Genome sequencing and mutational analysis of the Actinomyces strains with enhanced biofilm phenotype

• Project/Area Number: 23792118
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Morphological basic dentistry
• Research Institution: Osaka Dental University
• Principal Investigator: NAMBU Takayuki 大阪歯科大学, 歯学部, 講師 (80367903)
• Project Period (FY): 2011 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000); Fiscal Year 2012: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2011: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
• Keywords: 口腔細菌学 / バイオフィルム / 口腔細菌

Research Abstract

To find out the mechanism of biofilm formation of oral Actinomyces, genome and genetic analysis were carried out for two strains of K20 and MG-1 keeping enhanced biofilm phenotype. The genome of the strain K20 was almost completely sequenced and the given data was uploaded to the public database（DDBJ, BABV01000001-BABV01000771）. Additionally, I have identified some biofilm-related genes by mutation analysis and have reported this.

Research Products

• [Journal Article] Identification of disulphide stress-responsive extracytoplasmic function sigma factors in Rothia mucilaginosa (2013)
  • Author(s): Nambu T, Yamane K, Yamanaka T, Mashimo C, Maruyama H, Yoshida M, Hayashi H, Fukushima H
  • Journal Title: Archives of Oral Biology Volume: 58(6) Issue: 6 Pages: 681-689
  • DOI: 10.1016/j.archoralbio.2012.10.017
  • Peer Reviewed
• [Journal Article] Biofilm-forming capacity on clinically isolated Streptococcus constellatus from an odontogenic subperiosteal abscess lesion (2013)
  • Author(s): Yamanaka T, Furukawa T, Yamane K, Nambu T, Mashimo C, Maruyama H, Inoue J, Kamei M, Yasuoka H, Horiike S, Leung KP, Fukushima H
  • Journal Title: Journal of Bacteriology & Parasitology Volume: 4 Issue: 01 Pages: 1000160-1000160
  • DOI: 10.4172/2155-9597.1000160
  • Peer Reviewed
• [Journal Article] Identification of the genes involved in the biofilm-like structures on Actinomyces oris K20, a clinical isolate from an apical lesion (2013)
  • Author(s): Mashimo C, Kamitani M, Nambu T, Yamane K, Yamanaka T, Tatami T, Inoue J, Kamei M, Morita S, Fukushima H
  • Journal Title: Jounal of Endodontics Volume: 39(1) Issue: 1 Pages: 44-48
  • DOI: 10.1016/j.joen.2012.08.009
  • Peer Reviewed
• [Journal Article] Pathogenicity of exopolysaccharide-producing Actinomyces oris isolated from an apical abscess lesion (2013)
  • Author(s): Yamane K, Nambu T, Yamanaka T, Ishihara K, Tatami T, Mashimo C, Walker CB, Leung K.-P., Fukushima H
  • Journal Title: International Endodontic Journal Volume: 46(2) Issue: 2 Pages: 145-154
  • DOI: 10.1111/j.1365-2591.2012.02099.x
  • Peer Reviewed
• [Journal Article] Isolation of abiofilm-defective mutant ofActinomycesfrom a patient with gingivitis (2012)
  • Author(s): Ogiso K, Nambu T, Kotsu Y, FukushimaH, Ueda M
  • Journal Title: Journal ofOsaka Dental University Volume: 46 Pages: 91-99
  • URL: http://ci.nii.ac.jp/naid/110009436540
  • Peer Reviewed
• [Journal Article] Analysis of genes involvein exopolysaccharide production of Rothiamucilaginosaclinically isolated from apersistent apical periodontitis lesion (2012)
  • Author(s): Fujihira T, Yamane K, Yoshida M, NambuT, Hayashi H.
  • Journal Title: Journal of Osaka Dental University Volume: 46 Pages: 157-164
  • URL: http://ci.nii.ac.jp/naid/110009436547
  • Peer Reviewed
• [Journal Article] Comparison of the virulence ofexopolysaccharide-producingPrevotellaintermedi to exoplysaccharidenon-producing periodontopathic organisms (2011)
  • Author(s): Yamanaka T, Yamane K, Furukawa T, ashimo C, Sugimori C, Nambu T, Obata N,Walker CB, Leung K-P, Fukushima H
  • Journal Title: BMC Infectious Diseases Volume: 11 Issue: 1 Pages: 228-228
  • DOI: 10.1186/1471-2334-11-228
  • Peer Reviewed
• [Presentation] Genetic analysis ofbiofilm-formingStreptococcusconstellatus (2013)
  • Author(s): Yamane K, Yamanaka T, Mashimo C, NambuT, Maruyama H, Walker CB, Leung K-P,Fukushima H
  • Organizer: IADR/AADR/CADR GeneralSession & Exhibition
  • Place of Presentation: Seattle, Washington, USA
  • Year and Date: 2013-03-21
• [Presentation] 口腔アクチノマイセスにおけるバイオフィルム欠損株の同定と特徴づけ (2013)
  • Author(s): 南部隆之,真下千穂,山根一芳,山中武志,福島久典
  • Organizer: 日本細菌学会
  • Place of Presentation: 千葉市
  • Year and Date: 2013-03-18
• [Presentation] pJRD215を基盤にしたクローニング・発現用プラスミドの作成とActinomyces属細菌への応用 (2013)
  • Author(s): 真下千穂,南部隆之,山根一芳,山中武志,福島久典
  • Organizer: 日本細菌学会
  • Place of Presentation: 千葉市
  • Year and Date: 2013-03-18
• [Presentation] アユ冷水病菌の完全ゲノム配列決定とニジマス冷水病菌との比較ゲノム解析 (2013)
  • Author(s): 大黒健二,田村真奈美,南部隆之,福島久典,阿部貴志,向由紀夫
  • Organizer: ゲノム微生物学会
  • Place of Presentation: 滋賀県長浜市
  • Year and Date: 2013-03-08
• [Presentation] Characterization of Tn5 insertion mutantsof oralActinomyces affected in biofilmformation (2012)
  • Author(s): Nambu T, Mashimo C, Yamane K, YamanakaT, Maruyama H, Fukushima H
  • Organizer: Biofilms 5
  • Place of Presentation: Paris, France
  • Year and Date: 2012-12-12
• [Presentation] Sucrose independent exopolysaccharideproductivity and biofilm formation of oralcommensal bacteria as pathogenesis ofchronic inflammation in the oral cavity (2012)
  • Author(s): Yamanaka T, Yamane K, Mashimo C, NambuT, Maruyama H, Leung KP, Fukushima H
  • Organizer: Clinical microbiology and microbialgenomics
  • Place of Presentation: San Antonio,USA
  • Year and Date: 2012-11-12
• [Presentation] 口腔アーキアによって疾患は起こるか (2012)
  • Author(s): 円山由郷,山根一芳,南部隆之,真下千穂,山中武志,福島久典
  • Organizer: 日本歯科医学会総会
  • Place of Presentation: 大阪市
  • Year and Date: 2012-11-10
• [Presentation] 口腔感染症の病因論が変わった (2012)
  • Author(s): 山根一芳,円山由郷,南部隆之,真下千穂,山中武志,福島久典
  • Organizer: 日本歯科医学会総会
  • Place of Presentation: 大阪市
  • Year and Date: 2012-11-10
• [Presentation] 矯正線表面へのActinomycesorisMG‐1株の付着性 (2012)
  • Author(s): 大前有紀,山根一芳,南部隆之,松本尚之
  • Organizer: 日本矯正歯科学会大会
  • Place of Presentation: 郡山市
  • Year and Date: 2012-09-28
• [Presentation] Development of new plasmids for cloning and expression in oral Actinomyces spp (2012)
  • Author(s): Mashimo C, Nambu T, Maruyama H, Yamane K, Yamanaka T, Fukushima H
  • Organizer: The 4th EMBO meeting2012
  • Place of Presentation: Nice, France
  • Year and Date: 2012-09-24
• [Presentation] 広宿主域接合プラスミドpJRD215を基盤にした新規プラスミドの作製と口腔Actinomyces属細菌への応用 (2012)
  • Author(s): 真下千穂,南部隆之,円山由郷,山根一芳,山中武志,福島久典
  • Organizer: 第26回BacterialAdherence&Biofilm学術集会
  • Place of Presentation: 大阪市
  • Year and Date: 2012-07-13
• [Presentation] Characterization of the diamide stress stimulon in Rothia mucilaginosa (2012)
  • Author(s): Nambu T, Yamane K, Yamanaka T, Mashimo C, K-P Leung, Fukushima H
  • Organizer: Eurobiofilms 2011
  • Place of Presentation: Copenhagen, Denmark
  • Year and Date: 2012-07-07
• [Presentation] Genetic analysis of exopolysaccharide-producing Rothia mucilaginosa isolated from a persistent apical periodontitis legion (2012)
  • Author(s): Fujihira T, Yamane K, Nambu T, Yoshida M, Mashimo C, Yamanaka T, K-P Leung, Fukushima H, Hayashi H
  • Organizer: Eurobiofilms2011
  • Place of Presentation: Copenhagen, Denmark
  • Year and Date: 2012-07-07
• [Presentation] Construction of mutants with reduced biofilm formation from Actinomyces oris MG-1 by transposon mutagenesis (2012)
  • Author(s): Ogiso K, Nambu T, Yamanaka T, Yamane K, Mashimo C, Kotsu Y, K-P Leung, Fukushima H, Ueda M
  • Organizer: Eurobiofilms 2011
  • Place of Presentation: Copenhagen, Denmark
  • Year and Date: 2012-07-06
• [Presentation] バイオフィルム形成Streptococcusintermediusのゲノム解析 (2012)
  • Author(s): 山根一芳,山中武志,真下千穂,吉田匡宏,林宏行,南部隆之,円山由郷,福島久典
  • Organizer: 日本歯科保存学会平成24年度春季大会
  • Place of Presentation: 宜野湾市
  • Year and Date: 2012-06-29
• [Presentation] Characterization of Tn5 insertion mutants of oral Actinomyces affected in biofilm formation (2012)
  • Author(s): Nambu T, Mashimo C, Yamane K, Yamanaka T, Maruyama H, Fukushima H
  • Organizer: Biofilms 5
  • Place of Presentation: Paris, France
• [Presentation] Actinomycesorisにおけるバイオフィルム関連遺伝子の同定 (2011)
  • Author(s): 小木曽一貴,南部隆之,高津兆雄,福島久典,上田雅俊
  • Organizer: 日本歯周病学会
  • Place of Presentation: 下関市
  • Year and Date: 2011-09-24
• [Presentation] Gene expression profiles of Rothia mucilaginosa grown under diamide stress (2011)
  • Author(s): Nambu T, Yamane K, Yamanaka T, Mashimo C, Fukushima H
  • Organizer: International Union of Microbiological Societies 2011 Congress
  • Place of Presentation: 札幌市
  • Year and Date: 2011-09-10
• [Presentation] Production of polyclonal antibodies raised against Prevotella intermedia GroEL and DnaK proteins (2011)
  • Author(s): Yamanaka T, Sugimori C, Yamane K, Nambu T, Mashimo C, Fukushima H
  • Organizer: International Union of Microbiological Societies 2011Congress
  • Place of Presentation: 札幌市
  • Year and Date: 2011-09-10
• [Presentation] Pathogenicity of exopolysaccharide-producing Actinomyces oris isolated from an apical abscess lesion (2011)
  • Author(s): Yamane K, Yamanaka T, Nambu T, MashimoC, Fukushima H
  • Organizer: International Union of Microbiological Societies 2011 Congress
  • Place of Presentation: 札幌市
  • Year and Date: 2011-09-10
• [Presentation] Genetic analysis of a Gram negative wide host-range plasmid pJRD215 and its application to oral Actinomyces spp (2011)
  • Author(s): Mashimo C, Nambu T, Yamane K, Yamanaka T, Fukushima H
  • Organizer: International Union of Microbiological Societies 2011 Congress
  • Place of Presentation: 札幌市
  • Year and Date: 2011-09-10
• [Presentation] 歯肉炎病巣より分離されたActinomycesからの付着能欠損株の単離 (2011)
  • Author(s): 小木曽一貴,南部隆之,高津兆雄,福島久典,上田雅俊
  • Organizer: 日本歯周病学会
  • Year and Date: 2011-05-28
• [Presentation] Actinomyces orisにおけるバイオフィルム関連遺伝子の同定 (2011)
  • Author(s): 小木曽一貴, 南部隆之, 高津兆雄, 福島久典, 上田雅俊
  • Organizer: 日本歯周病学会
  • Place of Presentation: 下関市
• [Presentation] Genetic analysis of biofilm-forming Streptococcus constellatus
  • Author(s): Yamane K, Yamanaka T, Mashimo C, Nambu T, Maruyama H, Walker CB, Leung K-P, Fukushima H
  • Organizer: IADR/AADR/CADR General Session & Exhibition
  • Place of Presentation: Seattle, Washington, USA
• [Presentation] Sucrose independent exopolysaccharide productivity and biofilm formation of oral commensal bacteria as pathogenesis of chronic inflammation in the oral cavity
  • Author(s): Yamanaka T, Yamane K, Mashimo C, Nambu T, Maruyama H, Leung KP, Fukushima H
  • Organizer: Clinical microbiology and microbial genomics
  • Place of Presentation: San Antonio, USA
• [Presentation] Development of new plasmids for cloning and expression in oral Actinomyces spp.
  • Author(s): Mashimo C, Nambu T, Maruyama H, Yamane K, Yamanaka T, Fukushima H
  • Organizer: The 4th EMBO meeting 2012
  • Place of Presentation: Nice, France