| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2013: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2012: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2011: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Research Abstract |
Na+/K+ ATPase is one of the marker protein which is transported to the basolateral membrane in salivary gland. We made the plasmid vector which express Na+/K+ ATPase fused with Halo tag and transfected it to the HeLa cells.
We analyzed the transport dynamics of newly synthesized Halo tag fusing with Na+/K+ ATPase by using blocking ligand and fluorescence ligand, and we have shown that most of Na+/K+ ATPase are not transported via endosome. We made adeno virus which express Halo-tag Na+/K+ ATPase and transfected it to the submandibular gland, and we confirmed that molecules are expressed on the basolateral membrane of submandibular gland by immunostaining. Transfected submandibular gland was dispersed by digestive enzyme and stained with fluorescent ligands, and we could observe the specific staining of plasma membrane in living cells.
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