Lineage analysis of cells involved in bone formation using the ectopic bone model in which prepared bone marrow stromal cells were transplanted in rats

• Project/Area Number: 23792066
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Plastic surgery
• Research Institution: Kurume University
• Principal Investigator: TANOUE Ryuichiro 久留米大学, 医学部, 助教 (40597457)
• Project Period (FY): 2011 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000); Fiscal Year 2012: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2011: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
• Keywords: 細胞培養 / 移植学 / 間葉系幹細胞 / 再生医学 / 形態 / 骨再生 / 骨髄間質細胞 / 幹細胞 / 線維芽細胞

Research Abstract

In our research, we employed bone marrow stromal cells that expressed Enhanced Green Flourescent Protein (EGFP) as a reporter molecule to observethe formation and organization of subcutaneous ectopic bone tissues in rats backs andto verify the origin of those cells. The results showed an even proportion of donor cellsand recipient cells in the organization of ectopic bone tissues. It was also confirmedthat some of the recipient cells were originated from fibroblasts.

Research Products

• [Journal Article] 副甲状腺ホルモンの間歇的投与がラット皮下異所性骨モデルに与える影響 (2013)
  • Author(s): 田上隆一郎、平嶋伸悟、金澤知之進、吉富宗健、太田啓介、中村桂一郎、楠川仁悟
  • Journal Title: 久留米医学会雑誌 Volume: 76 Pages: 34-41
  • NAID: 40019678618
  • Peer Reviewed
• [Journal Article] Bone marrow stromal cells cause subcutaneous fibroblasts to differentiate into osteocytes in a physically statial micronment in rats. (2013)
  • Author(s): Tanoue R
  • Journal Title: Acta Histochemca Volume: 1 Issue: 5 Pages: 1-1
  • DOI: 10.1016/j.acthis.2012.11.007
  • Peer Reviewed
• [Journal Article] 副甲状腺ホルモンの間歇的投与がラット皮下異所性骨モデルに与える影響 (2013)
  • Author(s): 田上 隆一郎
  • Journal Title: 久留米医学会雑誌 Volume: Vol.76 No.1・2
  • NAID: 40019678618
  • Peer Reviewed
• [Journal Article] The effect of the microenvironmentcreated by a titanium mesh cage onsubcutaneous experimental bone formationand inhibition of absorption. (2012)
  • Author(s): T a n o u e R, Ohta K, Kusukawa J, NakamuraK.
  • Journal Title: CellsTissues Organs Volume: 196 Issue: 3 Pages: 221-230
  • DOI: 10.1159/000334409
  • Peer Reviewed
• [Presentation] FIB/SEMトモグラフィーを用いた脱灰骨および未脱灰骨の微細構造の比較 (2012)
  • Author(s): 田上隆一郎、太田啓介、蔵田耕作、都合亜記暢、東龍平、金澤知之進、中村桂一郎
  • Organizer: 第68回日本顕微鏡学会・学術講演会
  • Place of Presentation: 筑波
  • Year and Date: 2012-05-15
• [Presentation] ラット皮下異所性新生骨のFIB/SEMトモグラフィーによる微細構造解析 (2012)
  • Author(s): 田上隆一郎、太田啓介、都合亜記暢、東龍平、楠川仁悟、中村桂一郎
  • Organizer: 第117回日本解剖学会・全国学術集会
  • Place of Presentation: 山梨
  • Year and Date: 2012-03-26
• [Presentation] Lineage analysis of cellsinvolving in bone formation usingthe subcutaneous ectopic boneformation model (2012)
  • Author(s): R. Tanoue, K. Ohta, S. Ogasawara, H. Yano, J. Kusukawa, K.Nakamura.
  • Organizer: 10th Asia-PacificMicroscopy Conference.
  • Place of Presentation: Perth, Western Australia
  • Year and Date: 2012-02-08
• [Presentation] FIB/SEMトモグラフィーを用いた脱灰骨および未脱灰骨の微細構造の比較 (2012)
  • Author(s): 田上 隆一郎
  • Organizer: 日本顕微鏡学会 第68回学術講演会
  • Place of Presentation: つくば
• [Presentation] Lineage analysis of cells involving in bone formation using the subcutaneous ectopic bone formation model (2012)
  • Author(s): Tanoue R
  • Organizer: 10th Asia-Pacific Microscopy Conference
  • Place of Presentation: Perth Australia
• [Presentation] ラット皮下異所性新生骨のFIB/SEMトモグラフィーによる微細構造解析 (2012)
  • Author(s): 田上 隆一郎
  • Organizer: 第117回日本解剖学会・全国学術集会
  • Place of Presentation: 山梨県
• [Remarks]
  • URL: http://www.med.kurume-u.ac.jp/med/anat2/
• [Remarks] 久留米大学医学部 解剖学講座 (顕微解剖・生体形成部門)
  • URL: http://www.med.kurume-u.ac.jp/med/anat2/