The ability of mesenchymal stem cells to initialize self-renewal of intestinal stem cells

• Project/Area Number: 23792035
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Pediatric surgery
• Research Institution: Hyogo Medical University
• Principal Investigator: NOSE Satoko 兵庫医科大学, 医学部, 助教 (90467564)
• Project Period (FY): 2011 – 2012
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000); Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2011: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
• Keywords: 組織腸上皮幹細胞 / 間葉系幹細胞移植 / 虚血再還流障害 / 再生 / 間葉系幹細胞 / 腸管上皮幹細胞 / 腸管虚血再還流障害 / 腸管上皮幹細胞Lgr5 / 骨髄間葉系幹細胞MSC

Research Abstract

The role of transplanted mesenchymal stem cells (MSCs) in the recovery after intestinal ischemia/ reperfusion injury (IRI) was investigated focusing on the intestinal stem cell function. We explored the mechanisms for the therapeutic action of engrafted MSCs that affect the epithelial cell restitution and proliferation as a result in consequences to the epithelial stem cell regulation in an IRI model. Recovery of epithelial function after IRI was significantly promoted by MSC transplantation due to inhibiting apoptosis and promoting cell proliferation. Furthermore, lacZ-labelled MSC-derived cells were identified in the area of intestinal stem cells. Engrafted MSCs were likely programmed to differentiate to intestinal stem cells.