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Development of cultured cell sheet and nano-coating scaffold for periodontal regeneration

Research Project

Project/Area Number 23792267
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Dental engineering/Regenerative dentistry
Research InstitutionHokkaido University

Principal Investigator

SHIMOJI Shinji  北海道大学, 歯学研究科(研究院), 助教 (30431373)

Project Period (FY) 2011 – 2013
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
Fiscal Year 2013: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2011: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Keywords歯周組織再生 / 培養骨髄細胞シート / コラーゲンスキャホールド / 培養細胞シート / 骨再生 / 線維芽細胞増殖因子 / ナノコーティングスキャホールド
Research Abstract

The purpose of the present study was determine: (1) the novel method of cultured cell sheet using bone marrow cell from F344/Jcl rats' femur; (2) the effectiveness of the cultured bone marrow cell sheet, FGF-2 and collagen sponge scaffold on bone regeneration of rats' calvariae.
The results were as follows: (1) the multilayered osteoblast-like cell sheet was produced by this novel method. The proper culture term made this cell sheet more rigid; (2) the complex of cultured bone marrow cell sheet, FGF-2 and collagen sponge scaffold made large amount of new bone on rats' calvariae.

Report

(4 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Research-status Report
  • 2011 Research-status Report
  • Research Products

    (5 results)

All 2012

All Journal Article (2 results) (of which Peer Reviewed: 1 results) Presentation (3 results)

  • [Journal Article] Bone-orchestrating cells, osteocytes2012

    • Author(s)
      Shimoji S, et al他9名
    • Journal Title

      Hokkaido J Dent Sci

      Volume: 32 Pages: 93-103

    • Related Report
      2013 Final Research Report
  • [Journal Article] Hongo H, Hasegawa T, Sasaki M, Suzuki R, Masuki H, Yamada T, Shimoji S, Kawanami M, Yamamoto T, Amizuka N2012

    • Author(s)
      Bone-orchestrating cells, osteocytes.
    • Journal Title

      Hokkaido Journal of Dental Science

      Volume: 32 Pages: 93-103

    • Related Report
      2011 Research-status Report
    • Peer Reviewed
  • [Presentation] Histochemical examination on osteocytes and their lacunae after administration of parathyroid hormone in mice2012

    • Author(s)
      Shimoji S, et al
    • Organizer
      22^<nd> ANZBMS Annual scientific meeting with 1^<st> Asia-Pacific Bone & Mineral Research Meeting
    • Place of Presentation
      Pan Pacific Perth Hotel, Perth, Australia
    • Year and Date
      2012-09-05
    • Related Report
      2013 Final Research Report
  • [Presentation] Histochemical examination on osteocytes and their lacunae after administration of parathroid hormone in mice.2012

    • Author(s)
      Hongo H, Sasaki M, Yamada T, Hasegawa T, Yamamoto T, Nakano T, Shimoji S, Amizuka N
    • Organizer
      22nd ANZBMS Annual scientific meeting with 1st Asia-Pacific Bone & Mineral Research Meeting
    • Place of Presentation
      Pan Pacific Perth Hotel (Australia)
    • Related Report
      2012 Research-status Report
  • [Presentation] 副甲状腺ホルモン投与による骨細胞性骨融解に関する組織化学的検索.2012

    • Author(s)
      本郷裕美、佐々木宗輝、長谷川智香、鈴木礼子、下地伸司、網塚憲生
    • Organizer
      第117回日本解剖学会総会・全国大会
    • Place of Presentation
      山梨大学甲府キャンパス(甲府市)
    • Related Report
      2011 Research-status Report

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Published: 2011-08-05   Modified: 2019-07-29  

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