The assessment of oxidative stress by measuring superoxide anion radical directly in venous

• Project/Area Number: 23792074
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Emergency medicine
• Research Institution: Yamaguchi University
• Principal Investigator: TODANI Masaki 山口大学, 医学部附属病院, 助教 (00585721)
• Project Period (FY): 2011 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2011: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
• Keywords: フリーラジカル / 酸化ストレス傷害 / スーパーオキシドアニオンラジカル / 生体内持続ラジカル測定法 / アポシニン、マロン酸アルデヒド / HMGB1 / ICAM-1 / アポシニン / マロン酸アルデヒド / 前脳虚血再潅流 / 中大脳動脈閉塞

Research Abstract

This study revealed the relationship among oxidative stress, endothelial injury and early inflammation to measure superoxide anion radical directly in venous. To assess the measured current values of the electrochemical sensors, we calibrated against superoxide anion radical concentration in vitro. We examined that apocynin and allopurinol suppressed superoxide anion radical generation resulting in inhibition of oxidative stress in rat with forebrain ischemia/reperfusion.