|Budget Amount *help
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2012: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2011: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
We used B88 cell and CAL27 cell line which were the culture human oral squamous cell carcinoma cell line which we established in our classroom. Cancer cells to use in this study confirmed that a cell proliferation was inhibited by DOC and we gave DOC 1,10,100,1000pg/ml and sequential level and cultured both cells and manufactured cell line indicating the resistance in DOC. Both cells raise the density of DOC and treat it, and the cancer cells indicating the DOC resistance are establishing it than parent cells.