Effect of periodontitis and smoking for genome methylation on IL-6 gene promoter region

• Project/Area Number: 23792473
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Periodontal dentistry
• Research Institution: Niigata University
• Principal Investigator: MOROZUMI Toshiya 新潟大学, 医歯学総合病院, 助教 (20444151)
• Project Period (FY): 2011 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2012: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000); Fiscal Year 2011: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: IL-6 / DNA メチル化 / 喫煙 / 歯周炎 / 遺伝子解析

Research Abstract

Conventional gene polymorphism analysis cannot elucidate the function and activity of the disease sensitivity gene. Also, it is known that discordant exist between polymorphism and protein production in some genes. The possible cause is that the expression of gene information is influenced by DNA methylation modification. Thus, analysis of genome methylation which regulates the gene activity is necessary for research of the disease sensitivity gene. In the study, DNA extracted from blood, gingival crevicular fluid and periodontal tissues samples in the four type subjects (Healthy individuals, periodontitis patients, smoker with/without periodontitis) were used to investigate the effect of periodontitis and smoking for genome methylation on the IL-6 gene promoter region.

Research Products

• [Journal Article] Gene and protein localisation of Tumor Necrosis Factor (TNF) -α converting enzyme in gingival tissues from periodontitis patients with drug-induced gingival overgrowth (2013)
  • Author(s): Tomita T, Kubota T, Nakasone N, Morozumi T, Abe D, Maruyama S, Shimizu T, Horimizu M, Saku T, Yoshie H
  • Journal Title: Archives of Oral Biology Volume: (in press) Issue: 8 Pages: 1014-20
  • DOI: 10.1016/j.archoralbio.2013.02.011
  • Peer Reviewed
• [Journal Article] Over20特集危険!そのSRPが菌血症を招いている (2013)
  • Author(s): 両角俊哉、吉江弘正
  • Journal Title: 歯科衛生士2013年3月号 Volume: 37巻3号 Pages: 16-26
• [Journal Article] Strategy for Preventing Periodontal Disease according to Various Life Stages (2013)
  • Author(s): 森田学、稲垣幸司、王宝禮、埴岡隆、藤井健男、両角俊哉、伊藤弘、山本龍生、吉江弘正
  • Journal Title: Nihon Shishubyo Gakkai Kaishi (Journal of the Japanese Society of Periodontology) Volume: 54 Issue: 4 Pages: 352-374
  • DOI: 10.2329/perio.54.352
  • NAID: 10031145212
  • ISSN: 0385-0110, 1880-408X
  • Peer Reviewed
• [Journal Article] Effects of Erbium-Doped: Yttrium Aluminum Garnet (Er: YAG) laser on bacteremia due to scaling and root planing (2012)
  • Author(s): Komatsu Y, Morozumi T, Abe D, Okada M, Nakasone N, Okuda K, Yoshie H
  • Journal Title: Journal of Lasers in Medical Sciences Volume: 3(4) Pages: 175-84
  • URL: http://www.journals.sbmu.ac.ir/jlms/article/view/3353
  • Peer Reviewed
• [Journal Article] Effects of Erbium-Doped: Yttrium Aluminum Garnet (Er: YAG) laser on bacteremia due to scaling and root planing (2012)
  • Author(s): Yasutaka Komatsu, Toshiya Morozumi et al.
  • Journal Title: Journal of Lasers in Medical Sciences Volume: 3 Pages: 175-184
  • Peer Reviewed
• [Journal Article] ポジションペーパー（学会見解論文） 生涯を通じての歯周病対策 －セルフケア, プロフェッショナルケア, コミュニティーケア－ (2012)
  • Author(s): 森田 学、両角 俊哉ら
  • Journal Title: 日本歯周病学会会誌 Volume: 54 Pages: 352-374
  • Peer Reviewed
• [Journal Article] ヘルスケアプロ バイダーとしての歯周病感染リスクマネジ メント (2011)
  • Author(s): 両角俊哉、吉江弘正
  • Journal Title: 抗菌療法、菌血症対策と歯周病感染リスクマネジメント Volume: 5巻7号 Pages: 36-39
• [Journal Article] 乳酸菌配合シュガーレスガムが歯周病患者唾液中の歯周病原細菌叢へ与える影響 (2011)
  • Author(s): 山口人巳、久保田健彦、濃野要、両角俊哉、飯山真奈美、川崎健司、吉江弘正
  • Journal Title: 日本歯科保存学雑誌 Volume: 第54巻第6号 Pages: 466-474
  • NAID: 110009419175
  • URL: http://www.hozon.or.jp/member/kaisi_vol54_no6.html
  • Peer Reviewed
• [Journal Article] Altered gene expression in leukocyte transendothelial migration and cell communication pathways in periodontitis-affected gingival tissues (2011)
  • Author(s): Abe D, Kubota T, Morozumi T, Shimizu T, Nakasone N, Itagaki M, Yoshie H
  • Journal Title: J Periodontal Res Volume: 46 Issue: 3 Pages: 345-353
  • DOI: 10.1111/j.1600-0765.2011.01349.x
  • Peer Reviewed
• [Journal Article] Position PaperSmoking and periodontal tissue (2011)
  • Author(s): 大森みさき、両角俊哉、稲垣幸司、横田誠、沼部幸博、佐藤聡、伊藤弘、王宝禮、上田雅俊、山田了、伊藤公一
  • Journal Title: Nihon Shishubyo Gakkai Kaishi (Journal of the Japanese Society of Periodontology) Volume: 53 Issue: 1 Pages: 40-49
  • DOI: 10.2329/perio.53.40
  • NAID: 10028051358
  • ISSN: 0385-0110, 1880-408X
  • Peer Reviewed
• [Journal Article] Micro array and quantitative RT-PCR analyses in calcium-channel blockers induced gingival overgrowth tissues of periodontitis patients (2011)
  • Author(s): Shimizu T, Kubota T, Nakasone N, Abe D, Morozumi T, Yoshie H
  • Journal Title: Arch Oral Biol Volume: 56 Issue: 3 Pages: 277-284
  • DOI: 10.1016/j.archoralbio.2010.10.002
  • Peer Reviewed
• [Journal Article] 喫煙の歯周組織に対する影響 (2011)
  • Author(s): 大森みさき、両角俊哉、稲垣幸司、横田誠、沼部幸博、佐藤聡、伊藤弘、王宝禮、上田雅俊、山田了、伊藤公一
  • Journal Title: 日本歯周病学会会誌 Volume: 53 Pages: 40-49
  • NAID: 10028051358
  • Peer Reviewed
• [Journal Article] 乳酸菌配合シュガーレスガムが歯周病患者唾液中の歯周病原細菌叢へ与える影響 (2011)
  • Author(s): 山口人巳、久保田健彦、濃野 要、両角俊哉、飯山真奈美、川崎健司、吉江弘正
  • Journal Title: 日本歯科保存学雑誌 Volume: 54 Pages: 466-474
  • NAID: 110009419175
  • Peer Reviewed
• [Presentation] Platlet-richfibrin(PRF)によるヒト培養骨膜シートの機能向上 (2013)
  • Author(s): 堀水慎、川瀬知之、久保田健彦、永田昌毅、奥田一博、冨田尊志、両角俊哉、吉江弘正
  • Organizer: 第12回日本再生医療学会総会
  • Place of Presentation: 横浜市
  • Year and Date: 2013-03-22
• [Presentation] TNF-aconvertingenzyme(TACE,ADAM-17) (2012)
  • Author(s): 冨田尊志、久保田健彦、中曽根直弘、両角俊哉、堀水慎、吉江弘正
  • Organizer: 平成24年度新潟歯学会第2回例会
  • Place of Presentation: 新潟市
  • Year and Date: 2012-11-10
• [Presentation] 細菌検査・抗体価検査によるSPT期再発の予知判定 (2012)
  • Author(s): 両角俊哉
  • Organizer: 第55回秋季日本歯周病学会学術大会シンポジウム1歯周炎の再発リスクは予測できるか
  • Place of Presentation: つくば国際会議場、つくば市
  • Year and Date: 2012-09-23
• [Presentation] 口腔内歯周病原細菌および口臭に対するBFR(ブラッシング+フロッシング+リンシング)の効果 (2012)
  • Author(s): 藤岡陽介、両角俊哉、久保田健彦、吉江弘正
  • Organizer: 平成24年度新潟歯学会第1回例会
  • Place of Presentation: 新潟市
  • Year and Date: 2012-07-14
• [Presentation] 歯科における禁煙支援の実際 (2012)
  • Author(s): 両角俊哉
  • Organizer: 平成23年度社団法人新潟県歯科衛生士会第4回研修会
  • Place of Presentation: 新潟県歯科医師会館、新潟市
  • Year and Date: 2012-03-25
• [Presentation] SPT期における細菌および抗体価検査値の変動 (2012)
  • Author(s): 両角俊哉、中川種昭、川浪雅光、高橋慶壮、佐藤聡、齋藤淳、小方頼昌、三辺正人、和泉雄一、沼部幸博、伊藤公一、吉成伸夫、野口俊英、梅田誠、前田勝正、原宜興、野口和行、高柴正悟、野村義明、吉江弘正
  • Organizer: 第22回日本歯科医学会総会
  • Place of Presentation: 大阪市
• [Presentation] Effect of BFR for periodontopathic bacteria in oral cavity and halitosis (2012)
  • Author(s): Fujioka Y, Morozumi T, Kubota T, Yoshie H
  • Organizer: The98th Annual Meeting American Academy of Periodontology in collaboration with the Japanese Society of Periodontology (Research Forum Poster Session)
  • Place of Presentation: Los Angeles, U.S.A
• [Presentation] Bacterial counts and serum antibody levels against periodontal bacteria during SPT phase (2012)
  • Author(s): Morozumi T, Nakagawa T, Kawanami M, Takahashi K, Sato S, Saito A, Ogata Y, Minabe M, Izumi Y, Numabe Y, Ito K, Yoshinari N, Noguchi T, Umeda M, Maeda K, Hara Y, Noguchi K, Takashiba S, Kakuta E, Nomura Y, Hanada N, Yoshie H
  • Organizer: The98th Annual Meeting American Academy of Periodontology in collaboration with the Japanese Society of Periodontology (Research Forum Poster Session)
  • Place of Presentation: Los Angeles, U.S.A
• [Presentation] Amyloid beta (A4) precursor protein expression in periodontitis-affected gingival tissues (2012)
  • Author(s): Kubota T, Mauyama S, Abe D, Shimizu T, Nakasone N, Morozumi T, Saku T, Yoshie H
  • Organizer: The 98th Annual Meeting American Academy of Periodontology incollaboration with the Japanese Society of Periodont
  • Place of Presentation: Los Angeles, U.S.A
• [Presentation] Attitude to tobacco control policy and social nicotine dependence among Japanese periodontitis (2012)
  • Author(s): Inagaki K, Ohmori M, Morozumi T, Numabe Y, Sato S, Noguchi T
  • Organizer: The 98th Annual Meeting American Academy of Periodontology in collaboration with the Japanese Society of Periodontology (Research Forum Poster Session)
  • Place of Presentation: Los Angeles, U.S.A
• [Presentation] Molecular biological factors in drug-induced gingival overgrowth (2012)
  • Author(s): Shimizu T, Kubota T, Iwasaki M, Morozumi T, Nakasone N, Yoshie H
  • Organizer: The 98th Annual Meeting American Academy of Periodontology in collaboration with the Japanese Society of Periodontology (Research Forum Poster Session)
  • Place of Presentation: Los Angeles, U.S.A
• [Presentation] The combinational use of periosteal sheet and platelet-rich fibrin (2012)
  • Author(s): Horimizu M, Kawase T, Kubota T, Nagata M, Okuda K, Timita T, Morozumi T, Yoshie H
  • Organizer: The 98th Annual Meeting American Academy of Periodontology in collaboration with the Japanese Society of Periodontology (Research Forum Poster Session)
  • Place of Presentation: Los Angeles, U.S.A
• [Presentation] 歯周炎罹患部位歯肉組織におけるToll-likereceptorsignalingpathway関連遺伝子の上昇 (2012)
  • Author(s): 阿部大輔、久保田健彦、両角俊哉、中曽根直弘、清水太郎、吉江弘正
  • Organizer: 日本歯科保存学会2012年度春季学術大会(第136回)
  • Place of Presentation: プログラム、宜野湾市
• [Presentation] Platelet-richfibrinとの複合化によるヒト培養骨膜シートの機能向上 (2012)
  • Author(s): 堀水慎、久保田健彦、奥田一博、冨田尊志、両角俊哉、吉江弘正、川瀬知之
  • Organizer: 日本歯科保存学会2012年度春季学術大会(第136回)
  • Place of Presentation: プログラム、宜野湾市
• [Presentation] 口腔内歯周病原細菌および病的口臭に対するBFR(ブラッシング+フロッシング+ リンシング)の効果 (2012)
  • Author(s): 藤岡陽介、両角俊哉、久保田健彦、吉江弘正
  • Organizer: 日本歯科保存学会2012年度春季学術大会(第136回)
  • Place of Presentation: プログラム、宜野湾市
• [Presentation] Gene and protein expression of TNF-α converting enzyme (TACE/ADAM17) and tissue inhibitor of metalloproteinases (TIMP)-3 in gingival tissues from periodontitis patients with drug-induced gingival overgrowth (2012)
  • Author(s): Tomita T, Kubota T, Nakasone N, Iiyama M, Morozumi T, Horimizu M, Abe D, Nagata K, Nohno H, Yoshie H
  • Organizer: EUROPERIO7 (Poster presentations)
  • Place of Presentation: Vienna, Austria
• [Presentation] Amyloid beta (A4) precursor protein expression in periodontitis-affected gingival tissues (2012)
  • Author(s): Kubota T, Maruyama D, Abe D, Shimizu T, Nakasone N, Morozumi T, Saku T, Yoshie H
  • Organizer: EUROPERIO7 (Poster presentations)
  • Place of Presentation: Vienna, Austria
• [Presentation] Toll-like receptor (TLR) signaling pathway is upregulated in periodontitis-affected gingival tissues (2012)
  • Author(s): Abe D, Kubota T, Morozumi T, Nakasone N, Shimizu T, Yoshie H
  • Organizer: EUROPERIO7 (Poster presentations)
  • Place of Presentation: Vienna, Austria
• [Presentation] Toll-like receptor (TLR) signaling pathway is upregulated in periodontitis-affected gingival tissues (2012)
  • Author(s): Daisuke Abe, Toshiya Morozumi et al.
  • Organizer: EUROPERIO7
  • Place of Presentation: Messe Wien Exhibition & Conference Center (Vienna, Austria)
• [Presentation] Amyloid beta (A4) precursor protein expression in periodontitis-affected gingival tissues (2012)
  • Author(s): Takehiko Kubota, Toshiya Morozumi et al.
  • Organizer: EUROPERIO7
  • Place of Presentation: Messe Wien Exhibition & Conference Center (Vienna, Austria)
• [Presentation] Gene and protein expression of TNF-α converting enzyme (TACE/ADAM17) and tissue inhibitor of metalloproteinases (TIMP)-3 in gingival tissues from periodontitis patients with drug-induced gingival overgrowth (2012)
  • Author(s): Takayuki Tomita, Toshiya Morozumi et al.
  • Organizer: EUROPERIO7
  • Place of Presentation: Messe Wien Exhibition & Conference Center (Vienna, Austria)
• [Presentation] 口腔内歯周病原細菌および病的口臭に対するBFR(ブラッシング＋フロッシング＋リンシング)の効果 (2012)
  • Author(s): 藤岡 陽介、両角 俊哉ら
  • Organizer: 日本歯科保存学会2012年度春季学術大会（第136回）
  • Place of Presentation: 沖縄コンベンションセンター（沖縄県）
• [Presentation] Platelet-rich fibrinとの複合化によるヒト培養骨膜シートの機能向上 (2012)
  • Author(s): 堀水 慎、両角 俊哉ら
  • Organizer: 日本歯科保存学会2012年度春季学術大会（第136回）
  • Place of Presentation: 沖縄コンベンションセンター（沖縄県）
• [Presentation] 歯周炎罹患部位歯肉組織におけるToll-like receptor signaling pathway関連遺伝子の上昇 (2012)
  • Author(s): 阿部 大輔、両角 俊哉ら
  • Organizer: 日本歯科保存学会2012年度春季学術大会（第136回）
  • Place of Presentation: 沖縄コンベンションセンター（沖縄県）
• [Presentation] 口腔内歯周病原細菌および口臭に対するBFR (ブラッシング＋フロッシング＋リンシング)の効果 (2012)
  • Author(s): 藤岡 陽介、両角 俊哉ら
  • Organizer: 平成24年度新潟歯学会第１回例会
  • Place of Presentation: 新潟大学歯学部（新潟県）
• [Presentation] The combinational use of periosteal sheet and platelet-rich fibrin (2012)
  • Author(s): Makoto Horimizu, Toshiya Morozumi et al.
  • Organizer: The 98th Annual Meeting American Academy of Periodontology in collaboration with the Japanese Society of Periodontology
  • Place of Presentation: Los Angeles Convention Center (Los Angeles, U.S.A.)
• [Presentation] Molecular biological factors in drug-induced gingival overgrowth (2012)
  • Author(s): Taro Shimizu, Toshiya Morozumi et al.
  • Organizer: The 98th Annual Meeting American Academy of Periodontology in collaboration with the Japanese Society of Periodontology
  • Place of Presentation: Los Angeles Convention Center (Los Angeles, U.S.A.)
• [Presentation] Attitude to tobacco control policy and social nicotine dependence among Japanese periodontitis (2012)
  • Author(s): Kouji Inagaki, Toshiya Morozumi et al.
  • Organizer: The 98th Annual Meeting American Academy of Periodontology in collaboration with the Japanese Society of Periodontology
  • Place of Presentation: Los Angeles Convention Center (Los Angeles, U.S.A.)
• [Presentation] Amyloid beta (A4) precursor protein expression in periodontitis-affected gingival tissues (2012)
  • Author(s): Takehiko Kubota, Toshiya Morozumi et al.
  • Organizer: The 98th Annual Meeting American Academy of Periodontology in collaboration with the Japanese Society of Periodontology
  • Place of Presentation: Los Angeles Convention Center (Los Angeles, U.S.A.)
• [Presentation] Bacterial counts and serum antibody levels against periodontal bacteria during SPT phase (2012)
  • Author(s): Toshiya Morozumi et al.
  • Organizer: The 98th Annual Meeting American Academy of Periodontology in collaboration with the Japanese Society of Periodontology
  • Place of Presentation: Los Angeles Convention Center (Los Angeles, U.S.A.)
• [Presentation] Effect of BFR for periodontopathic bacteria in oral cavity and halitosis (2012)
  • Author(s): Yousuke Fujioka, Toshiya Morozumi et al.
  • Organizer: The 98th Annual Meeting American Academy of Periodontology in collaboration with the Japanese Society of Periodontology
  • Place of Presentation: Los Angeles Convention Center (Los Angeles, U.S.A.)
• [Presentation] 細菌検査・抗体価検査によるSPT期再発の予知判定（シンポジウム１ 歯周炎の再発リスクは予測できるか） (2012)
  • Author(s): 両角 俊哉ら
  • Organizer: 第55回秋季日本歯周病学会学術大会
  • Place of Presentation: つくば国際会議場（茨城県）
  • Invited
• [Presentation] SPT期における細菌および抗体価検査値の変動 (2012)
  • Author(s): 両角 俊哉ら
  • Organizer: 第22回日本歯科医学会総会
  • Place of Presentation: インテックス大阪（大阪府）
• [Presentation] TNF-a converting enzyme (TACE, ADAM-17), TIMP-3の歯周組織発現解析および歯肉増殖症への関与 (2012)
  • Author(s): 冨田 尊志、両角 俊哉ら
  • Organizer: 平成24年度新潟歯学会第2回例会
  • Place of Presentation: 新潟大学歯学部（新潟県）
• [Presentation] TACE及びTIMP-3の薬物性歯肉増殖症と歯周炎の歯肉組織における遺伝子発現とタンパク質局在 (2011)
  • Author(s): 冨田尊志、久保田健彦、中曽根直弘、飯山真奈美、両角俊哉、永田昌毅、堀水慎、濃野要、吉江弘正
  • Organizer: 第54回秋季日本歯周病学会学術大会
  • Place of Presentation: 下関市
  • Year and Date: 2011-09-24
• [Presentation] Er:YAGレーザーによるSRP治癒効果および菌血症予防効果の検討 (2011)
  • Author(s): 小松康高、両角俊哉、阿部大輔、岡田萌、奥田一博、中曽根直弘、吉江弘正、小林哲夫
  • Organizer: 日本歯科保存学会2011年度秋季学術大会(第135回)、プログラム
  • Place of Presentation: 大阪市
• [Presentation] Er:YAGレーザーによるSRP治癒効果および菌血症予防効果の検討 (2011)
  • Author(s): 小松康高、両角俊哉、阿部大輔、岡田萌、奥田一博、中曽根直弘、吉江弘正、小林哲夫
  • Organizer: 日本歯科保存学会2011年度秋季学術大会（第135回）
  • Place of Presentation: 大阪市
• [Book] 「歯科衛生士」 Over 20特集 危険！そのSRPが菌血症を招いている (2013)
  • Author(s): 両角 俊哉、吉江 弘正
  • Total Pages: 11
  • Publisher: クインテッセンス出版