Mesenchymal stem cell transplantation in a nitrofen-induced congenital diaphragmatic hernia rat model
| Project/Area Number |
23792031
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Pediatric surgery
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| Research Institution | Kyushu University |
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Principal Investigator |
NAGATA Kouji 九州大学, 大学病院, 助教 (20419568)
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| Project Period (FY) |
2011 – 2013
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2012: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2011: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
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| Keywords | 再生医療 / 細胞治療 / 間葉系幹細胞 / 肺低形成 / 肺発達 / 先天性横隔膜ヘルニア / 骨髄幹細胞移植 / 肺発生 / 再生 |
|---|
| Research Abstract |
The aim of this study was to evaluate the efficacy of MSCs in a nitrofen-induced CDH rat model.Pregnant rats were exposed to nitrofen on E9.5. MSCs were isolated from the eGFP transgenic rat lungs. The MSCs were transplanted into the nitrofen-induced E12.5 rats via the uterine vein, and the E21 lung explants were harvested. The study animals were divided into three: the control group, the CDH group, and the MSC-treated CDH group. The specimens were morphologically analyzed using HE and immunohistochemical staining. The alveolar and medial walls of the pulmonary arteries were significantly thinner in the MSC-treated CDH group than in the CDH group. The alveolar air space areas were larger than in the CDH group. MSC transplantation potentially promotes alveolar and pulmonary artery development, thereby reducing the severity of pulmonary hypoplasia.
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Report
(4 results)
Research Products
(46 results)
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[Journal Article] The outcome of patients with congenital diaphragmatic hernia and having indications for a Fontan operation : Results of a national survey in Japan2014
Author(s)
Shiono N, Inamura N, Takahashi S, Nagata K, Fujino F, Hayakawa M, Usui N, Okuyama H, Kanamori Y, Taguchi T, Minakami H
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Journal Title
Pediatr Surg Int
Volume: (Accept In press)
Related Report
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[Journal Article] The current profile and outcome of congenital diaphragmatic hernia: a nationwide survey in Japan.2013
Author(s)
Nagata, K, Usui, N, Kanamori, Y, Takahashi, S, Hayakawa, M, Okuyama, H, Inamura, N, Fujino, Y, Taguchi, T
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Journal Title
J Pediatr Surg
Volume: 48
Issue: 4
Pages: 738-744
DOI
Related Report
Peer Reviewed
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[Journal Article] Prognostic Factors of Congenital Diaphragmatic Hernia Accompanied by Cardiovascular Malformation2013
Author(s)
Takahashi, S, Sago, H, Kanamori, Y, Hayakawa, M, Okuyama, H, Inamura, N, Fujino, Y, Usui, N, Taguchi, T
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Journal Title
Pediatr Int
Volume: 55
Issue: 4
Pages: 492-7
DOI
Related Report
Peer Reviewed
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