| Project/Area Number |
23792483
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Periodontal dentistry
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| Research Institution | Kagoshima University |
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Principal Investigator |
HASEGAWA KOZUE 鹿児島大学, 医歯(薬)学総合研究科, 助教 (00404492)
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| Co-Investigator(Renkei-kenkyūsha) |
NOGUCHI Kazuyuki 鹿児島大学, 医歯学総合研究科, 教授 (90218298)
DOUCHI Tsutomu 鹿児島大学, 医歯学総合研究科, 教授 (60150413)
NAKAMURA Toshiaki 鹿児島大学, 医歯学総合研究科, 助教 (60381183)
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| Research Collaborator |
HATAE Masayuki 医療法人聖成会, 柿木病院, 副院長
KAWAMATA Kazuya 愛育病院, 副院長
TATEISHI Fumi 医療法人社団忠重会 忠重歯科医院, 歯科医師
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| Project Period (FY) |
2011-04-28 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2012: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
Fiscal Year 2011: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Keywords | 歯周病 / 早産・低体重児出産 / 歯周病原細菌 / Porpyoromonas gingivalis / 早産 / 低体重児出産 / 子宮内感染 |
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| Outline of Final Research Achievements |
F. nucleatum was detected in all oral samples and seven chorionic tissues of the high-risk pregnant women, but was not detected in chorionic tissues of the normal pregnant women. F. nucleatum LPS significantly increased IL-6 and CRH secretion by chorion-derived cells. The F. nucleatum LPS-induced IL-6 and CRH levels were significantly reduced in TLR-2 or TLR-4 gene-silenced chorion-derived cells.We suggest that F. nucleatum is detected in chorionic tissues of high-risk pregnant women, but not in chorionic tissues of normal pregnant women, and that F. nucleatum induces IL-6 and CRH production via both TLR-2 and TLR-4 in chorion-derived cells.In vivo study, P.gingivalis was detected in fetalmembrane and placenta from mice of which P.gingivalis was injected into the teil vein. The TLR-2 and TLR-4 mRNA expression were significantly increased in fetal membrane and placenta from P. gingivalis injected mice compared with those from non-infected mice.
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