
  • Author(s): Yoshitake, Y., Osada, H., Ikeda, T., Yoshitomi, Y., Ishigaki, Y., Sasaki, H. and Yonekura, H.
  • Organizer: 36th FEBS Congress
  • Place of Presentation: Torino, Italy
• [Presentation] 低酸素刺激による微小血管内皮細胞の可溶型VEGF受容体（可溶型Flt-1）産生の抑制 (2011)
  • Author(s): 池田崇之
  • Organizer: 日本生化学会北陸支部第29回大会
  • Place of Presentation: 金沢大学角間キャンパス（金沢市）
• [Presentation] Hypoxia down-regulates sFlt-1 (sVEGFR-1) expression in human microvascular endothelial cells by a mechanism involving mRNA alternative processing (2011)
  • Author(s): Ikeda T
  • Organizer: 36th FEBS CONGRESS
  • Place of Presentation: Lingotto Conference Center（Torino, Italy）
• [Presentation] Soluble VEGFR-1 production is down-regulated under hypoxia in human microvascular endothelial cells by a mechanism involving alternative mRNA 3′-end processing (2011)
  • Author(s): Ikeda T
  • Organizer: 日本分子生物学会第11回春季シンポジウム・金沢国際がん生物学シンポジウム
  • Place of Presentation: 石川県立音楽堂（金沢市）
• [Presentation] 低酸素刺激による微小血管内皮細胞の可溶型VEGF受容体（可溶型Flt-1）産生の抑制 (2011)
  • Author(s): 池田崇之
  • Organizer: RNAフロンティアミーティング2011
  • Place of Presentation: あいち健康プラザ（愛知県大府市）
• [Presentation] Ultraviolet B-induced expression of amphiregulin and growth differentiation factor 15 in human lens epithelial cells (2011)
  • Author(s): Yoshitake Y
  • Organizer: 36th FEBS CONGRESS
  • Place of Presentation: Lingotto Conference Center（Torino, Italy）
• [Presentation] 神経ー血管相互作用により血管で活性化される遺伝子群の同定とその機能解析 (2011)
  • Author(s): 吉冨泰央
  • Organizer: 第84回日本生化学会大会
  • Place of Presentation: 京都国際会館（京都市）
• [Presentation] Increased expression of Epac2 during in vitro tube formation in human microvascular endothelial cells (2011)
  • Author(s): 吉竹佳の
  • Organizer: 第34回日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜（横浜市）
• [Presentation] 低酸素状態はmRNA選択的3’端プロセシングを介して微小血管内皮細胞の可溶型VEGF受容体（可溶型Flt-1）産生を抑制する
  • Author(s): 池田崇之
  • Organizer: 日本生化学会北陸支部第３０回記念大会
  • Place of Presentation: 金沢歌劇座（金沢市）
  • Invited
• [Remarks]
  • URL: http://www.kanazawa-med.ac.jp/~biochem/
• [Remarks] 金沢医科大学医学部生化学II
  • URL: http://www.kanazawa-med.ac.jp/~biochem2/
• [Remarks] 金沢医科大学医学部生化学II
• [Remarks]
  • URL: http://www.kanazawa-med.ac.jp/~biochem2/