Gene Expression Analysis of Non-collagenous Proteins in GaAlAs Laser Irradiated Rat Molars

• Project/Area Number: 23792167
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Conservative dentistry
• Research Institution: Niigata University
• Principal Investigator: SHIGETANI Yoshimi 新潟大学, 医歯学系, 助教 (80397132)
• Project Period (FY): 2011 – 2013
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2013: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2012: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2011: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
• Keywords: 象牙質・歯髄複合体 / レーザー / 免疫組織化学的 / 遺伝子 / 免疫組織化学

Research Abstract

Irradiation of gallium-aluminum-arsenide (GaAlAs) laser to rat molars induces mineralized tissue formation in the pulp chamber. This study aimed to analyze mRNA expression of mineralized tissue matrix proteins during early phases of the laser-induced mineralized tissue formation.
Expression levels of mineralized tissue matrix protein mRNAs peaked prior to the mineralized tissue formation after laser irradiation. These proteins might play some role in the cytodifferentiation of newly-generated mineralized tissue-forming cells.

Research Products

• [Journal Article] M2 macrophages participate in the biological tissue healing reaction to mineral trioxide aggregate (2014)
  • Author(s): Ito T, Kaneko T, Yamanaka Y, Shigetani Y, Yoshiba K, Okiji T
  • Journal Title: Journal of Endodontics Volume: 40 Issue: 3 Pages: 379-383
  • DOI: 10.1016/j.joen.2013.11.011
  • Peer Reviewed
• [Journal Article] Odontoblast response to cavity preparation with Er : YAG laser in rat molars: an immunohistochemical study (2013)
  • Author(s): Shigetani Y, Suzuki H, Ohshima H, Yoshiba K, Yoshiba N, Okiji T
  • Journal Title: Odontology Volume: 101(2) Pages: 186-192
  • NAID: 10031194888
  • Peer Reviewed
• [Journal Article] Evaluation of the responses of MHC class II molecule-expressing cells and macrophages to epoxy resin-based and 4-META-containing, methacrylate resinbased root canal sealers in rat subcutaneous tissue (2013)
  • Author(s): Yamanaka Y, Shigetani Y, Yoshiba K, Kaneko T, Yoshiba N, Okiji T
  • Journal Title: Dent Mater J Volume: 32(5)
  • Peer Reviewed
• [Journal Article] Mineral trioxide aggregateに対するラット皮下結合組織の応答-マクロファージ関連分子の免疫組織化学的・分子生物学的解析- (2013)
  • Author(s): 伊藤崇史,山中裕介,金子友厚,吉羽邦彦, 吉羽永子, 重谷佳見, 興地隆史
  • Journal Title: 日歯保存誌 Volume: 56(1) Pages: 9-16
  • NAID: 110009596776
  • Peer Reviewed
• [Journal Article] ヒト歯髄創傷治癒過程における細胞外基質の局在変化−Fibrillin-1基質の動的リモデリングに関する検索− (2013)
  • Author(s): 吉羽永子,吉羽邦彦,大倉直人,重谷佳見,武井絵梨花,細矢明宏,中村浩彰,興地隆史
  • Journal Title: 日歯保存誌 Volume: 56(3) Pages: 161-168
  • NAID: 110009674425
  • Peer Reviewed
• [Journal Article] Evaluation of the responses of MHC class II molecule-expressing cells and macrophages to epoxy resin-based and 4-META-containing, methacrylate resin-based root canal sealers in rat subcutaneous tissue (2013)
  • Author(s): Yamanaka Y, Shigetani Y, Yoshiba K, Kaneko T, Yoshiba N, Okiji T
  • Journal Title: Dental Materials Journal Volume: 32 Issue: 5 Pages: 822-827
  • DOI: 10.4012/dmj.2013-005
  • NAID: 130004519306
  • ISSN: 0287-4547, 1881-1361
  • Peer Reviewed
• [Journal Article] Expressional alterations of fibrillin-1 during wound healing of human dental pulp (2012)
  • Author(s): Yoshiba N, Yoshiba K, Ohkura N, Hosoya A, Shigetani Y, Yamanaka Y, Izumi N, Nakamura H, Okiji T
  • Journal Title: Journal of Endodontics Volume: 38(2) Pages: 177-184
  • Peer Reviewed
• [Journal Article] Expression of angiogenic factors in rat periapical lesions (2012)
  • Author(s): Yamanaka Y, Kaneko T, Yoshiba K, Kaneko R, Yoshiba N, Shigetani Y, NOr JE, Okiji T
  • Journal Title: Journal of Endodontics Volume: 38(3) Pages: 313-317
  • Peer Reviewed
• [Journal Article] Gene Expression analysis of membrane transport proteins in normal and lipopolysaccharide-inflamed rat dental pulp (2012)
  • Author(s): Ohkura N, Shigetani Y, Yoshiba N, Yoshiba K, Okiji T
  • Journal Title: Journal of Endodontics Volume: 38(5) Pages: 648-652
  • Peer Reviewed
• [Journal Article] Immunohistochemical analysis of two stem cell markers of α-smooth muscle actin and STRO-1 during wound healing of human dental pulp (2012)
  • Author(s): Yoshiba N, Yoshiba K, Ohkura N, Shigetani Y, Takei E, Hosoya A, Nakamura H, Okiji T
  • Journal Title: Histochem Cell Biol Volume: 138(4) Pages: 583-592
  • Peer Reviewed
• [Journal Article] Odontoblast response to cavitypreparation with Er:YAG laser in ratmolars: an immunohistochemicalstudy (2012)
  • Author(s): Shigetani Y, Suzuki H, Ohshima H,Yoshiba K, Yoshiba N, Okiji T
  • Journal Title: Odontology Epub 2012 Jun 27 Volume: 101(2) Issue: 2 Pages: 186-192
  • DOI: 10.1007/s10266-012-0078-x
  • Peer Reviewed
• [Journal Article] Gene expression analysis of membrane transport proteins in normal and lipopolysaccharide-inflamed rat dental pulp (2012)
  • Author(s): Ohkura N, Shigetani Y, Yoshiba N, Yoshiba K, Okiji T
  • Journal Title: J Endod Volume: 38(5) Issue: 5 Pages: 648-652
  • DOI: 10.1016/j.joen.2012.02.012
  • Peer Reviewed
• [Journal Article] Expression of angiogenic factors in rat periapical lesions (2012)
  • Author(s): Yusuke Yamanaka, Tomoatsu Kaneko, Kunihiko Yoshiba, Reika Kaneko, Nagako Yoshiba, Yoshimi Shigetani, Jacques E. Nor, Takashi Okiji
  • Journal Title: Journal of Endodontics Volume: 38(3) Issue: 3 Pages: 313-317
  • DOI: 10.1016/j.joen.2011.11.009
  • Peer Reviewed
• [Journal Article] ニッケルチタンファイルReciprocの彎曲根管切削特性 (2012)
  • Author(s): 日向 剛, 重谷佳見, 吉羽邦彦, 興地隆史
  • Journal Title: 日歯保存誌 Volume: 55(6) Pages: 381-388
  • Peer Reviewed
• [Journal Article] GaAlAs Laser Irradiation Induces Active Tertiary Dentin Formation Following Pulpal Apoptosis and CellProliferation in Rat Molars (2011)
  • Author(s): Shigetani Y, Sasa N, Suzuki H, Okiji T, and Ohshima H
  • Journal Title: Journal of Endodontics Volume: 37(8) Pages: 1086-1091
  • Peer Reviewed
• [Journal Article] Immunohistochemical analysis of subcutaneous tissue reactions to methacrylate resin-based root canal sealers (2011)
  • Author(s): Yamanaka Y, Shigetani Y, Yoshiba K, Yoshiba N, Okiji T
  • Journal Title: International Endodontic Journal Volume: 44(7) Pages: 669-675
  • Peer Reviewed
• [Journal Article] GaAlAs Laser Irradiation Induces Active Tertiary Dentin Formation Following Pulpal Apoptosis and Cell Proliferation in Rat Molars (2011)
  • Author(s): Shigetani Y, Sasa N, Suzuki H, Okiji T, Ohshima H
  • Journal Title: Journal of Endodontics Volume: 37(8) Pages: 1086-1091
  • Peer Reviewed
• [Presentation] Multidrug resistance-associated protein 4-mediated prostaglandin E2-transport in inflamed dental pulp (2013)
  • Author(s): Ohkura N, Shigetani Y, Yoshiba N, Yoshiba K, Okiji T
  • Organizer: 91st General Session & Exhibition of the International Association for Dental Research (IADR)
  • Place of Presentation: Seattle, WA, USA
  • Year and Date: 2013-03-21
• [Presentation] Distributional changes of macrophage-subpopulations in rat molars pulp-capped with MTA (2013)
  • Author(s): Takei E, Shigetani Y, Yoshiba K, Hinata G, Yoshiba N, Okiji T
  • Organizer: 91st General Session & Exhibition of the International Association for Dental Research (IADR)
  • Place of Presentation: Seattle, WA, USA
  • Year and Date: 2013-03-21
• [Presentation] Ultrastructural and compositional changes of MTA implanted in connective tissue (2013)
  • Author(s): Yoshiba K, Shigetani Y, Yamanaka Y, Takei E, Yoshiba N, Okiji T
  • Organizer: 91st General Session & Exhibition of the International Association for Dental Research (IADR)
  • Place of Presentation: Seattle, WA, USA
  • Year and Date: 2013-03-21
• [Presentation] ラット臼歯におけるMineral Trioxide Aggregateによる直接覆髄後のM2マクロファージの動態 (2013)
  • Author(s): 武井絵梨花、重谷佳見、吉羽邦彦、日向 剛、吉羽永子、興地隆史
  • Organizer: 日本歯科保存学会
  • Place of Presentation: 秋田県総合生活文化会館
• [Presentation] Distributional changes of macrophage-subpopulations in rat molars pulp-capped with MTA (2013)
  • Author(s): Takei E, Shigetani Y, Yoshiba K, Hinata G, Yoshiba N, Okiji T
  • Organizer: IADR
  • Place of Presentation: Seattle, WA, USA
• [Presentation] Multidrug resistance-associated protein 4-mediated prostaglandin E2-transport in inflamed dental pulp (2013)
  • Author(s): Ohkura N, Shigetani Y, Yoshiba N, Yoshiba K, Okiji T
  • Organizer: IADR
  • Place of Presentation: Seattle, WA, USA
• [Presentation] Alpha-SMA and STRO-1 during wound healing of human dental pulp (2013)
  • Author(s): Yoshiba N, Yoshiba K, Ohkura N, Shigetani Y, Takei E, Hosoya A, Nakamura H, Okiji T
  • Organizer: IADR
  • Place of Presentation: Seattle, WA, USA
• [Presentation] Morphological and compositional changes of MTA implanted in rat connective tissue (2012)
  • Author(s): Yoshiba K, Shigetani Y, Yamanaka Y, Takei E, Yoshiba N, Okiji T
  • Organizer: Japan-China Dental Conference 2012
  • Place of Presentation: 成都, 中国
• [Presentation] Canal-shaping ability of a Ni-Ti instrument for single-file reciprocating technique (2012)
  • Author(s): Hinata G, Shigetani Y, Yoshiba K, Okiji T
  • Organizer: 60th Annual Meeting of JADR
  • Place of Presentation: Niigata(Program and abstracts)
• [Presentation] ラット臼歯におけるMineral Trioxide Aggregate による直接覆髄後のマクロファージ系細胞の動態解析 (2012)
  • Author(s): 武井絵梨花, 重谷佳見, 吉羽邦彦, 日向剛, 吉羽永子, 興地隆史
  • Organizer: 日本歯科保存学会2012年度春季学術大会(第136回)
  • Place of Presentation: 沖縄(プログラムおよび講演抄録集)
• [Presentation] 半導体レーザー照射後のラット臼歯における硬組織形成誘導機構の解明 (2012)
  • Author(s): 重谷佳見, 大倉直人, 細矢明宏, 鈴木啓展, 吉羽邦彦, 吉羽永子, 大島勇人, 興地隆史
  • Organizer: 第22回日本歯科医学会総会
  • Place of Presentation: 大阪(プログラム・事前抄録集)
• [Presentation] Morphological and compositional changes of MTA implanted in rat connective tissue (2012)
  • Author(s): Yoshiba K, Shigetani Y, Yamanaka Y, Takei E, Yoshiba N, Okiji T
  • Organizer: Japan-China Dental Conference 2012
  • Place of Presentation: Seito, Caina
• [Presentation] Macrophage/dendritic cell responses to pulp-capping materials in rat subcutaneous tissue (2012)
  • Author(s): Ito T, Kaneko T, Yamanaka Y, Yoshiba K, Yoshiba N, Shigetani Y, Okiji T
  • Organizer: JADR
  • Place of Presentation: Niigata, Japan
• [Presentation] Canal-shaping ability of a Ni-Ti instrument for single-file reciprocating technique (2012)
  • Author(s): Hinata G, Shigetani Y, Yoshiba K, Okiji T
  • Organizer: JADR
  • Place of Presentation: Niigata, Japan
• [Presentation] ラット実験的根尖歯周炎の成立過程における血管新生関連遺伝子の発現 (2012)
  • Author(s): 山中 裕介, 金子 友厚, 吉羽 邦彦, 伊藤 崇史, 吉羽 永子, 重谷 佳見, 興地 隆史
  • Organizer: 新潟歯学会
  • Place of Presentation: 新潟
• [Presentation] Er:YAGレーザーによる窩洞形成後のラット臼歯歯髄反応に関する免疫組織学的検討 (2012)
  • Author(s): 重谷佳見, 吉羽邦彦, 吉羽永子, 興地隆史
  • Organizer: 日本歯内療法学会
  • Place of Presentation: 東京
• [Presentation] 試作S-PRGフィラー含有根管充填用シーラーの生体機能性について (2012)
  • Author(s): 韓 臨麟, 吉羽 邦彦, 重谷 佳見, 武井 絵梨花, 吉羽 永子, 興地 隆史
  • Organizer: 日本歯内療法学会
  • Place of Presentation: 東京
• [Presentation] ラット臼歯におけるMineral Trioxide Aggregateによる直接覆髄後のマクロファージ系細胞の動態解析 (2012)
  • Author(s): 武井 絵梨花, 重谷 佳見, 吉羽 邦彦, 日向 剛, 吉羽 永子, 興地 隆史
  • Organizer: 日本歯科保存学会
  • Place of Presentation: 沖縄
• [Presentation] ラット炎症歯髄に対する薬物輸送担体の遺伝子発現解析 (2012)
  • Author(s): 大倉直人, 重谷佳見, 細矢明宏, 吉羽永子, 吉羽邦彦, 興地隆史
  • Organizer: 歯科基礎医学会
  • Place of Presentation: 郡山
• [Presentation] 半導体レーザー照射後のラット臼歯における硬組織形成誘導機構の解明 (2012)
  • Author(s): 重谷佳見, 大倉直人, 細矢明宏, 鈴木啓展, 吉羽邦彦, 吉羽永子, 大島勇人, 興地隆史
  • Organizer: 日本歯科医学会総会
  • Place of Presentation: 大阪
• [Presentation] ラット炎症歯髄に対する薬物輸送担体の遺伝子発現解析 (2012)
  • Author(s): 大倉直人, 重谷佳見, 細矢明宏, 吉羽永子, 吉羽邦彦, 興地隆史
  • Organizer: 日本歯科医学会
  • Place of Presentation: 大阪
• [Presentation] ラット臼歯における水酸化カルシウムによる直接覆髄後の被蓋硬組織形成過程に関する免疫組織化学的研究 (2011)
  • Author(s): 重谷佳見、鞍立桃子、吉羽邦彦、吉羽永子、山中裕介、興地隆史
  • Organizer: 日本歯科保存学会2011年秋季学術大会(第135回)
  • Place of Presentation: 大阪(日歯保存誌, プログラムおよび講演抄録集)
  • Year and Date: 2011-10-20
• [Presentation] Mineral Trioxide Aggregate の生体内組成変化 : ラット皮下移植による検討 (2011)
  • Author(s): 吉羽邦彦, 重谷佳見, 山中裕介, 武井絵梨花, 吉羽永子, 興地隆史
  • Organizer: 日本歯科保存学会2011年度秋季学術大会(第135回)
  • Place of Presentation: 大阪(プログラムおよび講演抄録集)
  • Year and Date: 2011-10-20
• [Presentation] Compositional changes of mineral trioxide aggregate subcutaneously implanted in rats (2011)
  • Author(s): Yoshiba K, Shigetani Y, Yamanaka Y, Takei E, Yoshiba N, Okiji T
  • Organizer: 59th Annual Meeting of Japanese Association of Dental Rsearch
  • Place of Presentation: Hiroshima(Program and Abstracts of Papers)
  • Year and Date: 2011-10-08
• [Presentation] ラット臼歯における水酸化カルシウムによる直接覆髄後の被蓋硬組織形成過程に関する免疫組織化学的研究 (2011)
  • Author(s): 重谷佳見、鞍立桃子、吉羽邦彦、吉羽永子、山中裕介、興地隆史
  • Organizer: 日本歯科保存学会
  • Place of Presentation: 大阪国際交流センター（大阪）
• [Remarks] 新潟大学大学院医歯学総合研究科口腔健康科学講座う蝕学分野
  • URL: http://www.dent.niigata-u.ac.jp/restend/restend.html
• [Remarks] 新潟大学大学院医歯学総合研究科口腔健康科学講座う蝕学分野
  • URL: http://www.dent.niigata-u.ac.jp/restend/restend.html