Analysis of supporting abilities for undifferentiated dental pulp stem cells by their microenvironments
Project/Area Number |
23792304
|
Research Category |
Grant-in-Aid for Young Scientists (B)
|
Allocation Type | Multi-year Fund |
Research Field |
Dental engineering/Regenerative dentistry
|
Research Institution | Kansai Medical University |
Principal Investigator |
|
Project Period (FY) |
2011 – 2013
|
Project Status |
Completed (Fiscal Year 2013)
|
Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2013: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2012: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2011: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
|
Keywords | 歯髄幹細胞 / 細胞外環境 / 低酸素 / 造血細胞支持 / E-cadherin / 上皮細胞 / 間葉細胞 / 造血制御 / 再生歯学 |
Research Abstract |
The expressions of mRNA and cytokines of PDGFRalpha and Sca-1 double positive dental pulp stem cells (DPSCs) were compared to bone marrow-derived mesenchymal stem/stromal cells (BM-MSCs). These mRNA and cytokines expressions did not show significant difference between DPSCs and BM-MSCs except Cxcl12 mRNA expression. We investigated whether proliferation and differentiation potentials of passaged DPSCs are improved by hypoxic culture. The DPSCs well maintained a proliferation and differentiation potentials with passage under the hypoxic culture condition. These results suggest that the proliferation and differentiation potentials of DPSCs were maintained in hypoxic condition. Then, hematopoietic stem/progenitor cells (HSPCs) supporting activity of DPSCs was analyzed by in vitro co-culture system. Moreover, their HSC-supporting activity was evaluated by in vivo xenotransplantation assays using NOG mice. The DPSCs as well as BM-MSCs supported human cord blood-derived HSCs.
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Report
(4 results)
Research Products
(29 results)