| Project/Area Number |
23792130
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Functional basic dentistry
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| Research Institution | Iwate Medical University |
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Principal Investigator |
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| Project Period (FY) |
2011 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2011: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
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| Keywords | 口腔生化学 / 硫化水素産生 / 歯周病 / 酵素 / 立体構造 / 硫化水素 / Fusobacterium |
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| Research Abstract |
Hydrogen sulfide produced by oral bacteria is a predominant causative agent of oral malodor and is also known to be a pathogenic factor of periodontal disease. In this research, we determined the structures of two hydrogen sulfide-producing enzymes in Fusobacterium nucleatum, a periodontal pathogen that can produce a significant amount of hydrogen sulfide in human oral cavity. Furthermore, we analyzed enzymatic properties of the wild types and site -directed mutants. Based on the results , the reaction mechanisms of the enzymes were elucidated, and molecular and structural basis of hydrogen sulfide-producing mechanism in this oral bacterium could be established.
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