Human dental pulp stem cell sheet for tissue regeneration
| Project/Area Number |
23792392
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Surgical dentistry
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| Research Institution | Tsurumi University |
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Principal Investigator |
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| Project Period (FY) |
2011 – 2013
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| Project Status |
Completed (Fiscal Year 2013)
|
| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2013: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2012: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2011: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Keywords | 細胞治療 / 歯髄 / 細胞シート / 骨 / 軟骨 / 再生医療 / 骨再生 / 軟骨再生 / 神経再生 / 移植再生医療 / 再生医学 / 細胞・組織 |
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| Research Abstract |
The aim of this study was clinical application of the cell sheets fabricated from human Dental pulp stem cells (hDPSCs). we developed that hDPSCs spontaneously formed cell sheet structure through culturing for several weeks. The cell sheets expressed bone-related markers by immunofluorescence staining and reverse transcriptase-polymerase chain reaction (RT-PCR). In addition, these cell sheets were transplanted into bone defects of the skull in mice, and the bone formation was histologically observed. Next, chondrogenic cell sheets were fabricated by culturing hDPSCs in the chondrogenic induction medium, expressed cartilage-related markers by immunofluorescence staining and RT-PCR. Chondrogenic cell sheets implanted under the skin kept chondral characteristics without degradation at 6 weeks after implantation. The cell sheets fabricated from hDPSCs may be useful for bone or cartilage tissue engineering.
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Report
(4 results)
Research Products
(20 results)
