Regeneration of the peripheral nerve using dental pulp cells

• Project/Area Number: 23792386
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Surgical dentistry
• Research Institution: Tokyo Women's Medical University
• Principal Investigator: SASAKI Ryo 東京女子医科大学, 医学部, 講師 (60524709)
• Project Period (FY): 2011 – 2012
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2012: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2011: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: 神経再生 / 歯髄 / 智歯 / 顔面神経 / 末梢神経再生 / 顔面神経再生

Research Abstract

Dental pulp is an easily obtainable cell-source of viable cells including Schwann and neural progenitor cells for peripheral nerve regeneration. A silicone tube containing rat dental pulp cells was transplanted into a gap of the facial nerve in rats. Tubulation with dental pulp cells could recover facial-nerve defect functionally and electrophysiologically in rats. Moreover, a silicone tube containing miniature pig dental pulp cells was transplanted into a gap of the facial nerve in miniature pigs. However, no nerve regeneration was observed 4 months after surgery. Further investigation was necessary for the peripheral nerve regeneration in miniature pigs.

Research Products

• [Journal Article] Electrophysiological and functional evaluations of regenerated facial-nerve defect with a tube containing dental pulp cells in rats (2014)
  • Author(s): Ryo Sasaki, Hajime Matsumine, Yorikatsu Watanabe, Yuichi Takeuchi, Masayuki Yamato, Teruo Okano, Mariko Miyata, Tomohiro Ando
  • Journal Title: Plastic and Reconstructive Surgery Volume: (in press)
• [Journal Article] Anesthesia for research on reconstructive facial surgery in rats (2013)
  • Author(s): Ryo Sasaki, Hajime Matsumine, Yorikatsu Watanabe, Masayuki Yamato, Tomohiro Ando
  • Journal Title: Journal of Reconstructive Microsurgery Volume: 29(03) Pages: 209-210
• [Journal Article] Surgical anatomy of the sural nerve for peripheral nerve reconstruction research in swine (2013)
  • Author(s): Ryo Sasaki, Hajime Matsumine, Yorikatsu Watanabe, Masayuki Yamato, Teruo Okano, Tomohiro Ando
  • Journal Title: Scandinavian Journal of Laboratory Animal Science Volume: (in press)
  • NAID: 120006602911
• [Journal Article] 歯髄細胞を組み込んだPLGA神経誘導管は顔面神経再生を促進する (2013)
  • Author(s): 佐々木亮, 松峯元, 渡辺頼勝
  • Journal Title: 日本口腔外科学会雑誌 Volume: 59(6) Pages: 404-411
  • NAID: 130004707962
• [Journal Article] Anesthesia for reconstructive surgical research in rat face (2013)
  • Author(s): Ryo Sasaki, Hajime Matsumine, Yorikatsu Watanabe, Masayuki Yamato, Tomohiro Ando
  • Journal Title: Journal of Reconstructive Microsurgery Volume: 29 Issue: 03 Pages: 209-2010
  • DOI: 10.1055/s-0032-1331147
  • Peer Reviewed
• [Journal Article] Spontaneous fibrosarcoma in an experimental aged Lewis rat (2012)
  • Author(s): Ryo Sasaki, Hajime Matsumine, Naoyuki Matsumoto, Yorikatsu Watanabe, Masayuki Yamato, Teruo Okano, Tomohiro Ando
  • Journal Title: Laboratory Animals Volume: 46 Pages: 352-355
• [Journal Article] Punch and spindle-shaped biopsies for collecting oral mucosal tissue for the fabrication of transplantable autologous epithelial cell sheets (2012)
  • Author(s): Ryo Sasaki, Masayuki Yamato, Ryo Takagi, Takashi Ohki, Hajime Matsumine, Teruo Okano, Tomohiro Ando
  • Journal Title: Journal of Biomedical Materials Research Part A Volume: 100 Issue: 10 Pages: 2849-2854
  • DOI: 10.1002/jbm.a.34216
  • Peer Reviewed
• [Journal Article] PLGA artificial nerve conduits with dental pulp cells promote facial nerve regeneration (2011)
  • Author(s): Ryo Sasaki, Shunsuke Aoki, Masayuki Yamato, Hiroto Uchiyama, Keiji Wada, Hideki Ogiuchi, Teruo Okano, Tomohiro Ando
  • Journal Title: Journal of Tissue Engineering and Regenerative Medicine Volume: 5 Pages: 823-830
  • NAID: 130004707962
• [Presentation] 歯髄細胞を組み込んだハイブリッド型神経誘導管によるラット顔面神経再生 : 機能的・電気生理学的検討 (2014)
  • Author(s): 佐々木亮, 安藤智博
  • Organizer: 第68回NPO法人日本口腔科学会学術集会
• [Presentation] 歯髄細胞を組み込んだハイブリッド型神経誘導管によるラット顔面神経再生：機能的・電気生理学的検討 (2014)
  • Author(s): 佐々木亮、安藤智博
  • Organizer: 第６８回NPO法人日本口腔科学会学術集会
  • Place of Presentation: 東京
• [Presentation] Anesthesia for research on facial reconstructive surgery in rats (2013)
  • Author(s): Ryo Sasaki, Hajime Matsumine, Yorikatsu Watanabe, Masayuki Yamato, Tomohiro Ando
  • Organizer: 21st International Conference on Oral and Maxillofacial Surgery, ICOMS2013
  • Place of Presentation: Barcelona, Spain
• [Presentation] Rat facial nerve defect model for facial nerve regeneration study (2013)
  • Author(s): Ryo Sasaki, Yorikatsu Watanabe, Hajime Matsumine, Masayuki Yamato, Sunsuke Aoki, Hiroto Uchiyama, Keiji Wada, Hideki Ogiuchi, Teruo Okano, Tomohiro Ando
  • Organizer: 12th International Facial Nerve Symposium
  • Place of Presentation: Liberty Hotel, Boston, MA
• [Presentation] Anesthesia for research on facial reconstructive surgery in rats (2013)
  • Author(s): Ryo Sasaki, Hajime Matsumine, Yorikatsu Watanabe, Masayuki Yamato, Tomohiro Ando
  • Organizer: 21st International Conference on Oral and Maxillofacial Surgery, ICOMS2013
  • Place of Presentation: バルセロナ、スペイン
• [Presentation] Rat facial nerve defect model for facial nerve regeneration study (2013)
  • Author(s): Ryo Sasaki, Yorikatsu Watanabe, Hajime Matsumine, Masayuki Yamato, Shunsuke Aoki, Hiroto Uchiyama, Keiji Wada, Hideki Ogiuchi, Teruo Okano and Tomohiro Ando
  • Organizer: 12th International Facial Nerve Symposium
  • Place of Presentation: ボストン、アメリカ
• [Presentation] 顔面神経再生研究におけるラット顔面神経欠損モデル (2012)
  • Author(s): 佐々木亮, 松峯元, 渡辺頼勝
  • Organizer: 第35回日本顔面神経研究会
  • Place of Presentation: 福島県猪苗代
• [Presentation] Biopsies for collecting oral mucosal tissue for tissue for tissue-engineered cell sheets (2012)
  • Author(s): Ryo Sasaki, Masayuki Yamato, Ryo Takagi, Takashi Ohki, Hajime Matsumine, Teruo Okano, Tomohiro Ando
  • Organizer: XXI Congress of the European Associstion for Cranio-Maxillo-Facial Surgery
  • Place of Presentation: クロアチア
• [Presentation] 顔面神経再生研究におけるラット顔面神経欠損モデル (2012)
  • Author(s): 佐々木亮 松峯元 渡辺頼勝
  • Organizer: 第35回日本顔面神経研究会
  • Place of Presentation: 福島
• [Presentation] SURGICAL ANATOMY OF THE SWINE FACE FOR MAXILLOFACIAL SURGERY (2011)
  • Author(s): Ryo Sasaki, Yorikatsu Watanabe, Hajime Matsumine, Masayuki Yamato, Teruo Okano, Tomohiro Ando
  • Organizer: 20th International Conference on Oral and Maxillofacial Surgery
  • Place of Presentation: Santiago, Chile
• [Presentation] ティッシュ・エンジニアリングのためのブタ顔面外科解剖 (2011)
  • Author(s): 佐々木亮, 渡辺頼勝, 安藤智博
  • Organizer: 第65回NPO法人日本口腔科学会学術集会
  • Place of Presentation: 東京都タワーホール船越
• [Presentation] 歯髄を細胞ソースとした神経再生 (2011)
  • Author(s): 佐々木亮, 青木俊介, 大和雅之, 渡辺頼勝, 松峯元, 内山博人, 扇内秀樹, 和田圭司, 岡野光夫, 安藤智博
  • Organizer: 第36回日本外科系連合学会学術集会
  • Place of Presentation: ヒルトン東京ベイ,千葉
• [Presentation] Surgical anatomy of the swine face for maxillofacial surgery (2011)
  • Author(s): Ryo Sasaki
  • Organizer: 20th International Conference on Oral and Maxillofacial Surgery
  • Place of Presentation: Santiago, Chile,
• [Presentation] 歯髄を細胞ソースとした神経再生
  • Author(s): 佐々木亮
  • Organizer: 第36回日本外科系連合学会学術集会(招待講演)
  • Place of Presentation: ヒルトン東京ベイ, 千葉