The elucidation of the pathogenic activity of oral biofilm by Metabolomics approach
| Project/Area Number |
23792498
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Social dentistry
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| Research Institution | Tohoku University |
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Principal Investigator |
WASHIO Jumpei 東北大学, 歯学研究科(研究院), 助教 (20400260)
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| Project Period (FY) |
2011-04-28 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2013: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2012: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2011: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | メタボローム / バイオフィルム / 代謝 / 細菌 / 糖質 / 代用糖 / アミノ酸 / う蝕 / 亜硝酸 |
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| Outline of Final Research Achievements |
This study aimed to elucidate, in vivo, the metabolism of several substrates in the oral biofilm and the actual influences of medicines such as fluoride and sugar substitutes such as xylitol to them through the metabolomic analysis approach using CE-TOFMS.
Consequently, it showed that, as well as sugar, the amino acids, especially glutamine and glutamate, are actually used as substrates in the oral biofilm. Moreover, it suggested that each amino acid is fermented by its respectively characteristic metabolism. The study also showed that fluoride can influence the sugar metabolism, including the inhibition of enolase and acid production, in the real oral biofilm as well, and suggested that fluoride affects some other metabolic reactions. In contrast, xylitol was suggested to have almost no effect on acid production and the metabolome profile in the oral biofilm, although xylitol 5-phosphate was produced.
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Report
(5 results)
Research Products
(47 results)
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[Book] Interface Oral Health Science 20112012
Author(s)
Hitomi Domon-Tawaraya, Kazuko Nakajo, Jumpei Washio, Satoshi Fukumoto, Nobuhiro Takahashi
Publisher
Divalent Cations Enhance Short-Time Fluoride Exposure-Induced Inhibition on Acid Production by Streptococcus
Related Report
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[Book] Interface Oral Health Science 20112012
Author(s)
Junko Kawashima, Kazuko Nakajo, Jumpei Washio, Hidetoshi Shimauchi, Nobuhiro Takahashi
Publisher
Bicarbonate Decreases Inhibitory Effects of Fluoride on Acid Production by Actinomyces naeslundii
Related Report
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[Book] Interface Oral Health Science 20112012
Author(s)
Gen Mayanagi, Koei Igarashi, Jumpei Washio, Kazuko Nakajo, Hitomi Domon-Tawaraya, Nobuhiro Takahashi
Publisher
A New Method to Evaluate pH at the Interface Between Parasites and Biomaterials
Related Report
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[Book] Interface Oral Health Science 20112012
Author(s)
Yoko Sakuma, Jumpei Washio, Yasuhisa Takeuchi, Keiichi Sasaki, Nobuhiro Takahashi
Publisher
A Highly Sensitive alamarBlue Method for Evaluating Bacteial Adhesion to Biomaterials
Related Report
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