Breakthrough of the pathogenesis and treatment strategy in necrotizing enterocolitis using two-photon laser scanning microscopy based on the alarmin concept.

• Project/Area Number: 23792027
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Pediatric surgery
• Research Institution: Mie University
• Principal Investigator: KOIKE Yuhki 三重大学, 大学院・医学系研究科, 助教 (10555551)
• Project Period (FY): 2011 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000); Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2011: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: 先天性消化器疾患学 / two-photon laser-scanning microscopy (TPLSM) / necrotizing enterocolitis (NEC) model / 壊死性腸炎 / 2光子レーザー顕微鏡 / アラーミン / 敗血症

Research Abstract

This research project is making the necrotizing enterocolitis model created in beta-actin-green fluorescent protein transgenic mice observed by two-photon laser-scanning microscopy(TPLSM) , and figuring out the pathogenesis and treatment of this disease using this model and system. As for the preliminary study, we performed the LPS induced septic model mouse, and achieved the observation of dynamic pathology of intestinal microcirculation in this septic model mouse. Nest stage, we tried to performed the necrotizing enterocolitis (NEC) model in neonatal mouse induced by LPS with hypoxia (with or without hypothermia exposure).

Research Products

• [Journal Article] In vivo time-course imaging of tumor angiogenesis in colorectal liver metastases in the same living mice using two-photon laser scanning microscopy (2012)
  • Author(s): Tanaka K, Morimoto Y, Toiyama Y, Matsushita K, Kawamura M, Koike Y, Okugawa Y, Inoue Y, Uchida K, Araki T, Mizoguchi A, Kusunoki M
  • Journal Title: J Oncol Volume: 2012 Pages: 265487-265487
  • DOI: 10.1155/2012/265487
  • NAID: 120003596966
  • Peer Reviewed
• [Journal Article] In vivo real-time two-photon microscopic imaging of platelet aggregation induced by selective laser irradiation to the endothelium created in the beta-actin-green fluorescent protein transgenic mice (2011)
  • Author(s): Koike Y, Tanaka K, Okugawa Y, Morimoto Y, Toiyama Y, Uchida K, Miki C, Mizoguchi A, Kusunoki M
  • Journal Title: J Thromb Thrombolysis Volume: 32(2) Issue: 2 Pages: 138-145
  • DOI: 10.1007/s11239-011-0600-y
  • Peer Reviewed
• [Presentation] 二光子レーザー顕微鏡を用いた敗血症モデルマウスにおける Neutrophil extracellular trapsの観察と検討 (2013)
  • Author(s): 小池勇樹
  • Organizer: 第50回日本小児外科学会学術集会
  • Place of Presentation: 京王プラザホテル
• [Presentation] 2光子レーザー顕微鏡を用いた敗血症モデルマウスにおけるNeutrophil extracellular trapsの観察と検討 (2013)
  • Author(s): 小池 勇樹
  • Organizer: 第50回日本小児外科学会学術集会
  • Place of Presentation: 京王プラザホテル
• [Presentation] 二光子レーザー顕微鏡を用いたマウス敗血症モデルの消化管微小循環における病態進展メカニズムの検討 (2012)
  • Author(s): 小池勇樹
  • Organizer: 第49回日本小児外科学会学術集会
  • Place of Presentation: パシフィコ横浜
• [Presentation] 二光子レーザー顕微鏡を用いたマウス敗血症モデルにおけるReal-time intravital imagingによる腸管微小循環の観察と検討 (2012)
  • Author(s): 小池勇樹， 田中光司，井出正造， 橋本 清， 沖上正人，川村幹雄，松下航平，奥川喜永，森本雄貴， 井上幹大， 毛利靖彦，内田恵一，溝口 明，楠 正人
  • Organizer: 第112回 日本外科学会定期学術集会
  • Place of Presentation: 幕張メッセ