Analysis of a novel glycoprotein biosynthesis in the periodontalpathogen

• Project/Area Number: 23792110
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Morphological basic dentistry
• Research Institution: Nagasaki University
• Principal Investigator: SHOJI Mikio 長崎大学, 医歯薬学総合研究科, 助教 (10336175)
• Project Period (FY): 2011 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2012: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2011: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: 歯周病細菌 / リポ多糖

Research Abstract

We found that CTD proteins in Porphyromonas gingivalis are secreted onto the cell surface via the Por secretion system and the CTD region are processed when the proteins are bound to A-LPS. In addition, we identified the Wzz, Wzx, and WbaP proteins that are involved in the LPS biosynthesis in P. gingivalis.

Research Products

• [Journal Article] Identification of an O-antigen chain length regulator, WzzP (2013)
  • Author(s): Shoji M, Yukitake H, Sato K, ShibataY, Naito M, Aduse-Opoku J, Abiko Y, Curtis M.A., Nakayama K.
  • Journal Title: WzzP, inPorphyromonas gingivalis MicrobiologyOpen in press
• [Journal Article] Identification of Porphyromonas gingivalis proteins secreted by the Por secretion system (2013)
  • Author(s): Sato K, Yukitake H, Narita Y, Shoji M,Naito M, Nakayama K.
  • Journal Title: FEMS microbiology letters Volume: 338(1) Pages: 68-76
• [Journal Article] Identification of an O-antigen chain length regul tor, WzzP, in Porphyromonas gingivalis. (2013)
  • Author(s): Shoji M, Yukitake H, Sato K, Shibata Y, Naito M, Aduse-Opoku J, Abiko Y, Curtis MA, N kayama K.
  • Journal Title: Microbiologyopen.Epub Volume: 10 Issue: 3 Pages: 383-401
  • DOI: 10.1002/mbo3.84
  • Peer Reviewed
• [Journal Article] Identification of Porphyromonas gingivalis proteins secreted by the Por secretion system. (2013)
  • Author(s): Sato K, Yukitake H, Narita Y, Shoji M, Naito M, Nakayama K.
  • Journal Title: FEMS microbiology Letters Volume: 338 (1) Issue: 1 Pages: 68-76
  • DOI: 10.1111/1574-6968.12028
  • Peer Reviewed
• [Journal Article] Porphyromonas gingivalis における表面蛋白質の輸送と局在化に関する研究 (2012)
  • Author(s): 庄子 幹郎
  • Journal Title: 面蛋白質の輸送と局在化に関する研究 Volume: 67(3) Pages: 245-255
• [Journal Article] Porphyromonas gingivalisにおける表面蛋白質の輸送と局在化に関する研究 (2012)
  • Author(s): (庄子 幹郎)
  • Journal Title: 日本細菌学雑誌 Volume: 67 Pages: 245-255
• [Journal Article] Por secretion system-dependent secretionand glycosylation of Porphyromonas gingivalis hemin-binding protein 35 (2011)
  • Author(s): Shoji M, Sato K, Yukitake H, Kondo Y, Narita Y, Kadowaki T, Naito M, Nakayama K.
  • Journal Title: PLoS One Volume: 6(6)
• [Journal Article] Characterisation of the Porphyromonas gingivalis conjugative transposon CTnPg1: determination of the integration site and the genes essential for conjugal transfer. (2011)
  • Author(s): Naito M, Sato K, Shoji M, Yukitake H, Ogura Y, Hayashi T, Nakayama K.
  • Journal Title: Microbiology-SGM Volume: 157(7) Pages: 2022-2032
• [Journal Article] Effects of non-iron metalloporphyrins on growth and gene expression of Porphyromonas gingivalis. (2011)
  • Author(s): Yukitake H, Naito M, Sato K, Shoji M, Ohara N, Yoshimura M, Sakai E,Nakayama K.
  • Journal Title: Microbiol. Immunol. Volume: 55(3) Pages: 141-153
  • NAID: 10031121453
• [Journal Article] Por secretion system-dependent secretion and glycosylation of Porphyromonas gingivalis hemin-binding protein 35 (2011)
  • Author(s): Shoji M, Sato K, Yukitake H, Kondo Y, Narita Y, Kadowaki T, Naito M, Nakayama K
  • Journal Title: PLoS One Volume: 6 Issue: 6 Pages: 21372-21372
  • DOI: 10.1371/journal.pone.0021372
  • NAID: 120006985054
  • Peer Reviewed
• [Journal Article] Characterisation of the Porphyromonas gingivalis conjugative transposon CTnPg1 : determination of the integration site and the genes essential for conjugal transfer (2011)
  • Author(s): M.Naito, et al
  • Journal Title: Microbiology Volume: 157 Issue: 7 Pages: 2022-2032
  • DOI: 10.1099/mic.0.047803-0
  • Peer Reviewed
• [Presentation] Identification of a novel O-antigen chain length regulator, WzzP, in Porphyromonas gingivalis. (2012)
  • Author(s): Shoji M, Yukitake H, Sato K, ShibataY, Naito M, Aduse-opoku J, Abiko Y, Curtis M.A., Nakayama K.
  • Organizer: The 60thannual meeting of Japanese Association for Dental Research
• [Presentation] Identification of a component of LPS biosynthesis in Porphyromonas gingivalis. (2012)
  • Author(s): Shoji M, Yukitake H, Sato K, ShibataY, Naito M, Aduse-opoku J, Abiko Y, Curtis M.A., Nakayama K.
  • Organizer: First International Conference on Porphyromonas gingivalis and Related Bacterial Species
• [Presentation] Porphyromonas gingivalisのLPS合成に関わる新奇遺伝子の発見 (2012)
  • Author(s): 庄子幹郎, 雪竹英治, 佐藤啓子, 中根大介,内藤真理子, 柴田恭子, 安孫子宜光, 中山浩次
  • Organizer: 第85回日本細菌学会総会
• [Presentation] Identification of a novel O-antigen chain length regulator, WzzP, in Porphyromonas gingivalis. (2012)
  • Author(s): (Mikio Shoji), Hideharu Yukitake, Keiko Sato, Yasuko Shibata, Mariko Naito, Joseph Aduse-opoku, Yoshimitsu Abiko, Michael A. Curtis, Koji Nakayama
  • Organizer: The 60th annual meeting of Japanese Association for Dental Research
  • Place of Presentation: 新潟市
• [Presentation] Identification of a component of LPS biosynthesis in Porphyromonas gingivalis. (2012)
  • Author(s): (Mikio Shoji), Hideharu Yukitake, Keiko Sato, Yasuko Shibata, Mariko Naito, Joseph Aduse-opoku, Yoshimitsu Abiko, Michael A. Curtis, Koji Nakayama
  • Organizer: First International Conference on Porphyromonas gingivalis and Related Bacterial Species
  • Place of Presentation: 長崎市
• [Presentation] Porphyromonas gingivalisのLPS合成に関わる新奇遺伝子の発見 (2012)
  • Author(s): 庄子幹郎、雪竹英治、佐藤啓子、中根大介、内藤真理子、柴田恭子、安孫子宜光、中山浩次
  • Organizer: 第85回日本細菌学会総会
  • Place of Presentation: 長崎市 長崎ブリックホール
• [Presentation] Porphyromonas gingivalis HBP35蛋白の菌体表面局在化機構の解析 (2011)
  • Author(s): 庄子幹郎, 佐藤啓子, 雪竹英治, 近藤好夫, 成田由香,門脇知子,内藤真理子, 安孫子宜光, 中山浩次
  • Organizer: 第53回歯科基礎医学会学術大会・総会
• [Presentation] Por secretion system-dependent secretion and glycosylation of Porphyromonas gingivalisHemin-binding protein 35 (HBP35) (2011)
  • Author(s): Shoji M, Sato K, Yukitake H, Kondo Y, Narita Y, Kadowaki T, Naito M, Nakayama K.
  • Organizer: XIII International Congress of Bacteriology and Applied Microbiology
• [Presentation] Porphyromonas gingivalis Hemin-Binding Protein 35 (HBP35) is secreted by the Por Secretion System. (2011)
  • Author(s): Shoji M, Sato K, Yukitake H, Kondo Y, Narita Y, Kadowaki T, Naito M, Nakayama K.
  • Organizer: The Gordon Research Conference on Microbial Adhesion & Signal Transduction
• [Presentation] Porphyromonas gingivalis HBP35蛋白は、Por Secretion System依存性に菌体表面に局在化する。 (2011)
  • Author(s): 庄子幹郎, 佐藤啓子, 雪竹英治, 近藤好夫, 成田由香,門脇知子,内藤真理子, 安孫子宜光, 中山浩次
  • Organizer: 第25回Bacterial Adherence and Biofilm 学術集会
• [Presentation] Porphyromonas gingivalis HBP35蛋白は、Por Secretion System依存性に菌体表面に局在化する。 (2011)
  • Author(s): 庄子幹郎、佐藤啓子、雪竹英治、近藤好夫、成田由香、門脇知子、内藤真理子、中山浩次
  • Organizer: 第25回Bacterial Adherence and Biofilm 学術集会
  • Place of Presentation: 東京都 東京慈恵医科大学
• [Presentation] Porphyromonas gingivalis Hemin-Binding Protein 35 (HBP35) is secreted by the Por Secretion System. (2011)
  • Author(s): 庄子幹郎、佐藤啓子、雪竹英治、近藤好夫、成田由香、門脇知子、内藤真理子、中山浩次
  • Organizer: The Gordon Research Conference on Microbial Adhesion & Signal Transduction
  • Place of Presentation: アメリカ ニューポート
• [Presentation] Por secretion system-dependent secretion and glycosylation of Porphyromonas gingivalis Hemin-binding protein 35. (2011)
  • Author(s): 庄子幹郎、佐藤啓子、雪竹英治、近藤好夫、成田由香、門脇知子、内藤真理子、中山浩次
  • Organizer: IUMS2011
  • Place of Presentation: 札幌市 札幌コンベンションセンター
• [Presentation] Porphyromonas gingivalis HBP35蛋白は、Por Secretion System依存性に菌体表面に局在化する。 (2011)
  • Author(s): 庄子幹郎、佐藤啓子、雪竹英治、近藤好夫、成田由香、門脇知子、内藤真理子、中山浩次
  • Organizer: 第53回歯科基礎医学会学術大会・総会
  • Place of Presentation: 岐阜市 長良川国際会議場
• [Remarks] 長崎大学歯学部 歯周病基盤研究センター
  • URL: http://www.de.nagasaki-u.ac.jp/perio/
• [Remarks] 長崎大学歯学部 歯周病基盤研究センター
  • URL: http://www.de.nagasaki-u.ac.jp/perio/
• [Remarks]
  • URL: http://www.de.nagasaki-u.ac.jp/perio/
• [Remarks] 長崎大学歯周病基盤研究センター
  • URL: http://www.nagasaki-u.ac.jp/ja/about/info/news/news926.html
• [Remarks] 日本細菌学会「黒屋奨学賞」受賞