Elucidation of the surrounding stromal cells malignant conversion mechanism that parallels the development of cancer

• Project/Area Number: 23792381
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Surgical dentistry
• Research Institution: Keio University
• Principal Investigator: MORIKAWA Satoru 慶應義塾大学, 医学部, 助教 (00424169)
• Project Period (FY): 2011 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2011: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
• Keywords: 間葉系細胞 / 癌周囲間質細胞 / 癌関連線維芽細胞 / 間葉系幹細胞

Research Abstract

In human and mouse cancer tissues, we succeeded in isolating and analysis without culture operation around stromal cells and cancer epithelial cells. This is useful as a method of analyzing surface markers to separate the prospective mesenchymal stem cell

Research Products

• [Journal Article] Primary evaluation of induced pluripotent stem cells using flow cytometry (2013)
  • Author(s): Daisuke Araki
  • Journal Title: Inflammation and Regeneration Volume: 33 Pages: 3-12
  • Peer Reviewed
• [Journal Article] Isolation of mouse mesenchymal stem cells on the basis of expression of Sca-1 and PDGFRα (2012)
  • Author(s): Houlihan DD, Mabuchi Y, Morikawa S, Niibe K, Araki D, Suzuki S, Okano H,Matsuzaki Y.
  • Journal Title: NatureProtocols Volume: 7 Pages: 2103-11
  • Peer Reviewed
• [Journal Article] Isolation of mouse mesenchymal stem cells on the basis of expression of Sca-1 and PDGFR-α (2012)
  • Author(s): Houlihan DD, Mabuchi Y, Morikawa S, Niibe K, Araki D, Suzuki S, Okano H, Matsuzaki Y.(These authors contributed equally to this work.)
  • Journal Title: Nat Protoc Volume: 7(12) Issue: 12 Pages: 2103-2111
  • DOI: 10.1038/nprot.2012.125
  • Peer Reviewed
• [Journal Article] Antibacterial activity of povidone-iodine against an artificial biofilm of Porphyromonas gingivalis and Fusobacterium nucleatum (2012)
  • Author(s): Hosaka, Y., Saito, A., Maeda, R., Fukaya, C., Morikawa, S., Makino, A., Ishihara, K., Nakagawa, T
  • Journal Title: Arch Oral Biol Volume: 57 Issue: 4 Pages: 364-368
  • DOI: 10.1016/j.archoralbio.2011.09.005
  • Peer Reviewed
• [Journal Article] 糖尿病患者における歯周病罹患状態と糖尿病合併症との関係 (2012)
  • Author(s): 太田淳也
  • Journal Title: 日本歯周病学会雑誌 Volume: 54 Pages: 336-345
  • Peer Reviewed
• [Presentation] Prospective Isolation of Dental Pulp Stem Cells (2013)
  • Author(s): Takazumi Yasui
  • Organizer: 91st General Session and Exhibition of the IADR
  • Place of Presentation: Seattle
• [Presentation] LNGFR+Thy-1+CD106high+ cell reveal functionally distinct subpopulations in human mesenchymal stem cells (2013)
  • Author(s): Yo Mabuchi
  • Organizer: 第12回日本再生医療学会総会
  • Place of Presentation: 横浜
• [Presentation] 間葉系幹細胞の体内動態と慢性炎症惹起メカニズム (2013)
  • Author(s): 松崎有未
  • Organizer: 第12回日本再生医療学会総会
  • Place of Presentation: 横浜
• [Presentation] Isolation and functionally distinct mesenchymal stem cells in human bone marrow (2012)
  • Author(s): Satoru Morikawa
  • Organizer: American Academy of periodontology (AAP) 98th ANNUAL MEETING
  • Place of Presentation: Los Angeles, USA
  • Year and Date: 2012-10-02
• [Presentation] LNGFR and Thy-1 based prospective isolation of human mesenchymal stem cells reveal functional distinct subpopulations (2012)
  • Author(s): Yo Mabuchi, Satoru Morikawa
  • Organizer: International Society for Stem Cell Research (ISSCR)
  • Place of Presentation: 10th ANNUAL MEETING Yokohama
  • Year and Date: 2012-06-16
• [Presentation] Isolation of functionally distinct mesenchymal stem cells in human bone marrow (2012)
  • Author(s): Satoru Morikawa
  • Organizer: American Academy of Periodontology (AAP) 98th ANNUAL MEETING
  • Place of Presentation: Los Angeles
• [Presentation] LNGFR and Thy-1 based prospective isolation of human mesenchymal stem cells reveal functional distinct subpopulations (2012)
  • Author(s): Yo Mabuchi
  • Organizer: International Society for Stem Cell Research (ISSCR), 10th ANNUAL MEETING
  • Place of Presentation: Yokohama
• [Presentation] 歯ブラシの大きさの違いによるプラーク除去効果 (2012)
  • Author(s): 森川暁
  • Organizer: 第55回秋季日本歯周病学会学術大会
  • Place of Presentation: つくば
• [Presentation] Single cell isolation elucidates heterogeneity within the human mesenchymal stem/progenitor cell compartment.International Society for Stem Cell Research (ISSCR) (2011)
  • Author(s): Yo Mabuchi, Satoru Morikawa
  • Organizer: 9th ANNUAL MEETING
  • Place of Presentation: Canada
  • Year and Date: 2011-06-18
• [Presentation] 新規歯周組織再生療法確立への挑戦と課題
  • Author(s): 森川暁
  • Organizer: 第2回 日本歯周病学会関東9大学・臨床歯周病学会関東支部合同研修会
  • Place of Presentation: 東京
• [Remarks] 研究グループの紹介
  • URL: http://dent-os.med.keio.ac.jp/KEIO/