| Project/Area Number |
23792107
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Morphological basic dentistry
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| Research Institution | Kyushu University |
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Principal Investigator |
OU Hyou 九州大学, 大学病院, 学術研究員 (20452716)
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| Project Period (FY) |
2011 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2012: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2011: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
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| Keywords | ワードTRPV4 / 口腔粘膜上皮 / 低浸透圧 / Whole-cell patch clamp / Ca2+ imaging / 頬粘膜 / ATP / TRPV4 / 機械刺激 / カルシウムイメージング / 口腔粘膜 / 電気生理 / 容積調節 |
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| Research Abstract |
I hypothesized that TRPV4 (Transient receptor potential cation channel subfamily V member 4) channels in oral mucosal epithelium have sensitivity of hypoosmolarity from saliva, and support the homeostasis of oral epithelial structure. I investigated the sensitivity of hypoosmolarity and it’s function using rats or TRPV4 knockout mice. I cultured primary buccal epithelial cells and confirmed TRPV4 expression in mRNA level and protein level. I took advantage of electrophysiological and Ca2+ imaging methods, and found TRPV4 induced currents and Ca2+ mobility using TRPV4 activators and hypotonic stimulation. With the same stimuli, there was a rise of ATP released from oral epithelial cells, which suggested ATP to be a mediator to peripheral nerves. I used the method of immunocytochemistry and found hypoosmolarity induced reorganization of actin, a kind of cytoskeleton. This suggested that TRPV4 supports intercellular contact
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