Effect of messencymal stem cells proliferation and differentiationon self-assembled monolayer using serum-free medium

• Project/Area Number: 23792282
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Dental engineering/Regenerative dentistry
• Research Institution: Hiroshima University
• Principal Investigator: HIRATA Isao 広島大学, 医歯薬保健学研究院, 助教 (40346507)
• Project Period (FY): 2011 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2011: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
• Keywords: 再生医学 / 表面・界面物性、細胞・組織 / 間葉系幹細胞 / 自己組織化単分子膜 / 表面･界面物性 / 細胞･組織 / 幹葉系幹細胞 / 無血清培地 / SAM / MSC

Research Abstract

The aim of this study is fabrication of chemically-defined cell culture system using a mixed SAM substrate and a serum-free culture medium. By combining a serum-free culture medium and culture plate having a suitablechemical composition, the present culture system, which it is possible to improve theculture efficiency of such proliferation and differentiation, leading to significant advances in regenerative medicine and cell biology.

Research Products

• [Presentation] Evaluation between Surface Chemical Composition and Cell Proliferation using Mixed Self-Assembled Monolayers (2012)
  • Author(s): Hirata I., Saskianti T., Kanawa M., Kawamoto T., Kato K., Kato Y.
  • Organizer: 9th World Biomaterials Congress
  • Place of Presentation: Chengdu China
• [Presentation] 培養環境全体の完全化学規定化:臨床で有用な高性能ヒト幹細胞の供給 (2012)
  • Author(s): 加藤幸夫,平田伊佐雄
  • Organizer: BIOtech 2012: 第11回 国際バイオテクノロジー展/技術会議
  • Place of Presentation: 東京
• [Presentation] 化学的に規定された培養液と培養基板での幹葉系幹細胞の増殖の向上 (2012)
  • Author(s): 平田伊佐雄,Tania Saskianti,金輪真佐美,河本 健,加藤功一,加藤幸夫
  • Organizer: 第25回日本軟骨代謝学会
  • Place of Presentation: 名古屋
• [Presentation] Evaluation between Surface Chemical Composition and Cell Proliferation using Mixed Self-Assembled Monolayers (2012)
  • Author(s): Hirata I., Saskianti T., Kanawa M., Kawamoto T., Kato K., Kato Y.
  • Organizer: 9th World Biomaterials Congress
  • Place of Presentation: 成都（中華人民共和国）
• [Presentation] 化学的に規定された培養液と培養基板での幹葉系幹細胞の増殖の向上 (2012)
  • Author(s): 平田伊佐雄、Tania Saskianti、金輪真佐美、河本 健、加藤功一、加藤幸夫
  • Organizer: 第25回日本軟骨代謝学会
  • Place of Presentation: ウィルあいち