Analysis of osteoclastogenesis regulated by orthodontic force

• Project/Area Number: 23792455
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Orthodontic/Pediatric dentistry
• Research Institution: Matsumoto Dental University
• Principal Investigator: ARAI Atsushi 松本歯科大学, 歯学部, 助教 (00532772)
• Project Period (FY): 2011 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000); Fiscal Year 2012: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2011: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
• Keywords: 破骨細胞 / 破骨細胞前駆細胞 / RANK / RANKL / c-Fos / M-CSF / c-fmsマクロファージ / 矯正力 / 歯の移動 / c-fms

Research Abstract

We previously reported that osteoclast precursors were detected as RANK-positive cells in bone tissues. RANK-positive cells were observed in bone tissues in RANKL-/- mice, but not in c-Fos-/- mice. c-Fos, but not RANKL, is required for the up-regulation of RANK in osteoclast precursors. Then, we analyzed the mechanism of c-Fos-mediated up-regulation of RANK in osteoclast precursors. Our results suggest that c-Fos plays essential roles not only in RANKL-induced formation of osteoclasts but also in M-CSF-induced formation of osteoclast precursors.

Research Products

• [Journal Article] Spleen serves as a reservoir of osteoclast precursors through vitamin D-induced IL-34 expression in osteopetrotic op/op mice. (2012)
  • Author(s): Nakamichi Y et al.
  • Journal Title: Proc Natl Acad Sci USA Volume: 109 Issue: 25 Pages: 10006-10011
  • DOI: 10.1073/pnas.1207361109
  • Peer Reviewed
• [Journal Article] c-Fos plays an essential role in the up-regulation of RANK expression in osteoclast precursors within the bone microenvironment (2012)
  • Author(s): Arai A, et al
  • Journal Title: J Cell Sci Volume: (印刷中) Pages: 2910-2917
  • DOI: 10.1242/jcs.099986
  • Peer Reviewed
• [Journal Article] Lineage-Committed Osteoclast Precursors Circulate in Blood and Settle Down Into Bone (2011)
  • Author(s): Muto, A, Mizoguchi, T, Udagawa, N., Ito, S, Kawahara, I, Abiko, Y, Arai, A., Harada, S., Kobayashi, Y., Nakamichi, Y, Penninger, JM, Noguchi, T, Takahashi, N.
  • Journal Title: Journal of Bone and Mineral Research Volume: 26 Issue: 12 Pages: 2978-2990
  • DOI: 10.1002/jbmr.490
  • Peer Reviewed
• [Presentation] 2012 the American Society for Bone and Mineral Research Annual Meeting 2012 年 10月 c-Fos plays an essential role in up-regulation of RANK expression in osteoclast precursors A. Arai (2012)
  • Author(s): T. Mizoguchi , Y. Kobayashi ,T. Yamashita,K. Yamada , J. M. Penninger , N. Udagawa,N. Takahashi
  • Organizer: Journal of Bone and Mineral Research 23, suppl 1: S179,
• [Presentation] c-Fos plays an essential role in up-regulation of RANK expression in osteoclast precursors (2012)
  • Author(s): Atsushi Arai
  • Organizer: 2012 the American Society for Bone and Mineral Research Annual Meeting
  • Place of Presentation: Minneapolis USA
• [Presentation] IOF Regionals 2nd Asia-Pacific Osteoporosis and Bone Meeting ANZBMS Annual Scientific Meeting, with JSBMR2011年 9月BMP-induced ectopic bone formation in c-Fos-deficient mice (2011)
  • Author(s): M. Nakamura, T. Ninomiya, T. Mizoguchi, A. Arai, N. Takahashi, N. Udagawa
  • Organizer: 第2回アジア太平洋骨粗鬆症学会プログラム抄録集
• [Presentation] IOF Regionals 2nd Asia-Pacific Osteoporosis and Bone Meeting ANZBMS Annual Scientific Meeting, with JSBMR2011 年 9 月 c-Fos plays an essential role in up-regulation of RANK expression in osteoclast precursors (2011)
  • Author(s): A. Arai, T. Mizoguchi , Y. Kobayashi ,T.Yamashita, K. Yamada , J. M. Penninger , N. Udagawa, N. Takahashi
  • Organizer: 第2回アジア太平洋骨粗 鬆症学会プログラム抄録集
• [Presentation] in vivoにおける破骨細胞分化機構の解析-M-CSFはc-Fosを介し前駆細胞のRANKを上昇する (2011)
  • Author(s): 荒井敦, 溝口利英, 小林泰浩, 山 下照仁, 山田一尋, 宇田川信之, 高橋直之
  • Organizer: 松本歯科大学学会
• [Presentation] 生体内における破骨細胞分化機構の解析-M-CSF は c-Fos を介して破骨細胞前駆細胞の RANK 発現を上昇する- (2011)
  • Author(s): 荒井 敦 , 溝口利英, 原田卓, 武藤昭紀, 小林泰浩, 山 下照仁, 保田尚孝, 山田一尋,宇田川信 之, 高橋直之
  • Organizer: 第29回日本骨代謝学会
• [Presentation] 月c-Fos 遺伝子欠損マウスを用いた BMP 誘導 性異所性骨形成に関する解析中村みどり (2011)
  • Author(s): 二宮 禎,溝口利英,荒 井敦 , 高橋直之, 宇田川信之
  • Organizer: 第29回日本骨代謝学会
• [Presentation] 生体内における破骨細胞分化機構の解析-M-CSFはc-Fosを介して破骨細胞前駆細胞のRANK発現を上昇する (2011)
  • Author(s): 荒井敦, 溝口利英, 原田卓, 武藤昭紀, 小林泰浩, 山下照仁, 保田尚孝, 山田一尋,宇田川信之,高橋直之
  • Organizer: 第29回日本骨代謝学会学術集会
  • Place of Presentation: 大阪国際会議場（大阪市）
• [Presentation] c-Fos plays an essential role in up-regulation of RANK expression in osteoclast precursors (2011)
  • Author(s): Atsushi Arai, Toshihide Mizoguchi, Yasuhiro Kobayashi,Teruhito Yamashita, Kazuhiro Yamada, Josef M. PenningerNobuyuki Udagawa , and Naoyuki Takahashi
  • Organizer: IOF Regionals Asia-Pacific Osteoporosis and Bone Meeting
  • Place of Presentation: Australia(Gold Coast)