Identification of novel amelogenin-binding proteins by proteomics analysis
Project/Area Number |
23792480
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Research Category |
Grant-in-Aid for Young Scientists (B)
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Allocation Type | Multi-year Fund |
Research Field |
Periodontal dentistry
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Research Institution | Kyushu University |
Principal Investigator |
FUKUDA Takao 九州大学, 大学病院, その他 (80507781)
|
Project Period (FY) |
2011 – 2013
|
Project Status |
Completed (Fiscal Year 2013)
|
Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2013: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2012: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2011: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
|
Keywords | アメロジェニン / 歯周組織 / 再生医学 / 歯学 / プロテーム / 再生 / プロテオーム |
Research Abstract |
Amelogenin, the major component of enamel matrix derivative (Straumann® Emdogain), is a suggested bioactive candidate for promoting the regeneration of bone and periodontal tissues. In this study, we combined affinity chromatography and proteomic analysis to characterize amelogenin-binding proteins in an osteoblast cell line. Among the identified amelogenin-interacting proteins, we validated the biological interaction of amelogenin with Grp78, which was identified in both cytosolic and membrane-enriched fractions. We demonstrated that the interaction of amelogenin with Grp78 contributed to cell proliferation, rather than correlate with the osteogenesis in SaOS-2 cells. These findings suggest that the differential effects of amelogenin-derived osteoblast activation could be of potential clinical significance for understanding the cellular and molecular bases of amelogenin-induced periodontal tissue regeneration.
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Report
(4 results)
Research Products
(40 results)
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[Presentation] The effects of sprouty2 in periodontal ligament cell2013
Author(s)
Tanaka Urara, Toyoda Kyosuke, Sanui Terukazu, Fukuda Takao, Takaharu Taketomi, Atomura Ryo, Hamachi Takafumi, Maeda Katsumasa
Organizer
91nd General Session & Exhibition of the IADR
Place of Presentation
Washington State Convention Center in Seattle, Washington, USA
Related Report
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[Presentation] Proteome analysis of amelogenin-binding proteins2012
Author(s)
Takao Fukuda, Kyousuke Toyoda, Terukazu Sanui, Urara Tanaka, Takaharu Taketomi, Ryou Atomura, Takafumi Hamachi, Katsumasa Maeda
Organizer
The 6th International Assosiation for Dental Research/Pan European Region Congress and Exhibition
Place of Presentation
Finlandia Hall, Helsinki, Finland
Related Report
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[Presentation] Periodontal regenerative effect by inhibition of Sprouty22012
Author(s)
Terukazu Sanui, Urara Tanaka, Takao Fukuda, Kyousuke Toyoda, Takaharu Taketomi, Ryou Atomura, Takafumi Hamachi and Katsumasa Maeda
Organizer
The 6th International Assosiation for Dental Research/Pan European Region Congress and Exhibition
Place of Presentation
Finlandia Hall, Helsinki, Finland
Related Report
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