A perception reproduction mechanism in a flap and a skin graft , and a new possibility of the tacrolimus external use

• Project/Area Number: 23792060
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Plastic surgery
• Research Institution: Juntendo University
• Principal Investigator: NATORI Yuhei 順天堂大学, 医学部, 助手 (80445478)
• Project Period (FY): 2011 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2012: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2011: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
• Keywords: 皮弁 / 植皮術 / 神経再生 / トランスジェニックマウス / live imaging / FK506 / 短期的神経刺激

Research Abstract

To evaluating sensory innervation into either skin flap or skin graft, we used transgenic mice which all axons express YFP fluorescent and developed live imaing system for murine skin flap model. It was useful tool for evaluating sens ory innervation and the intensity is elevated only at the flap which sensory nerve was preserved. We also evaluated the effect of applying tacrolimus ointment on the flap, and the tacrolimus applied group showed higher intensity than the other groups. The application of the tacrolimus may promote sensory nerve regeneration into the flap.