Development of microinjection method of forming at initiation sage of tooth.

• Project/Area Number: 23792299
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Dental engineering/Regenerative dentistry
• Research Institution: The Nippon Dental University
• Principal Investigator: TSUTSUI Takeo 日本歯科大学, 生命歯学部, 准教授 (70366764)
• Project Period (FY): 2011 – 2013
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2012: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2011: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
• Keywords: 歯髄幹細胞 / マイクロインジェクション / 口腔粘膜上皮 / 口腔粘膜上皮幹細胞 / 三次元共培養 / マイクロインジェクション法 / 上皮間葉相互作用 / 三次培養

Research Abstract

Dental pulp stem cells and oral epithelial stem cells cultured in temperature dependent dish for form cell sheet. The cell sheets removed to collagen gel on culture dish for a three dimensional culture. Three dimensional culture prepared for microinjection. Histological analysis showed cells accumulation on basal cells layer of oral epithelial cells. The cells accumulation occurred contacted with dental pulp stem cells that observed epithelial cells invasion-like histology. Three dimensional culture method of dental pulp stem cells and oral epithelial cells may indicated that interaction of epithelial cells and mesenchymal cells.