Research Project
Grant-in-Aid for Young Scientists (B)
I focused microRNA-21 (miR-21) targeting EpCAM as a molecularrelated tumor invasion. To determine the miR-21 target, I searched for molecular gene that involved in tumor invasion by using commonly cited prediction program miRanda. In oral tongue squamous cell carcinoma (OTSCC) cell line, SCC25 cells, I further evaluated whether miR-21 contributes to cell invasiveness by blocking its expression with a specific knock down LNA probe and validated direct target by Matrigel invasion assay and Western blot. I selected DKK2, as a Wnt/antagonist that involved in tumor invasion. miR-21 overexpression was significantly correlated with DKK2-/β-catenin- immunohistochemical phenotype. Knock down of miR-21 significantly decreases the invasion potential of SCC25 cells with up-regulated DKK2. These results suggest that miR-21 may provide potential therapeutic target for OTSCC treatment.
All 2012 2011
All Journal Article (2 results) Presentation (2 results)
International Journal of Oral and Maxillofacial Surgery
Volume: 41 Issue: 10 Pages: 1195-1200
10.1016/j.ijom.2012.07.011
120006985533
Oral Oncology
Volume: 47 Issue: 9 Pages: 855-860
10.1016/j.oraloncology.2011.06.501
120006985032
