Peripheral nerve regeneration using an artificial nerve with dedifferentiated fat cells

• Project/Area Number: 23792397
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Surgical dentistry
• Research Institution: Osaka Dental University
• Principal Investigator: ANDO Kayoko 大阪歯科大学, 歯学部, 講師(非常勤) (10388366)
• Project Period (FY): 2011 – 2013
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000); Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2012: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2011: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
• Keywords: 脱分化脂肪細胞 / チタンファイバーメッシュ / 骨組織工学 / 天井培養 / 脂肪組織由来幹細胞 / 骨再生 / 組織工学 / 頬脂肪体 / 脂肪幹細胞 / 骨組織再生

Research Abstract

We isolated dedifferentiated fat (DFAT) cells using the ceiling culture method from rabbit subcutaneous fat. DFAT cells were seeded into titanium fiber mesh (TFM) and cultured in an osteogenic medium for 14 days. In scanning electron microscopic analysis, the number of DFAT cells increased, and a calcified matrix was observed around the fibers of TFM. The osteocalcin and calcium content in the TFM were significantly higher on day 14 compared to other time points. It was suggested that DFAT cells differentiated into osteoblasts in TFM. The combination of DFAT cells and TFM may be a useful option for bone tissue engineering.

Research Products

• [Journal Article] The osteoblastic differentiation ability of human dedifferentiated fat cells is higher than that of adipose stem cells from the buccal fat pad. (2014)
  • Author(s): Kishimoto N, Momota Y, Hashimoto Y, Tatsumi S, Ando K, Omasa T, Kotani J.
  • Journal Title: Clin Oral Investig. Volume: In press Issue: 8 Pages: 1893-1901
  • DOI: 10.1007/s00784-013-1166-1
  • Peer Reviewed
• [Journal Article] Dedifferentiated fat cells differentiate into osteoblasts in titanium fiber mesh (2013)
  • Author(s): Kishimoto N, Momota Y, Hashimoto Y, Ando K, Omasa T, Kotani J
  • Journal Title: Cytotechnology Volume: 65 Issue: 1 Pages: 15-22
  • DOI: 10.1007/s10616-012-9456-z
  • Peer Reviewed
• [Presentation] ヒト頬脂肪体由来脱分化脂肪細胞と脂肪幹細胞の細胞表面抗原解析 (2013)
  • Author(s): 岸本直隆, 百田義弘, 橋本典也, 安東佳代子, 大政健史, 小谷順一郎
  • Organizer: 第11回日本再生歯科医学会学術大会
  • Place of Presentation: 東京
  • Year and Date: 2013-08-31
• [Presentation] Comparison of Osteoblastic Differentiation Abilities in Dedifferentiated-Fat-Cells with Adipose-Stem-Cells (2013)
  • Author(s): 岸本直隆, 百田義弘, 橋本典也, 安東佳代子, 大政健史, 小谷順一郎
  • Organizer: The 91st International Association for Dental Research General Session & Exhibition
  • Place of Presentation: Seattle,Washington, USA
  • Year and Date: 2013-03-23
• [Presentation] 脱分化脂肪細胞を用いた顎骨再生におけるトランスレーショナル研究 (2013)
  • Author(s): 岸本直隆, 百田義弘, 橋本典也, 安東佳代子, 大政健史, 小谷順一郎
  • Organizer: 第29回「歯科医学を中心とした総合的な研究を推進する集い」
  • Place of Presentation: 東京
  • Year and Date: 2013-01-12
• [Presentation] Osteoblastic Differentiation Ability is higher in Human Dedifferentiated Fat Cells than in Adipose Stem Cells (2012)
  • Author(s): 岸本直隆, 百田義弘, 橋本典也, 安東佳代子, 大政健史, 小谷順一郎
  • Organizer: YABEC2012
  • Place of Presentation: 徳島
  • Year and Date: 2012-10-27
• [Presentation] ヒト脱分化脂肪細胞は脂肪幹細胞より骨芽細胞分化能が高い (2012)
  • Author(s): 岸本直隆, 百田義弘, 橋本典也, 安東佳代子, 坂本章人, 大政健史, 小谷順一郎
  • Organizer: 第10回日本再生歯科医学会学術大会
  • Place of Presentation: 神戸
  • Year and Date: 2012-09-02
• [Presentation] 脱分化脂肪細胞はTitanium Webスキャフォールド内で骨芽細胞へ分化する (2011)
  • Author(s): 岸本直隆，百田義弘，橋本典也，安東佳代子，坂本章人，大政健史，小谷順一郎．
  • Organizer: 第9回日本再生歯科医学会学術大会
  • Place of Presentation: 大阪府 大阪国際会議場
• [Presentation] ヒト頬脂肪体由来脱分化脂肪細胞と脂肪幹細胞の細胞表面抗原解析
  • Author(s): 岸本直隆、百田義弘、橋本典也、安東佳代子、大政健史、小谷順一郎
  • Organizer: 第11回日本再生歯科医学会学術大会
  • Place of Presentation: 日本大学理学部駿河台キャンパスCSTホール
• [Presentation] ヒト脱分化脂肪細胞は脂肪幹細胞より骨芽細胞分化能が高い
  • Author(s): 岸本 直隆、百田 義弘、橋本 典也、安東 佳代子、坂本 章人、大政 健史、小谷 順一郎
  • Organizer: 第10回日本再生歯科医学会学術大会・総会
  • Place of Presentation: 神戸