| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2013: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2012: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2011: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Research Abstract |
Lymphangiogenesis is controlled primarily by VEGF receptor signaling and its signaling is enhanced by reverse signaling from Ephrin-B2. We determined whether Ephrin-B2 expressed in oral squamous cell carcinoma contributes to lymph node metastasis. Introduction of Ephrin-B2-siRNA into SAS-L1 cells significantly suppressed adhesion and cell-to-cell migration activities to hLEC cells. In a model constructed by implanting SAS-L1 cells in the tongue of SCID mice, injection of Ephrin-B2-siRNA significantly reduced the number of cervical lymph nodes with metastasis. Ephrin-B2 expression level correlated with N classification, tumor grading, and invasion mode of primary lesions from 50 patients with oral squamous cell carcinoma. In summary, Ephrin-B2 signaling was shown to facilitate lymph node metastasis via effects on adhesion to the surrounding lymphatic vessels and migration activities.
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