| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2013: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2012: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2011: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Outline of Final Research Achievements |
This study aimed to elucidate, in vivo, the metabolism of several substrates in the oral biofilm and the actual influences of medicines such as fluoride and sugar substitutes such as xylitol to them through the metabolomic analysis approach using CE-TOFMS.
Consequently, it showed that, as well as sugar, the amino acids, especially glutamine and glutamate, are actually used as substrates in the oral biofilm. Moreover, it suggested that each amino acid is fermented by its respectively characteristic metabolism. The study also showed that fluoride can influence the sugar metabolism, including the inhibition of enolase and acid production, in the real oral biofilm as well, and suggested that fluoride affects some other metabolic reactions. In contrast, xylitol was suggested to have almost no effect on acid production and the metabolome profile in the oral biofilm, although xylitol 5-phosphate was produced.
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