Roles of a heretical Wnt receptor, Ryk for bone remodeling

• Project/Area Number: 23792135
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Functional basic dentistry
• Research Institution: Matsumoto Dental University
• Principal Investigator: NAKAMICHI Yuko 松本歯科大学, 総合歯科医学研究所, 講師 (20350829)
• Project Period (FY): 2011 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2011: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: 骨代謝 / Wnt, Ryk, 骨芽細胞 / 破骨細胞 / 発生・分化 / 破骨細胞前駆細胞 / Ryk / Wnt

Research Abstract

The purpose of this study is clarifying a role of atypical Wnt receptor Ryk in the bone metabolism. Ryk was called a heretical Wnt receptor because of its function and properties in both canonical and non-canonical Wnt pathway. Ryk binds to Wnt3a (a canonical ligand) and Wnt5a (a non-canonical ligand), and transduces signals to control many biological processes. Ryk is expressed in both osteoclast-lineage cells and osteoblasts. Wegenerated conditional Ryk knockout mice to delete Ryk gene in these cells.

Research Products

• [Journal Article] Spleen serves as a reservoir of osteoclast precursors through vitamin D-induced IL-34 expression in CSF-1^<op/op>mice (2012)
  • Author(s): Nakamichi Y et al.
  • Journal Title: PNAS. Jun Volume: 109 Pages: 10006-1
  • DOI: 10.1073/pnas.1207361109
  • Peer Reviewed
• [Journal Article] c-Fos plays an essential role in the up-regulation of RANK expression in osteoclast precursors within the bone microenvironment (2012)
  • Author(s): Arai A et al.
  • Journal Title: J Cell Sci. Feb; Volume: 125 Pages: 2910-17
  • DOI: 10.1242/jcs.099986
  • Peer Reviewed
• [Journal Article] Daily administration of eldecalcitol (ED-71), an active vitamin D analog, increases bone mineral density by suppressing RANKL expression in mouse trabecular bone. (2012)
  • Author(s): Harada S et al.
  • Journal Title: J. Bone Miner. Res. Volume: 27 Pages: 461-473
  • DOI: 10.1002/jbmr.555
  • Peer Reviewed
• [Journal Article] Lineage-committed osteoclast precursors circulate in blood and settle down into bone. (2012)
  • Author(s): Muto, A, Mizoguchi, T, Udagawa, N., Ito, S, Kawahara, I, Abiko, Y, Arai, A., Harada, S., Kobayashi, Y., Nakamichi, Y, Penninger, JM, Noguchi, T, Takahashi, N.
  • Journal Title: J Bone MinerRes.Dec; Volume: 26 Pages: 2978-2990
  • DOI: 10.1002/jbmr.490
  • Peer Reviewed
• [Journal Article] 破骨細胞前駆細胞の形成と供 給におけるビタミン D の役割の新知見 (2011)
  • Author(s): 中道裕子
  • Journal Title: THE BONE Volume: 25 Pages: 233-237
  • URL: http://www.de-hon.ne.jp/digital/tmp/4A03DDAE2E9E40899044B70D623658FC/2000003264009800600P0B.pdf
• [Journal Article] Rab27a and Rab27b are involved in stimulation-dependent RANKL release from secretory lysosomes in osteoblastic cells (2011)
  • Author(s): Kariya Y, et al
  • Journal Title: J Bone Miner Res Volume: 26 Pages: 689-703
  • DOI: 10.1002/jbmr.268
  • Peer Reviewed
• [Journal Article] Hypergravity suppresses bone resorption in ovariectomized rat. (2011)
  • Author(s): Ikawa T, et al
  • Journal Title: Adv Space Res Volume: 47 Pages: 1214-1224
  • DOI: 10.1016/j.asr.2010.12.004
  • Peer Reviewed
• [Presentation] 破骨細胞形成におけるM-CSF/IL-34/RANKL のそれぞれの役割」 (2012)
  • Author(s): 中道裕子 ;
  • Organizer: 第15回骨発生・再生研究会
  • Place of Presentation: 慶応大学(東京都新宿区)
  • Year and Date: 2012-11-10
• [Presentation] M-CSF 欠損 op/op マウスに おいて,脾臓は破骨細胞前駆細胞の貯蔵 庫として機能する:破骨細胞形成におけるIL-34 の新しい役割 (2012)
  • Author(s): 中道裕子 ,溝口利英,荒井敦,小林泰浩, 保田尚孝,加藤茂明,須田立雄,宇田川信 之,高橋直之
  • Organizer: 日本骨代謝学会学術 集会 (第30回)
  • Place of Presentation: 京王プ ラザホテル(東京都新宿区)
  • Year and Date: 2012-07-21
• [Presentation] Spleen serves as a reservoir of osteoclast precursors through IL-34 expression in op/op mice (2012)
  • Author(s): Nakamichi Y, Mizoguchi T, Arai A, Kobayashi Y, Penninger JM, Yasuda H, Kato S, DeLuca HF, Suda T, Udagawa N, Takahashi N
  • Organizer: 4th InternationalConference on Osteoimmunology(第4回国際骨免疫学会議)
  • Place of Presentation: Chandris Hotel(コルフ島,ギリシア)
  • Year and Date: 2012-06-21
• [Presentation] 破骨細胞形成におけるM-CSF/RANKL/IL-34のそれぞれの役割 (2012)
  • Author(s): 中道裕子,荒井敦,小林泰浩,宇田川信之,高橋直之;
  • Organizer: 松本歯科大学学会例会(第75回)
  • Place of Presentation: 松本歯科大学(長野県塩尻市
  • Year and Date: 2012-01-21
• [Presentation] 歯髄細胞の有する顕著な骨再生能力は、annexin A8を介して発揮される (2011)
  • Author(s): 中道裕子，萩原貴寛，中村美どり，高橋直之，宇田川信之
  • Organizer: 日本骨代謝学会 (第29回)
  • Place of Presentation: 大阪国際会議場（大阪市）
• [Presentation] Dental pulp cells exhibit high performance as a biomaterial for bone formation through expression of annexin A8. (2011)
  • Author(s): Nakamichi Y, Hagihara T, Nakamura M, Imaoka A, Abiko Y, Nakamura H, Takahashi N, Udagawa N
  • Organizer: 33th American Society for Bone and Mineral Research (ASBMR)
  • Place of Presentation: サンディエゴコンベンションセンター (USA)
• [Presentation] Dental Pulp Cells Exhibit High Performance as a Biomaterial for Bone Formation Through Expression of Annexin A8 (2011)
  • Author(s): Nakamichi Y, Hagihara T, Nakamura M, Imaoka A, Abiko Y, Nakamura H, Takahashi N, Udagawa N.
  • Organizer: 第31回米国骨代謝学会
  • Place of Presentation: サンディエゴコンベンションセンター(カリフォルニア州,米国)
  • Year and Date: 2011-09-17
• [Presentation] 歯髄細胞の有する顕著な骨再生能力は、annexin A8を介して発揮される (2011)
  • Author(s): 中道裕子,萩原貴寛,中村美どり,高橋直之,宇田川信之
  • Organizer: 日本骨代謝学会 (第29回)
  • Place of Presentation: 大阪国際会議場(大阪市北区)
  • Year and Date: 2011-07-30
• [Presentation] Spleen serves as a reservoir of osteoclast precursors through IL-34 expression in op/op mice: A pivotal role of IL-34 in osteoclastogenesis
  • Author(s): 中道裕子
  • Organizer: 第4回国際骨免疫学会議
  • Place of Presentation: ギリシア、コルフ島
• [Presentation] 破骨細胞形成におけるM-CSF/IL-34/RANKLのそれぞれの役割
  • Author(s): 中道 裕子
  • Organizer: 第15回骨発生・再生研究会
  • Place of Presentation: 東京 (慶応大学)
  • Invited