| Project/Area Number |
23792240
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Prosthetic dentistry
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| Research Institution | Kagoshima University |
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Principal Investigator |
KASAI Takayuki 鹿児島大学, 大学院・医歯学総合研究科, 助教 (30457650)
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| Project Period (FY) |
2011 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2011: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
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| Keywords | AMPK / 骨芽細胞 / メカニカルストレス / シグナル伝達 / LIPUS / 細胞分化 |
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| Research Abstract |
AMP-activated protein kinase(AMPK) is a kinase responds to mechanical stress. Phosphorylation of AMPKαwas significantly decreased during osteoblastic differentiation in both primary osteoblasts and MC3T3-E1, a mouse osteoblastic cell line. Conversely, the terminal differentiation of primary osteoblasts and MC3T3-E1 cells, represented by matrix mineralization, was significantly inhibited by stimulation with metformin, an activator of AMPK. Matrix mineralization of MC3T3-E1 cells was also inhibited by the forced expression of a constitutively active form of AMPKα. Metformin significantly inhibited gene expression of Runx2 along with osteoblast differentiation markers. Low-intensity pulsed ultrasound (LIPUS), which is used clinically to promote bone fracture healing, decreased AMPKαphosphorylation. Thus, LIPUS may promote osteoblast differentiation through dephosphorylation of AMPK.
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