Molecular targeting therapy targeted to EpCAM as cancer stem cell marker in oral cancer
Project/Area Number |
23792360
|
Research Category |
Grant-in-Aid for Young Scientists (B)
|
Allocation Type | Multi-year Fund |
Research Field |
Surgical dentistry
|
Research Institution | Nagasaki University |
Principal Investigator |
|
Project Period (FY) |
2011 – 2012
|
Project Status |
Completed (Fiscal Year 2012)
|
Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2011: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
|
Keywords | 口腔癌 / EpCAM / 癌幹細胞 / マイクロRNA / 分子標的治療 |
Research Abstract |
I focused microRNA-21 (miR-21) targeting EpCAM as a molecularrelated tumor invasion. To determine the miR-21 target, I searched for molecular gene that involved in tumor invasion by using commonly cited prediction program miRanda. In oral tongue squamous cell carcinoma (OTSCC) cell line, SCC25 cells, I further evaluated whether miR-21 contributes to cell invasiveness by blocking its expression with a specific knock down LNA probe and validated direct target by Matrigel invasion assay and Western blot. I selected DKK2, as a Wnt/antagonist that involved in tumor invasion. miR-21 overexpression was significantly correlated with DKK2-/β-catenin- immunohistochemical phenotype. Knock down of miR-21 significantly decreases the invasion potential of SCC25 cells with up-regulated DKK2. These results suggest that miR-21 may provide potential therapeutic target for OTSCC treatment.
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Report
(3 results)
Research Products
(4 results)
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[Journal Article] Clinicopathological risk factors for local recurrence in oral squamous cell carcinoma2012
Author(s)
Yanamoto, S., Yamada, S., Takahashi, H., Yoshitomi, I., Kawasaki, G., Ikeda H., Minamizato T., Shiraishi, T., Fujita, S., Ikeda, T., Asahina, I., Umeda, M
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Journal Title
International Journal of Oral and Maxillofacial Surgery
Volume: 41
Issue: 10
Pages: 1195-1200
DOI
NAID
Related Report
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[Journal Article] Isolation and characterization of cancer stem-like side population cells in human oral cancer cells2011
Author(s)
Yanamoto, S., Kawasaki,G.., Yamada, S., Yoshitomi, I., Kawano, T., Yonezawa, H., Rokutanda, S., Naruse, T., Umeda, M
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Journal Title
Oral Oncology
Volume: 47
Issue: 9
Pages: 855-860
DOI
NAID
Related Report
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