The analysis of expression state of HOX family genes and their effects for cancer-associated in oral cancer

• Project/Area Number: 23792317
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Surgical dentistry
• Research Institution: Chiba University
• Principal Investigator: KOUZU Yukinao 千葉大学, 大学院・医学研究院, 助教 (70400942)
• Project Period (FY): 2011 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2012: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000); Fiscal Year 2011: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: HOX family / HOXA1 / 口腔癌 / マイクロアレイ / siRNA / ホメオティック遺伝子 / HOX遺伝子family

Research Abstract

The purpose of this study was to examine the potential relationship of oral squamous cell carcinoma (OSCC) and HOX family which is transcription factors. We evaluated the status of HOX genes mRNA expression in OSCC cell lines by real-time PCR. The expression of HOXA1 and oral cancer related genes were particularly increased in all OSCC-derived cell lines. Similar to cell lines, increased expression was observed at mRNA and protein levels in clinical specimens. In addition, suppression of HOXA1 expression with siRNA significantly inhibited cell proliferation compared with the control cells. We suggested that HOXA1 and oral cancer related gene are closely related to OSCC and HOXA1 may play an important role in tumor progression.