The generation of ameloblasts from adult tissue-delivered cells and the method of the tooth germ regenration

• Project/Area Number: 23792176
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Conservative dentistry
• Research Institution: Hiroshima University
• Principal Investigator: SUZUKI Shigeki 広島大学, 医歯薬保健学研究院, 助教 (30549762)
• Project Period (FY): 2011 – 2013
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2013: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2012: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000); Fiscal Year 2011: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
• Keywords: 象牙芽細胞 / DMP-1 / DSPP / 保存修復 / 口腔粘膜上皮

Research Abstract

The original main purpose of this research project was the identification of the factors that induce epithelial-mesenchymal interaction during tooth development. However, it was difficult to isolate and cultivate oral epithelial cells. Since it has been suggested that the enamel and dentin extracellular matrix (ECM) proteins would be able to regulate ameloblasts and odontoblasts differentiation in vitro, we generated the recombinant proteins of tooth ECM proteins and analyzed their effects for mesenchymal stem cells (MSCs) adhesion and differentiation. DMP-1 was able to induce MSCs adhesion and differentiation into osteogenic lineage cells while intact DPP was unable to. Thus, the functions of these proteins may be modulated by proteolytic cleavages by unknown extracellular proteases.

Research Products

• [Journal Article] The Inflammation-lipocalin2 axis may contribute to the development of chronic kidney disease (2014)
  • Author(s): Hashikata, A., Yamashita, A., Suzuki, S., Nagayasu, S., Shinjo, T., Taniguchi, A., Fukushima, M., Nakai, Y., Nin, K., Watanabe, N., Asano, T., Abiko, Y., Kushiyama, A., Nagasaka, S., and Nishimura, F.
  • Journal Title: Nephrol Dial Transplant Volume: 29(3) Pages: 611-618
  • Peer Reviewed
• [Journal Article] The Inflammation-lipocalin2 axis may contribute to the development of chronic kidney disease. (2014)
  • Author(s): Hashikata, A., Yamashita, A., Suzuki, S., Nagayasu, S., Shinjo, T., Taniguchi, A., Fukushima, M., Nakai, Y., Nin, K., Watanabe, N., Asano, T., Abiko, Y., Kushiyama, A., Nagasaka, S., and *Nishimura, F.
  • Journal Title: Nephrol Dial Transplant. Volume: 29 Pages: 611-618
  • Peer Reviewed
• [Journal Article] Lessons Learned from Mouse Models of DSPP, DSP, and DPP (2012)
  • Author(s): Suzuki, S., and Kulkarni, A. B.
  • Journal Title: Bentham Science Publishers Volume: 10 Pages: 221-230
• [Journal Article] Dentin sialophosphoprotein and dentin matrix protein-1 : Two highly phosphorylated proteins in mineralized tissues (2012)
  • Author(s): Suzuki, S., Haruyama, N., Nishimura, F., and Kulkarni, A. B.
  • Journal Title: Arch Oral Biol Volume: 57(9) Pages: 1165-1175
  • Peer Reviewed
• [Journal Article] Smoking and adipose tissue inflammation suppress leptin expression in Japanese obese males : Potential mechanism of resistance to weight loss among Japanese obese smokers (2012)
  • Author(s): Nagayasu S, Suzuki S, Yamashita A, Taniguchi A, Fukushima M, Nakai Y, Nin K, Watanabe N, Nagasaka S, Yabe D, Nishimura F
  • Journal Title: Tob Induc Dis Volume: 10 Issue: 1 Pages: 3-3
  • DOI: 10.1186/1617-9625-10-3
  • Peer Reviewed
• [Journal Article] Dentin sialophosphoprotein and dentin matrix protein-1: Two highly phosphorylated proteins in mineralized tissues. (2012)
  • Author(s): Suzuki S, Haruyama N, Nishimura F, Kulkarni AB.
  • Journal Title: Arch Oral Biol. Volume: 57（9） Pages: 1165-75
  • Peer Reviewed
• [Journal Article] Dentin Sialophosphoprotein and Dentin Matrix Protein-1: Two Highly Phosphorylated Proteins in Mineralized Tissues (2012)
  • Author(s): Shigeki Suzuki, Naoto Haruyama, Fusanori Nishimura, and Ashok B. Kulkarni
  • Journal Title: Archives of Oral Biology Volume: In Press
  • Peer Reviewed
• [Presentation] 内在性Wntによる歯髄創傷治癒促進効果とDentin Phosphoproteinによる硬組織形成作用の検討 (2013)
  • Author(s): 鈴木茂樹
  • Organizer: 第138回日本歯科保存学会シンポジウムI「若手研究者が描くPulp Wound Healing & Regeneration」
  • Place of Presentation: 福岡国際会議場
  • Year and Date: 2013-06-27
• [Presentation] 内在性Wntによる歯髄創傷治癒促進効果とDentin Phosphoproteinによる硬組織形成作用の検討 (2013)
  • Author(s): 鈴木茂樹
  • Organizer: 日本歯科保存学会
  • Place of Presentation: 福岡
  • Invited
• [Presentation] Repeat sequence 改変型およびRGD配列改変型 Dentin sialophosphoprotein発現株の作製 (2012)
  • Author(s): 小武家 誠司, 鈴木 茂樹, 西村 英紀
  • Organizer: 日本歯科保存学会
  • Place of Presentation: 沖縄
• [Presentation] Dentin sialophosphoproteinの開裂がその機能発現に及ぼす影響の検討 (2012)
  • Author(s): 小武家誠司、鈴木茂樹、藤井理史、西村英紀
  • Organizer: 日本歯科保存学会
  • Place of Presentation: 広島
• [Presentation] 内在性Wntによる歯髄創傷治癒促進効果とDentin Phosphoproteinによる硬組織形成作用の検討
  • Author(s): 鈴木茂樹
  • Organizer: 日本歯科保存学会
  • Place of Presentation: 福岡国際会議場
  • Invited
• [Book] Lessons Learned from Mouse Models of DSPP, DSP, and DPP (2012)
  • Author(s): Shigeki Suzuki and Ashok Kulkarni
  • Total Pages: 10
  • Publisher: Bentham Science Publishers