Expression and function in bone formation of extracellular matrix protein POEM

• Project/Area Number: 23792488
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Periodontal dentistry
• Research Institution: Showa University
• Principal Investigator: MIYAZONO Agasa 昭和大学, 歯学部, 助教 (00514936)
• Co-Investigator(Renkei-kenkyūsha): KAMIJO Ryutaro 昭和大学, 歯学部, 教授 (70233939) ; YAMADA Atsushi 昭和大学, 歯学部, 講師 (50407558)
• Project Period (FY): 2011 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2012: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2011: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: 歯周免疫機能学 / 骨芽細胞 / 細胞外マトリックス / TNFα

Research Abstract

POEM(pre-osteoblast epidermal growth factor-like repeat protein with meprin, A5 protein, and receptor protein-tyrosine phosphatase u domain) gene expression was suppressed by TNF-a via NF-kB. Previously, we reported that TGF-ss also suppressed POEM gene expression via ERK1/2 and JNK. Furthermore, we showed that POEM expression suppressed by TGF-ss via smad2.

Research Products

• [Journal Article] Nephronectin expression is regulated by SMAD signaling in osteoblast-like MC3T3-E1 cells (2012)
  • Author(s): Tsukasaki M, Yamada A, Yoshimura K,Miyazono A, Yamamoto M, Takami M, Miyamoto Y, Morimura N, Kamijo R
  • Journal Title: Biochem Biophys Res Commun Volume: 425 Pages: 390-392
  • Peer Reviewed
• [Journal Article] Nephronectin expression is regulated by SMAD signaling in osteoblast-like MC3T3-E1 cells. (2012)
  • Author(s): Tsukasaki M, Yamada A, Yoshimura K, Miyazono A, Yamamoto M, Takami M, Miyamoto Y, Morimura N, Kamijo R
  • Journal Title: Biochem Biophys Res Commun Volume: 425 Pages: 390-392
• [Journal Article] Expression of POEM, a positive regulator of osteoblast differentiation, is suppressed by TNF-a (2011)
  • Author(s): Tsukasaki M, Yamada A, Suzuki D, Aizawa R, Miyazono A, Miyamoto Y, Suzawa T, Takami M, Yoshimura K, Morimura N, Yamamoto M, Kamijo R
  • Journal Title: Biochem Biophys Res Commun Volume: 410 Pages: 766-770
  • Peer Reviewed
• [Journal Article] Expression of POEM, a positive regulator of osteoblast differentiation, is suppressed by TNF-α. (2011)
  • Author(s): Tsukasaki M, Yamada A, Suzuki D, Aizawa R, Miyazono A, Miyamoto Y, Suzawa T, Takami M, Yoshimura K, Morimura N, Yamamoto M, Kamijo R.
  • Journal Title: Biochem Biophys Res Commun Volume: 410 Pages: 766-70
  • Peer Reviewed
• [Presentation] TNF-aは骨芽細胞においてPOEMの発現を抑制し,骨芽細胞分化を制御する (2011)
  • Author(s): 塚崎雅之,山田 篤,高見正道,鈴木 大,相澤 怜,宮園あがさ,吉村健太郎,山本松男,上條竜太郎
  • Organizer: 第29回日本骨代謝学会学術集会
  • Place of Presentation: 大阪
• [Presentation] TGF-ssによるエナメル芽細胞分化におけるCaspase-14の役割 (2011)
  • Author(s): 宮園あがさ,須澤徹夫,山本 剛,小野美樹,相澤 怜,臼井通彦,立川哲彦,槇宏太郎,山本松男,上條竜太郎
  • Organizer: 第53回歯科基礎医学会学術大会
  • Place of Presentation: 岐阜
• [Presentation] TNF-aはPOEMの発現を抑制し,骨芽細胞分化を制御する (2011)
  • Author(s): 塚崎雅之,山田 篤,高見正道,鈴木 大,相澤 怜,宮園あがさ,吉村健太郎,山本松男,上條竜太郎
  • Organizer: 第53回歯科基礎医学会学術大会
  • Place of Presentation: 岐阜
• [Presentation] TGF-βによるエナメル芽細胞分化におけるCaspase-14の役割 (2011)
  • Author(s): 宮園あがさ, 須澤徹夫, 山本剛, 小野美樹, 相澤怜, 臼井通彦, 立川哲彦, 槇宏太郎, 山本松男, 上條竜太郎
  • Organizer: 第５３回歯科基礎医学会
  • Place of Presentation: 岐阜