Effects of anti-angiogenesis by HDAC inhibitor and DNA methylation inhibitor
Project/Area Number |
23792159
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Research Category |
Grant-in-Aid for Young Scientists (B)
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Allocation Type | Multi-year Fund |
Research Field |
Pathobiological dentistry/Dental radiology
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Research Institution | Hyogo Medical University |
Principal Investigator |
YAMANEGI Koji 兵庫医科大学, 医学部, 講師 (00434944)
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Project Period (FY) |
2011 – 2012
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Project Status |
Completed (Fiscal Year 2012)
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Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2012: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2011: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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Keywords | 骨肉腫、 / HDAC inhibitor、 / DNA methylation inhibitor、 / 腫瘍血管新生 / VEGI / HDAC inhibitor / 骨肉腫 / ヒストン脱アセチル化阻害剤 / VEGI (TL1A) |
Research Abstract |
We investigated the effects of anti-angiogenesis by valproic acid (VPA) (HDAC inhibitor) in combination with hydralazine (Hy) (DNA methylation inhibitor) on U-2 OS and SaOS-2 human osteosarcoma cells. Vascular endothelial growth inhibitor (VEGI) are potent inhibitors to suppress endothelial cell proliferation, angiogenesis, also tumor growth and neovascularization. These inhibitions are mediated by death receptor 3 (DR3) which induces apoptosis, and its function is blocked by decoy receptor 3 (DcR3). VPA increased VEGI expression and Hy increased DR3 expression. Their combination induced further increasing effect of both VEGI and DR3 without induction of DcR3 on osteosarcomas and human microvascular endothelial cells. Furthermore, VPA treated medium of osteosarcoma cells can inhibit tube formation in vitro. Thus, these results, together, suggest that VPA and Hy are considered to be one of the promising strategies in the development of novel anticancer therapy.
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Report
(3 results)
Research Products
(26 results)
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[Journal Article] Decreased expression of VE-cadherin and claudin-5 and increased phosphorylation of VE-cadherin in vascular endothelium in nasal polyps.2013
Author(s)
Yukitatsu Y, Hata M, Yamanegi K, Yamada N, Ohyama H, Nakasho K, Kojima Y, Oka H, Tsuzuki K, Sakagami M, Terada N.
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Journal Title
Cell Tissue Res
Volume: 352
Issue: 3
Pages: 647-657
DOI
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Peer Reviewed
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[Journal Article] Downregulation of matrix metalloproteinase-9 mRNA by valproic acid plays a role in inhibiting the shedding of MHC class I-related molecules A and B on the surface of human osteosarcoma cells.2012
Author(s)
Yamanegi K, Yamane J, Kobayashi K, Ohyama H, Nakasho K, Yamada N, Hata M, Fukunaga S, Futani H, Okamura H, Terada N.
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Journal Title
Oncol Rep.
Volume: 28(5)
Issue: 5
Pages: 1585-1590
DOI
Related Report
Peer Reviewed
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[Journal Article] Valproic acid cooperates with hydralazine to augment the susceptibility of human osteosarcoma2012
Author(s)
Yamanegi K, Yamane J, Kobayashi K, Kato-Kogoe N, Ohyama H, Nakasho K, YamadaN, Hata M, Fukunaga S, Futani H, Okamura H, Terada N.
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Journal Title
cells to Fas- and NK cell-mediated cell death.Int J Oncol.
Volume: 41(1)
Pages: 83-91
DOI
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Peer Reviewed
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[Journal Article] The promotional effect of IL-22 on mineralization activity of periodontal ligament cells2012
Author(s)
Kato-Kogoe N, Nishioka T, Kawabe M, Kataoka F, Yamanegi K, Yamada N, Hata M, Yamamoto T, Nakasho K, Urade M, Terada N, Ohyama H
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Journal Title
Cytokine
Volume: 59(1)
Issue: 1
Pages: 41-8
DOI
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Peer Reviewed
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[Journal Article] Valproic acid cooperates with hydralazine to augment the susceptibility of human osteosarcoma cells to Fas- and NK cell-mediated cell death2012
Author(s)
Koji Yamanegi, Junko Yamane, Kenta Kobayashi, Nahoko Kato-Kogoe, Hideki Ohyama, Keiji Nakasho, Naoko Yamada, Masaki Hata, Satoru Fukunaga, Hiroyuki Futani, Haruki Okamura and Nobuyuki Terada
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Journal Title
International Journal of Oncology
Volume: In Press
Related Report
Peer Reviewed
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[Presentation] Comprehensive human cell database toward cell differentiation analysis2012
Author(s)
Hatano, A., Chiba, H., Taniguchi, T., Yamanegi, K., Takai, T., Tanaka, H., Fujibuchi, W., CELLPEDIA
Organizer
Systems Biology : Global regulation of gene expression
Place of Presentation
New York, USA
Year and Date
2012-03-22
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