The Possibility of Microsurgical Vascularized Nerve Flap for Spinal Cord Repair
| Project/Area Number |
23792038
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Plastic surgery
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| Research Institution | The University of Tokyo |
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Principal Investigator |
KIKUCHI Kazuki 東京大学, 医学部附属病院, 助教 (40508090)
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| Project Period (FY) |
2011 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2012: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2011: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | マイクロサージャリー / 脊髄損傷 / 血管柄付神経移植 / 中枢神経再生 / 血管付き神経移植 |
|---|
| Research Abstract |
Object: By making the “vascularized peripheral nerve flap for spinal cord injury” model, we assessed the hind limb function and the histological effect of this method.
Method: Six female Sprague-Dawley (SD) rats were divided into 2 groups, group A and B. Group A included “non-vascularized nerve flap” models. Group B consisted of “vascularized nerve flap” models. In each group, we transferred peripheral nerves (intercostal nerves) to fill the void made by surgical transection of the dorsal half of the spinal cord. Tissue sampling was done on postoperative day (POD) 90.
Results: In Group A, non-vascularized models, two out of three rats died in a few weeks and the survived one showed partial restoration of hind limb function, but it could not raise its lower back at all. In Group B, all rats survived and showed good restoration of hind limb function, they could raise their lower back absolutely. In the histological study, withneurofilament immunostaining, the density of the neurofilament in Group B was significantly higher than that in Group A. Therefore, in the vascularized cases, it was made clear that Schwann cells survived in vascularized models and that fibrotic scar formation was inhibited. This phenomenon facilitates axon guidance and results in good recovery in hind limb function.
Conclusion:Both histologically and functionally, significant differences between the vascularized and non-vascularized peripheral nerve transfer models were observed in 90 days. The fact that vascularized nerve flaps promotes axon guidance in spinal cord injury areas may result in the good functional recovery. Though a further histological study is necessary to clarify these results, but it was suggested that vascularized nerve flap become a breakthrough in spinal cord repair.
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Report
(3 results)
Research Products
(12 results)
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[Presentation]2012
Organizer
第39回日本マイクロサージャリー学会学術集会
Place of Presentation
於北九州国際会議場
Year and Date
2012-12-07
Related Report
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[Presentation]2012
Organizer
第21回日本形成外科学会基礎学術集会
Place of Presentation
於ホテルリステル猪苗代
Year and Date
2012-10-05
Related Report
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[Presentation]2012
Organizer
第55回日本形成外科学会総会・学術集会
Place of Presentation
於ホテルニューオータニ
Year and Date
2012-04-12
Related Report
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[Presentation]2011
Organizer
第38回日本マイクロサージャリー学会学術集会
Place of Presentation
於新潟コンベンションセンター
Year and Date
2011-11-11
Related Report
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[Presentation]2011
Organizer
第20回日本形成外科学会基礎学術集会
Place of Presentation
於ハイアットリージェンシー東京
Year and Date
2011-10-07
Related Report
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[Presentation]2011
Organizer
第54回日本形成外科学会総会・学術集会
Place of Presentation
於ホテルクレメント徳島
Year and Date
2011-04-14
Related Report
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