Effects of degraded dentin matrix proteins on the repair process of dentin-pulp complex

• Project/Area Number: 23792170
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Conservative dentistry
• Research Institution: Osaka University
• Principal Investigator: TAKAHASHI Yusuke 大阪大学, 大学院・歯学研究科, 助教 (60397693)
• Project Period (FY): 2011 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2011: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
• Keywords: 歯髄 / 象牙質 / 修復象牙質 / 第三象牙質 / 象牙質-歯髄複合体 / MMP / 歯学

Research Abstract

The objective of this study was to elucidate the wound healing mechanism of dentin-pulp complex. We investigated the effects of degraded dentin matrix proteins (DMPs) induced by acids or proteinases on the function of pulp cells. External acid or internal MMP molecules were found to degrade DMPs and pulp cells were promoted their proliferation or differentiation by degraded DMPs. These results indicated that degraded DMPs potentially affected the healing process of dentin-pulp complex.

Research Products

• [Journal Article] Activation of the Wnt/β-catenin pathway and tissue inhibitor of metalloprotease 1 during tertiary dentinogenesis (2013)
  • Author(s): Yoshioka S
  • Journal Title: Journal of Biochemistry Volume: 153 Issue: 1 Pages: 43-50
  • DOI: 10.1093/jb/mvs117
  • Peer Reviewed
• [Journal Article] う蝕象牙質除去後の残存細菌にEr: YAGレーザーが与える影響 (2013)
  • Author(s): 高橋雄介, 吉岡靖介, 朝日陽子, 永山智崇, 野杁由一郎, 林 美加子
  • Journal Title: 日本歯科保存学雑誌 Volume: 第56巻 Pages: 1-8
  • Peer Reviewed
• [Journal Article] う蝕象牙質除去後の残存細菌にEr: YAGレーザーが与える影響 (2013)
  • Author(s): 高橋雄介, 吉岡靖介, 朝日陽子, 永山智崇, 野杁由一郎, 林 美加子
  • Journal Title: 日本歯科保存学雑誌 Volume: 56 Pages: 1-8
  • Peer Reviewed
• [Journal Article] Effects of the antibacterial monomer 12-methacryloyloxydodecylpyridinium bromide (MDPB) on bacterial viability and metabolism (2011)
  • Author(s): Izutani N, Imazato S, Nakajo K, Takahashi N, Takahashi Y, Ebisu S, Russell RRB
  • Journal Title: Eur J Oral Sci Volume: 119(2) Issue: 2 Pages: 175-181
  • DOI: 10.1111/j.1600-0722.2011.00817.x
  • Peer Reviewed
• [Presentation] Effects of degraded dentin matrix proteins on pulp cellsi function (2013)
  • Author(s): Takahashi Y, Okamoto M, Yoshioka S, Hayashi M
  • Organizer: IADR Pulp Biology and Regeneration Group Symposium 2013
  • Place of Presentation: サンフランシスコ、米国
  • Year and Date: 2013-03-26
• [Presentation] Effect of degraded dentin matrix proteins on pulp cell function (2013)
  • Author(s): Okamoto M, Takahashi Y, Yoshioka S, Hayashi M
  • Organizer: 91st General Session & Exhibirion of the IADR
  • Place of Presentation: シアトル、米国
  • Year and Date: 2013-03-23
• [Presentation] Effect of degraded dentin matrix proteins on pulp cell function (2013)
  • Author(s): Okamoto M, Takahashi Y, Yoshioka S, Hayashi M
  • Organizer: 91st General Session & Exhibirion of the IADR
  • Place of Presentation: Washington State Convention Center (シアトル、米国)
• [Presentation] Effects of degraded dentin matrix proteins on pulp cells’ function (2013)
  • Author(s): Takahashi Y, Okamoto M, Yoshioka S, Hayashi M
  • Organizer: IADR Pulp Biology and Regeneration Group Symposium 2013
  • Place of Presentation: Arthur A. Dugoni School of Dentistry (サンフランシスコ、米国)
• [Presentation] Effect of Degraded Dentin Matrix Proteins induced by Matrix Metalloproteinase Molecules on Pulp Cells Function (2012)
  • Author(s): Takahashi Y, Okamoto M, Yoshioka S, Hayashi M
  • Organizer: 日本歯科保存学会 2012年度秋季学術大会(第137回)
  • Place of Presentation: 広島市
  • Year and Date: 2012-11-22
• [Presentation] Analysis of pulpal gene expression during tertiary Takahashi Y dentin formation (2012)
  • Author(s): Takahashi Y
  • Organizer: Japan-Korea Joint Symposium Development and Regeneration of Oro-Facial Structures(招待講演)
  • Place of Presentation: 吹田市
  • Year and Date: 2012-07-13
• [Presentation] Effect of Degraded Dentin Matrix Proteins induced by Matrix Metalloproteinase Molecules on Pulp Cells Function (2012)
  • Author(s): Takahashi Y, Okamoto M, Yoshioka S, Hayashi M
  • Organizer: 第137回日本歯科保存学会 2012年度秋季学術大会
  • Place of Presentation: 広島国際会議場（広島県）
• [Presentation] Analysis of pulpal gene expression for designing pulp capping materials (2011)
  • Author(s): Takahashi Y
  • Organizer: Start-up symposium for innovative materials research "A Roadmap for the Future of Oral Biomaterials"(招待講演)
  • Place of Presentation: 吹田市
  • Year and Date: 2011-07-03
• [Presentation] 修復象牙質形成過程におけるTissue inhibitor of metalloprotease 1の発現誘導 (2011)
  • Author(s): 吉岡靖介、高橋雄介、今里 聡、恵比須繁之
  • Organizer: 日本歯科保存学会2011年度春季学術大会(第134回)
  • Place of Presentation: 浦安市
  • Year and Date: 2011-06-09
• [Presentation] 修復象牙質形成過程におけるTissue inhibitor of metalloprotease 1の発現誘導 (2011)
  • Author(s): 吉岡靖介、高橋雄介、今里聡、恵比須繁之
  • Organizer: 日本歯科保存学会2011年度春季学術大会（第134回）
  • Place of Presentation: 東京ベイ舞浜ホテルクラブリゾート
• [Presentation] Analysis of pulpal gene expression for designing pulp capping materials (2011)
  • Author(s): Yusuke Takahashi
  • Organizer: Start-up symposium for innovative materials research "A Roadmap for the Future of Oral Biomaterials"(招待講演)
  • Place of Presentation: 大阪大学
• [Presentation] Analysis of pulpal gene expression during tertiary dentin formation
  • Author(s): Takahashi Y
  • Organizer: Japan-Korea Joint Symposium“Development and Regeneration of Oro-Facial Structures”
  • Place of Presentation: 大阪大学（大阪府）
  • Invited
• [Book] Advances in Medicine and Biology (2011)
  • Author(s): Yusuke Takahashi, Anthony J. Smith, Paul R. Cooper
  • Total Pages: 335
  • Publisher: Nova Science Publishers
• [Remarks] 大阪大学大学院歯学研究科 口腔分子感染制御学講座(歯科保存学教室)ホームページ
  • URL: http://www.dent.osaka-u.ac.jp/english/g_school/facilities/endodontology.html
• [Remarks] 大阪大学大学院歯学研究科 口腔分子感染制御学講座（歯科保存学教室）ホームページ
  • URL: http://www.dent.osaka-u.ac.jp/english/g_school/facilities/endodontology.html